Team:Minnesota/Notebook

From 2011.igem.org

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__NOTOC__
{{Team:Minnesota/Top}}
{{Team:Minnesota/Top}}
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<br>=iGEM 2011 Laboratory Notebook=
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 +
==Project: Regulatory==
 +
===Date: Tuesday, 29 March 2011===
 +
{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
 +
| Tue, 29 Mar 2011 12:00:00 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-03292011120000-1|Transformation of comp. cells with registry parts]]
 +
|}
 +
===Date: Thursday, 07 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 07 Apr 2011 15:14:00 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-04072011151400-3|EnvZ mutant comp. cells made]]
 +
|}
 +
===Date: Monday, 18 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
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|-
 +
| Mon, 18 Apr 2011 12:00:00 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-04182011120000-4|Registry parts and results of X-gal assay of I15008]]
 +
|}
 +
===Date: Thursday, 21 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 21 Apr 2011 12:00:00 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-04212011120000-5|Transformation of pCph8 from Voigt lab]]
 +
|}
 +
===Date: Thursday, 26 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 26 May 2011 12:00:00 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-05262011120000-7|PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep]]
 +
|}
 +
===Date: Monday, 18 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 18 Jul 2011 14:59:48 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-07182011145948-2|Transformation of comp. cell with registry parts]]
 +
|-
 +
| Mon, 18 Jul 2011 15:28:28 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-07182011152828-8|Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet]]
 +
|}
 +
===Date: Wednesday, 20 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 20 Jul 2011 16:14:10 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-07202011161410-9|Miniprep of pUCbb-pBad and pUCbb-pTet]]
 +
|}
 +
===Date: Friday, 22 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 22 Jul 2011 19:58:49 GMT
 +
| Sung Bong Lee
 +
| [[Team:Minnesota/leex3772-07222011195849-10|run gel with pcr product of ho1 and cph 8 ]]
 +
|}
 +
===Date: Monday, 25 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 25 Jul 2011 14:23:53 GMT
 +
| Sung Bong Lee
 +
| [[Team:Minnesota/leex3772-07252011142353-11|Gel extraction of PCR product of ho1 and Cph8]]
 +
|}
 +
===Date: Thursday, 28 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 28 Jul 2011 20:25:02 GMT
 +
| Sung Bong Lee
 +
| [[Team:Minnesota/leex3772-07282011202502-12|Run gel with NEW PCR product of ho1 and cph8]]
 +
|}
 +
===Date: Wednesday, 03 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 03 Aug 2011 16:41:47 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08032011164147-13|Gel extraction of Cph8 and ho1]]
 +
|-
 +
| Wed, 03 Aug 2011 16:44:51 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08032011164451-14|Digest of ho1 and Cph8]]
 +
|}
 +
===Date: Wednesday, 10 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 10 Aug 2011 14:59:42 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08102011145942-15|Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8]]
 +
|}
 +
===Date: Saturday, 13 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 13 Aug 2011 12:35:20 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08132011123520-16|Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1]]
 +
|}
 +
===Date: Tuesday, 16 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 16 Aug 2011 13:55:44 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08162011135544-17|Digest of pcyA and ho1]]
 +
|}
 +
===Date: Tuesday, 23 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 23 Aug 2011 15:50:51 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08232011155051-72|Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1]]
 +
|}
 +
===Date: Tuesday, 30 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 30 Aug 2011 13:22:13 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08302011132213-114|Gel extraction and ligation of pcyA and ho1]]
 +
|-
 +
| Tue, 30 Aug 2011 13:29:13 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08302011132913-115|PCR of ompR and silicatein]]
 +
|}
 +
===Date: Thursday, 01 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 01 Sep 2011 14:14:48 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09012011141448-116|Gel extraction of ompR and silicatein]]
 +
|-
 +
| Thu, 01 Sep 2011 14:18:25 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09012011141825-117|Transformation of pcyA/ho1 ligation product]]
 +
|}
 +
===Date: Friday, 09 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 09 Sep 2011 11:45:17 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09092011114517-118|Miniprep of pUC-pcyA-ho1]]
 +
|-
 +
| Fri, 09 Sep 2011 12:13:01 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09092011121301-119|Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8]]
 +
|}
 +
===Date: Saturday, 10 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 10 Sep 2011 13:02:58 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09102011130258-120|Transformation of pUC-pcyA-ho1-Cph8]]
 +
|-
 +
| Sat, 10 Sep 2011 13:05:03 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09102011130503-121|Miniprep of pUCBB-lacZ and pBBR]]
 +
|-
 +
| Sat, 10 Sep 2011 13:06:54 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09102011130654-122|Digest of pUCBB-lacZ, pBBR, and ompR]]
 +
|}
 +
==Project: Surface Display==
 +
===Date: Thursday, 07 April 2011===
 +
{| class="wikitable"
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|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 07 Apr 2011 11:20:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04072011112000-18|Primer Design for OmpA]]
 +
|}
 +
===Date: Sunday, 10 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 10 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04102011000000-19|OmpA BL21 Genomic Liquid Cultures]]
 +
|}
 +
===Date: Monday, 11 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 11 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04112011000000-20|OmpA BL21 Genomic Extraction]]
 +
|}
 +
===Date: Thursday, 14 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-21|PCR Amplification of OmpA]]
 +
|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-22|pucBB Liquid Cultures]]
 +
|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-23|Gel Electrophoresis of OmpA PCR Product]]
 +
|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-24|Gel Purification of OmpA PCR Products]]
 +
|}
 +
===Date: Friday, 15 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 15 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04152011000000-25|Miniprep of pucBB+GFP]]
 +
|-
 +
| Fri, 15 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04152011000000-26|Restriction Digest of pucBB+GFP and Gel extraction of OmpA]]
 +
|}
 +
===Date: Saturday, 16 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 16 Apr 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04162011000100-27|Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP]]
 +
|-
 +
| Sat, 16 Apr 2011 00:02:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04162011000200-28|Restriction Digest of pucBB and ompA inserts]]
 +
|-
 +
| Sat, 16 Apr 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04162011000300-29|Gel Electrophoresis of OmpA ]]
 +
|}
 +
===Date: Tuesday, 19 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 19 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04192011000000-30|PCR Amplification of Genomic BL21]]
 +
|-
 +
| Tue, 19 Apr 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04192011000100-31|Gel Extraction of OmpA]]
 +
|}
 +
===Date: Wednesday, 20 April 2011===
 +
{| class="wikitable"
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|-
 +
! Date
 +
! Author
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! Subject
 +
|-
 +
| Wed, 20 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04202011000000-32|pBAD plasmid liquid cultures]]
 +
|}
 +
===Date: Thursday, 21 April 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
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|-
 +
| Thu, 21 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04212011000000-33|Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction]]
 +
|-
 +
| Thu, 21 Apr 2011 12:00:00 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-04212011120000-6|Gel extraction of Silicatein alpha DNA]]
 +
|}
 +
===Date: Friday, 22 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 22 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04222011000000-40|Miniprep, Digest, and Extract]]
 +
|}
 +
===Date: Saturday, 23 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 23 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04232011000000-35|Ligation of pBAD and OmpA]]
 +
|}
 +
===Date: Tuesday, 26 April 2011===
 +
{| class="wikitable"
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|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 26 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04262011000000-36|Restriction digest of OmpA, PCR of OmpA (back-up)]]
 +
|-
 +
| Tue, 26 Apr 2011 15:52:22 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-04262011155222-153|PCR set up]]
 +
|-
 +
| Tue, 26 Apr 2011 16:02:53 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-04262011160253-154|PCR  trial #2]]
 +
|}
 +
===Date: Thursday, 28 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 28 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04282011000000-37|PCR of OmpA and Gel]]
 +
|}
 +
===Date: Saturday, 30 April 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 30 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04302011000000-38|Speed Vacuum]]
 +
|}
 +
===Date: Tuesday, 03 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 03 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05032011000000-39|Restriction Digest of pBAD and OmpA insert]]
 +
|}
 +
===Date: Wednesday, 04 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 04 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05042011000000-41|PCR purification of OmpA and Gel Extraction]]
 +
|}
 +
===Date: Thursday, 05 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 05 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05052011000000-42|Ligation of OmpA and pBAD]]
 +
|}
 +
===Date: Friday, 06 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 06 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05062011000000-43|Transformation]]
 +
|}
 +
===Date: Monday, 23 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 23 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05232011000000-44|Transformation and Liquid cultures of OmpA transformants]]
 +
|}
 +
===Date: Tuesday, 24 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 24 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05242011000000-45|Miniprep and PCR, Liquid Cultures ]]
 +
|}
 +
===Date: Wednesday, 25 May 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 25 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05252011000000-46|Gel of OmpA Plasmid]]
 +
|}
 +
===Date: Wednesday, 01 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 01 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06012011000000-47|Liquid cultures of pBAD plasmid]]
 +
|}
 +
===Date: Thursday, 02 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 02 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06022011000000-48|PCR Amplification of OmpA and Miniprep of pBADBB]]
 +
|}
 +
===Date: Friday, 03 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 03 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06032011000000-49|Gel Electrophoresis of OmpA and pBAD PCR Products and Digests]]
 +
|-
 +
| Fri, 03 Jun 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06032011000100-50|Extractions of OmpA]]
 +
|}
 +
===Date: Sunday, 05 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 05 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06052011000000-51|Speed Vac and Digests]]
 +
|}
 +
===Date: Monday, 06 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 06 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06062011000000-52|Gel and Extraction]]
 +
|}
 +
===Date: Tuesday, 07 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 07 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06072011000000-53|Digestion of OmpA]]
 +
|}
 +
===Date: Wednesday, 08 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 08 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06082011000000-54|PCR Purification/Gel Extraction]]
 +
|}
 +
===Date: Thursday, 09 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 09 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06092011000000-55|Ligation of OmpA and pBAD]]
 +
|}
 +
===Date: Friday, 10 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 10 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06102011000000-56|Transformation of OmpA plasmid ]]
 +
|}
 +
===Date: Sunday, 12 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 12 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06122011000000-57|Electroporation transformation]]
 +
|}
 +
===Date: Monday, 13 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 13 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06132011000000-58|Ligation of OmpA+pBAD]]
 +
|}
 +
===Date: Tuesday, 14 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 14 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06142011000000-59|Ligation]]
 +
|}
 +
===Date: Wednesday, 15 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 15 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06152011000000-60|Electroporation Transformation]]
 +
|}
 +
===Date: Saturday, 18 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 18 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06182011000000-61|PCR of OmpA]]
 +
|}
 +
===Date: Monday, 20 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 20 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06202011000000-62|TOPO Cloning and Gel Extraction]]
 +
|}
 +
===Date: Tuesday, 21 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 21 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06212011000000-63|Liquid Cultures of TOPO Cloning]]
 +
|}
 +
===Date: Wednesday, 22 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 22 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06222011000000-64|GoGreen PCR and Miniprep of TOPO Liquid Cultures]]
 +
|}
 +
===Date: Thursday, 23 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 23 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06232011000000-65|Restriction Digests]]
 +
|}
 +
===Date: Friday, 24 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 24 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06242011000000-66|Gel Extraction and Ligation]]
 +
|}
 +
===Date: Saturday, 25 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 25 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06252011000000-67|Electroporation]]
 +
|-
 +
| Sat, 25 Jun 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06252011000100-68|Chemical Transformation]]
 +
|}
 +
===Date: Sunday, 26 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 26 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06262011000000-69|Liquid cultures of OmpA plasmids]]
 +
|}
 +
===Date: Monday, 27 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 27 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06272011000000-70|Miniprep of OmpA and pBAD plasmid]]
 +
|}
 +
===Date: Tuesday, 28 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 28 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06282011000000-71|Gogreen PCR and Digestion Check]]
 +
|-
 +
| Tue, 28 Jun 2011 16:10:19 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-06282011161019-155|PCR of INP]]
 +
|}
 +
===Date: Wednesday, 29 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 29 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06292011000000-73|Silicatein: Digest of pucBB]]
 +
|-
 +
| Wed, 29 Jun 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06292011000100-74|Ligation of pucBB plasmid and Silicatein]]
 +
|}
 +
===Date: Thursday, 30 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 30 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06302011000000-75|Transformation]]
 +
|}
 +
===Date: Friday, 01 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 01 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07012011000000-76|Liquid cultures of pucBB+Silicatein Transformants]]
 +
|}
 +
===Date: Saturday, 02 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 02 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07022011000000-77|Miniprep of Silicatein+pucBB transformants]]
 +
|}
 +
===Date: Monday, 04 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 04 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07042011000000-78|Liquid Gogreen PCR ]]
 +
|}
 +
===Date: Wednesday, 06 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 06 Jul 2011 16:15:53 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07062011161553-156|INP PCR clean up]]
 +
|}
 +
===Date: Thursday, 07 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 07 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07072011000000-79|Gogreen of Silicatein pucBB plasmid]]
 +
|-
 +
| Thu, 07 Jul 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07072011000100-80|Restriction Digest of pucBB and PCR of Silicatein]]
 +
|}
 +
===Date: Friday, 08 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 08 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07082011000000-81|Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation]]
 +
|}
 +
===Date: Monday, 11 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 11 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07112011000000-82|Gel Extraction of Silicatein and Liquid Cultures of pucBB]]
 +
|}
 +
===Date: Tuesday, 12 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 12 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07122011000000-83|Transformation of pucBB+Silicatein]]
 +
|-
 +
| Tue, 12 Jul 2011 16:17:17 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07122011161717-157|Digestion of INP]]
 +
|}
 +
===Date: Wednesday, 13 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 13 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07132011000000-84|Gogreen PCR and Miniprep]]
 +
|-
 +
| Wed, 13 Jul 2011 16:20:04 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07132011162004-158|INP cloning]]
 +
|}
 +
===Date: Friday, 15 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 15 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07152011000000-85|pucBB Digest and pucBB Liquid Cultures]]
 +
|}
 +
===Date: Saturday, 16 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 16 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07162011000000-86|Miniprep of pucBB and gel extraction of pucBB]]
 +
|}
 +
===Date: Wednesday, 20 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 20 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07202011000000-87|PCR of Silicatein, Gel Extraction, and TOPO Cloning]]
 +
|-
 +
| Wed, 20 Jul 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07202011000100-88|TOPO Transformants and Liquid Cultures]]
 +
|-
 +
| Wed, 20 Jul 2011 17:59:22 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07202011175922-159|Transformation trial #2]]
 +
|}
 +
===Date: Friday, 22 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 22 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07222011000000-89|TOPO Miniprep and Gogreen PCR and Gel]]
 +
|}
 +
===Date: Monday, 25 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 25 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07252011000000-90|Gogreen PCR X3 and Gel X3]]
 +
|}
 +
===Date: Tuesday, 26 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 26 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07262011000000-91|PCR of Silicatein and TOPO]]
 +
|-
 +
| Tue, 26 Jul 2011 18:02:01 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07262011180201-160|INP PCR with Taq mix]]
 +
|}
 +
===Date: Wednesday, 27 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 27 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07272011000000-92|Liquid Cultures and Colony Gogreen PCR]]
 +
|-
 +
| Wed, 27 Jul 2011 18:06:41 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07272011180641-161|INP PCR w/Taq & digestion]]
 +
|}
 +
===Date: Thursday, 28 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 28 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07282011000000-93|Miniprep and Sequencing of the TOPO Silicatein]]
 +
|}
 +
===Date: Tuesday, 02 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 02 Aug 2011 18:12:35 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08022011181235-162|Ligation & transformation ]]
 +
|}
 +
===Date: Wednesday, 03 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 03 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08032011000000-94|Digestion of Silicatein TOPO 7]]
 +
|}
 +
===Date: Thursday, 04 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 04 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08042011000000-95|Gel Extraction, Ligation, and Transformation]]
 +
|}
 +
===Date: Monday, 08 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 08 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08082011000000-96|Liquid Cultures, Master Plate, Gogreen, and Gel]]
 +
|}
 +
===Date: Tuesday, 09 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 09 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08092011000000-97|Miniprep, Gogreen Liquid PCR, and Gel]]
 +
|}
 +
===Date: Wednesday, 10 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 10 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08102011000000-98|Liquid Cultures, Liquid Gogreen PCR]]
 +
|}
 +
===Date: Thursday, 11 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 11 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08112011000000-99|More Liquid Cultures, Liquid Gogreen PCR]]
 +
|}
 +
===Date: Friday, 12 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 12 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08122011000000-100|Transformation of TOPO Silicatein Back-up]]
 +
|}
 +
===Date: Saturday, 13 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 13 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08132011000000-101|Liquid Cultures of Back-Up Silicatein TOPO]]
 +
|}
 +
===Date: Sunday, 14 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 14 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08142011000000-102|Miniprep of Silicatein TOPO]]
 +
|}
 +
===Date: Monday, 15 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 15 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08152011000000-103|Digest of pucBB and Silicatein TOPO]]
 +
|}
 +
===Date: Tuesday, 16 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 16 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08162011000000-104|Miniprep of pucBB and Gel Extraction]]
 +
|-
 +
| Tue, 16 Aug 2011 18:14:41 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08162011181441-163|Digestion]]
 +
|}
 +
===Date: Wednesday, 17 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 17 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08172011000000-105|Liquid Cultures of Transformants and Gogreen]]
 +
|}
 +
===Date: Thursday, 18 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 18 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08182011000000-106|Gogreen PCR of Liquid Cultures]]
 +
|}
 +
===Date: Friday, 19 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 19 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08192011000000-107|Gogreen PCR of Liquid Cultures]]
 +
|}
 +
===Date: Monday, 22 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 22 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08222011000000-108|Ligation and Transformation]]
 +
|-
 +
| Mon, 22 Aug 2011 10:15:36 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08222011101536-34|Minnesota/leex4462-08222011101536-34]]
 +
|}
 +
===Date: Tuesday, 23 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 23 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08232011000000-109|Ligation of pBAD+Silicatein and Digest of TOPO Silicatein]]
 +
|-
 +
| Tue, 23 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08232011000000-110|Liquid Cultures, Gogreen PCR of Silicatein+pBAD]]
 +
|}
 +
===Date: Wednesday, 24 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 24 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08242011000000-111|pBAD+Silicatein transformation and gel extraction of TOPO Silicatein]]
 +
|-
 +
| Wed, 24 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08242011000000-123|Ligation, Digest, Transformation of pBAD]]
 +
|}
 +
===Date: Thursday, 25 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 25 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08252011000000-112|Liquid Cultures, Gogreen PCR of Silicatein+pBAD]]
 +
|-
 +
| Thu, 25 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08252011000000-124|Minnesota/leex4462-08252011000000-124]]
 +
|-
 +
| Thu, 25 Aug 2011 18:25:40 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08252011182540-165| Cloning #3]]
 +
|}
 +
===Date: Friday, 26 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 26 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08262011000000-113|Miniprep and Gogreen Check]]
 +
|-
 +
| Fri, 26 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08262011000000-125|Minnesota/leex4462-08262011000000-125]]
 +
|}
 +
===Date: Saturday, 27 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 27 Aug 2011 18:17:00 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08272011181700-164|Cloning]]
 +
|}
 +
===Date: Monday, 29 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 29 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08292011000000-126|Ligation, Digest, Transformation of pBAD]]
 +
|-
 +
| Mon, 29 Aug 2011 18:30:52 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08292011183052-166|Verifying clones]]
 +
|}
 +
===Date: Tuesday, 30 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 30 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08302011000000-127|Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation]]
 +
|-
 +
| Tue, 30 Aug 2011 18:38:12 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08302011183812-167|SiL & GFP PCT]]
 +
|}
 +
===Date: Wednesday, 31 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 31 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08312011000000-128|Miniprep of pBAD]]
 +
|}
 +
===Date: Friday, 02 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 02 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09022011000000-129|PCR Overlap Extension (OmpA+Silicatein Fusion)]]
 +
|-
 +
| Fri, 02 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09022011000100-130|Gel of PCR Overlap]]
 +
|-
 +
| Fri, 02 Sep 2011 00:02:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09022011000200-131|PCR Overlap Test]]
 +
|}
 +
===Date: Tuesday, 06 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 06 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09062011000000-132|Gel of PCR Overlap ]]
 +
|-
 +
| Tue, 06 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09062011000100-133|PCR Overlap Extension]]
 +
|-
 +
| Tue, 06 Sep 2011 18:47:17 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-09062011184717-168|Further cloning]]
 +
|}
 +
===Date: Wednesday, 07 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 07 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09072011000000-134|Gel of PCR Products/Gel Extraction]]
 +
|}
 +
===Date: Thursday, 08 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 08 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000000-135|PCR Overlap Extension]]
 +
|-
 +
| Thu, 08 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000100-136|Gel Extraction of the PCR Overlap Extension]]
 +
|-
 +
| Thu, 08 Sep 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000300-137|Transformation of pBAD+ OmpA]]
 +
|-
 +
| Thu, 08 Sep 2011 00:04:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000400-138|PCR of Silicatein]]
 +
|-
 +
| Thu, 08 Sep 2011 18:51:05 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-09082011185105-169|More cloning]]
 +
|}
 +
===Date: Friday, 09 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 09 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000000-139|Part II of PCR Overlap]]
 +
|-
 +
| Fri, 09 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000100-140|Liquid Cultures of pBAD and pBAD+OmpA]]
 +
|-
 +
| Fri, 09 Sep 2011 00:02:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000200-141|PCR of Silicatein ]]
 +
|-
 +
| Fri, 09 Sep 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000300-142|Gel of Part2+3 and Test of Part 3]]
 +
|}
 +
===Date: Saturday, 10 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 10 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000000-143|Miniprep of OmpA+pBAD and pBAD]]
 +
|-
 +
| Sat, 10 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000100-144|Gel Extraction of Part 3]]
 +
|-
 +
| Sat, 10 Sep 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000300-145|Gel of PCR Silicatein]]
 +
|-
 +
| Sat, 10 Sep 2011 00:04:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000400-146|PCR Overlap Check]]
 +
|}
 +
===Date: Monday, 12 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 12 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09122011000000-147|Gel of PCR Products]]
 +
|-
 +
| Mon, 12 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09122011000100-148|PCR of Part 3 and Silicatein]]
 +
|-
 +
| Mon, 12 Sep 2011 18:54:16 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-09122011185416-170|Verifying clones]]
 +
|}
 +
===Date: Tuesday, 13 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 13 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09132011000000-149|PCR of Part 3]]
 +
|}
 +
===Date: Wednesday, 14 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 14 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09142011000000-150|Gel of PCR]]
 +
|}
 +
===Date: Friday, 16 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 16 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09162011000000-151|PCR of Silicatein, part 1, part S, part 2, and part 3]]
 +
|-
 +
| Fri, 16 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09162011000100-152|Gel of PCR Products+Extraction]]
 +
|}
{{Team:Minnesota/Bottom}}
{{Team:Minnesota/Bottom}}

Revision as of 22:41, 27 September 2011

Mnlogo.jpg
Home Team Project Software Protocols Notebook Attributions Safety

=iGEM 2011 Laboratory Notebook=

Project: Regulatory

Date: Tuesday, 29 March 2011

Date Author Subject
Tue, 29 Mar 2011 12:00:00 GMT Nathan Davis Transformation of comp. cells with registry parts

Date: Thursday, 07 April 2011

Date Author Subject
Thu, 07 Apr 2011 15:14:00 GMT Nathan Davis EnvZ mutant comp. cells made

Date: Monday, 18 April 2011

Date Author Subject
Mon, 18 Apr 2011 12:00:00 GMT Nathan Davis Registry parts and results of X-gal assay of I15008

Date: Thursday, 21 April 2011

Date Author Subject
Thu, 21 Apr 2011 12:00:00 GMT Nathan Davis Transformation of pCph8 from Voigt lab

Date: Thursday, 26 May 2011

Date Author Subject
Thu, 26 May 2011 12:00:00 GMT Nathan Davis PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep

Date: Monday, 18 July 2011

Date Author Subject
Mon, 18 Jul 2011 14:59:48 GMT Nathan Davis Transformation of comp. cell with registry parts
Mon, 18 Jul 2011 15:28:28 GMT Nathan Davis Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet

Date: Wednesday, 20 July 2011

Date Author Subject
Wed, 20 Jul 2011 16:14:10 GMT Nathan Davis Miniprep of pUCbb-pBad and pUCbb-pTet

Date: Friday, 22 July 2011

Date Author Subject
Fri, 22 Jul 2011 19:58:49 GMT Sung Bong Lee run gel with pcr product of ho1 and cph 8

Date: Monday, 25 July 2011

Date Author Subject
Mon, 25 Jul 2011 14:23:53 GMT Sung Bong Lee Gel extraction of PCR product of ho1 and Cph8

Date: Thursday, 28 July 2011

Date Author Subject
Thu, 28 Jul 2011 20:25:02 GMT Sung Bong Lee Run gel with NEW PCR product of ho1 and cph8

Date: Wednesday, 03 August 2011

Date Author Subject
Wed, 03 Aug 2011 16:41:47 GMT Nathan Davis Gel extraction of Cph8 and ho1
Wed, 03 Aug 2011 16:44:51 GMT Nathan Davis Digest of ho1 and Cph8

Date: Wednesday, 10 August 2011

Date Author Subject
Wed, 10 Aug 2011 14:59:42 GMT Nathan Davis Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8

Date: Saturday, 13 August 2011

Date Author Subject
Sat, 13 Aug 2011 12:35:20 GMT Nathan Davis Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1

Date: Tuesday, 16 August 2011

Date Author Subject
Tue, 16 Aug 2011 13:55:44 GMT Nathan Davis Digest of pcyA and ho1

Date: Tuesday, 23 August 2011

Date Author Subject
Tue, 23 Aug 2011 15:50:51 GMT Nathan Davis Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1

Date: Tuesday, 30 August 2011

Date Author Subject
Tue, 30 Aug 2011 13:22:13 GMT Nathan Davis Gel extraction and ligation of pcyA and ho1
Tue, 30 Aug 2011 13:29:13 GMT Nathan Davis PCR of ompR and silicatein

Date: Thursday, 01 September 2011

Date Author Subject
Thu, 01 Sep 2011 14:14:48 GMT Nathan Davis Gel extraction of ompR and silicatein
Thu, 01 Sep 2011 14:18:25 GMT Nathan Davis Transformation of pcyA/ho1 ligation product

Date: Friday, 09 September 2011

Date Author Subject
Fri, 09 Sep 2011 11:45:17 GMT Nathan Davis Miniprep of pUC-pcyA-ho1
Fri, 09 Sep 2011 12:13:01 GMT Nathan Davis Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8

Date: Saturday, 10 September 2011

Date Author Subject
Sat, 10 Sep 2011 13:02:58 GMT Nathan Davis Transformation of pUC-pcyA-ho1-Cph8
Sat, 10 Sep 2011 13:05:03 GMT Nathan Davis Miniprep of pUCBB-lacZ and pBBR
Sat, 10 Sep 2011 13:06:54 GMT Nathan Davis Digest of pUCBB-lacZ, pBBR, and ompR

Project: Surface Display

Date: Thursday, 07 April 2011

Date Author Subject
Thu, 07 Apr 2011 11:20:00 GMT Sukdong Lee Primer Design for OmpA

Date: Sunday, 10 April 2011

Date Author Subject
Sun, 10 Apr 2011 00:00:00 GMT Sukdong Lee OmpA BL21 Genomic Liquid Cultures

Date: Monday, 11 April 2011

Date Author Subject
Mon, 11 Apr 2011 00:00:00 GMT Sukdong Lee OmpA BL21 Genomic Extraction

Date: Thursday, 14 April 2011

Date Author Subject
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee PCR Amplification of OmpA
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee pucBB Liquid Cultures
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee Gel Electrophoresis of OmpA PCR Product
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee Gel Purification of OmpA PCR Products

Date: Friday, 15 April 2011

Date Author Subject
Fri, 15 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB+GFP
Fri, 15 Apr 2011 00:00:00 GMT Sukdong Lee Restriction Digest of pucBB+GFP and Gel extraction of OmpA

Date: Saturday, 16 April 2011

Date Author Subject
Sat, 16 Apr 2011 00:01:00 GMT Sukdong Lee Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP
Sat, 16 Apr 2011 00:02:00 GMT Sukdong Lee Restriction Digest of pucBB and ompA inserts
Sat, 16 Apr 2011 00:03:00 GMT Sukdong Lee Gel Electrophoresis of OmpA

Date: Tuesday, 19 April 2011

Date Author Subject
Tue, 19 Apr 2011 00:00:00 GMT Sukdong Lee PCR Amplification of Genomic BL21
Tue, 19 Apr 2011 00:01:00 GMT Sukdong Lee Gel Extraction of OmpA

Date: Wednesday, 20 April 2011

Date Author Subject
Wed, 20 Apr 2011 00:00:00 GMT Sukdong Lee pBAD plasmid liquid cultures

Date: Thursday, 21 April 2011

Date Author Subject
Thu, 21 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction
Thu, 21 Apr 2011 12:00:00 GMT Nathan Davis Gel extraction of Silicatein alpha DNA

Date: Friday, 22 April 2011

Date Author Subject
Fri, 22 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep, Digest, and Extract

Date: Saturday, 23 April 2011

Date Author Subject
Sat, 23 Apr 2011 00:00:00 GMT Sukdong Lee Ligation of pBAD and OmpA

Date: Tuesday, 26 April 2011

Date Author Subject
Tue, 26 Apr 2011 00:00:00 GMT Sukdong Lee Restriction digest of OmpA, PCR of OmpA (back-up)
Tue, 26 Apr 2011 15:52:22 GMT Viki Ding PCR set up
Tue, 26 Apr 2011 16:02:53 GMT Viki Ding PCR trial #2

Date: Thursday, 28 April 2011

Date Author Subject
Thu, 28 Apr 2011 00:00:00 GMT Sukdong Lee PCR of OmpA and Gel

Date: Saturday, 30 April 2011

Date Author Subject
Sat, 30 Apr 2011 00:00:00 GMT Sukdong Lee Speed Vacuum

Date: Tuesday, 03 May 2011

Date Author Subject
Tue, 03 May 2011 00:00:00 GMT Sukdong Lee Restriction Digest of pBAD and OmpA insert

Date: Wednesday, 04 May 2011

Date Author Subject
Wed, 04 May 2011 00:00:00 GMT Sukdong Lee PCR purification of OmpA and Gel Extraction

Date: Thursday, 05 May 2011

Date Author Subject
Thu, 05 May 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA and pBAD

Date: Friday, 06 May 2011

Date Author Subject
Fri, 06 May 2011 00:00:00 GMT Sukdong Lee Transformation

Date: Monday, 23 May 2011

Date Author Subject
Mon, 23 May 2011 00:00:00 GMT Sukdong Lee Transformation and Liquid cultures of OmpA transformants

Date: Tuesday, 24 May 2011

Date Author Subject
Tue, 24 May 2011 00:00:00 GMT Sukdong Lee Miniprep and PCR, Liquid Cultures

Date: Wednesday, 25 May 2011

Date Author Subject
Wed, 25 May 2011 00:00:00 GMT Sukdong Lee Gel of OmpA Plasmid

Date: Wednesday, 01 June 2011

Date Author Subject
Wed, 01 Jun 2011 00:00:00 GMT Sukdong Lee Liquid cultures of pBAD plasmid

Date: Thursday, 02 June 2011

Date Author Subject
Thu, 02 Jun 2011 00:00:00 GMT Sukdong Lee PCR Amplification of OmpA and Miniprep of pBADBB

Date: Friday, 03 June 2011

Date Author Subject
Fri, 03 Jun 2011 00:00:00 GMT Sukdong Lee Gel Electrophoresis of OmpA and pBAD PCR Products and Digests
Fri, 03 Jun 2011 00:01:00 GMT Sukdong Lee Extractions of OmpA

Date: Sunday, 05 June 2011

Date Author Subject
Sun, 05 Jun 2011 00:00:00 GMT Sukdong Lee Speed Vac and Digests

Date: Monday, 06 June 2011

Date Author Subject
Mon, 06 Jun 2011 00:00:00 GMT Sukdong Lee Gel and Extraction

Date: Tuesday, 07 June 2011

Date Author Subject
Tue, 07 Jun 2011 00:00:00 GMT Sukdong Lee Digestion of OmpA

Date: Wednesday, 08 June 2011

Date Author Subject
Wed, 08 Jun 2011 00:00:00 GMT Sukdong Lee PCR Purification/Gel Extraction

Date: Thursday, 09 June 2011

Date Author Subject
Thu, 09 Jun 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA and pBAD

Date: Friday, 10 June 2011

Date Author Subject
Fri, 10 Jun 2011 00:00:00 GMT Sukdong Lee Transformation of OmpA plasmid

Date: Sunday, 12 June 2011

Date Author Subject
Sun, 12 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation transformation

Date: Monday, 13 June 2011

Date Author Subject
Mon, 13 Jun 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA+pBAD

Date: Tuesday, 14 June 2011

Date Author Subject
Tue, 14 Jun 2011 00:00:00 GMT Sukdong Lee Ligation

Date: Wednesday, 15 June 2011

Date Author Subject
Wed, 15 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation Transformation

Date: Saturday, 18 June 2011

Date Author Subject
Sat, 18 Jun 2011 00:00:00 GMT Sukdong Lee PCR of OmpA

Date: Monday, 20 June 2011

Date Author Subject
Mon, 20 Jun 2011 00:00:00 GMT Sukdong Lee TOPO Cloning and Gel Extraction

Date: Tuesday, 21 June 2011

Date Author Subject
Tue, 21 Jun 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of TOPO Cloning

Date: Wednesday, 22 June 2011

Date Author Subject
Wed, 22 Jun 2011 00:00:00 GMT Sukdong Lee GoGreen PCR and Miniprep of TOPO Liquid Cultures

Date: Thursday, 23 June 2011

Date Author Subject
Thu, 23 Jun 2011 00:00:00 GMT Sukdong Lee Restriction Digests

Date: Friday, 24 June 2011

Date Author Subject
Fri, 24 Jun 2011 00:00:00 GMT Sukdong Lee Gel Extraction and Ligation

Date: Saturday, 25 June 2011

Date Author Subject
Sat, 25 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation
Sat, 25 Jun 2011 00:01:00 GMT Sukdong Lee Chemical Transformation

Date: Sunday, 26 June 2011

Date Author Subject
Sun, 26 Jun 2011 00:00:00 GMT Sukdong Lee Liquid cultures of OmpA plasmids

Date: Monday, 27 June 2011

Date Author Subject
Mon, 27 Jun 2011 00:00:00 GMT Sukdong Lee Miniprep of OmpA and pBAD plasmid

Date: Tuesday, 28 June 2011

Date Author Subject
Tue, 28 Jun 2011 00:00:00 GMT Sukdong Lee Gogreen PCR and Digestion Check
Tue, 28 Jun 2011 16:10:19 GMT Viki Ding PCR of INP

Date: Wednesday, 29 June 2011

Date Author Subject
Wed, 29 Jun 2011 00:00:00 GMT Sukdong Lee Silicatein: Digest of pucBB
Wed, 29 Jun 2011 00:01:00 GMT Sukdong Lee Ligation of pucBB plasmid and Silicatein

Date: Thursday, 30 June 2011

Date Author Subject
Thu, 30 Jun 2011 00:00:00 GMT Sukdong Lee Transformation

Date: Friday, 01 July 2011

Date Author Subject
Fri, 01 Jul 2011 00:00:00 GMT Sukdong Lee Liquid cultures of pucBB+Silicatein Transformants

Date: Saturday, 02 July 2011

Date Author Subject
Sat, 02 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep of Silicatein+pucBB transformants

Date: Monday, 04 July 2011

Date Author Subject
Mon, 04 Jul 2011 00:00:00 GMT Sukdong Lee Liquid Gogreen PCR

Date: Wednesday, 06 July 2011

Date Author Subject
Wed, 06 Jul 2011 16:15:53 GMT Viki Ding INP PCR clean up

Date: Thursday, 07 July 2011

Date Author Subject
Thu, 07 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen of Silicatein pucBB plasmid
Thu, 07 Jul 2011 00:01:00 GMT Sukdong Lee Restriction Digest of pucBB and PCR of Silicatein

Date: Friday, 08 July 2011

Date Author Subject
Fri, 08 Jul 2011 00:00:00 GMT Sukdong Lee Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation

Date: Monday, 11 July 2011

Date Author Subject
Mon, 11 Jul 2011 00:00:00 GMT Sukdong Lee Gel Extraction of Silicatein and Liquid Cultures of pucBB

Date: Tuesday, 12 July 2011

Date Author Subject
Tue, 12 Jul 2011 00:00:00 GMT Sukdong Lee Transformation of pucBB+Silicatein
Tue, 12 Jul 2011 16:17:17 GMT Viki Ding Digestion of INP

Date: Wednesday, 13 July 2011

Date Author Subject
Wed, 13 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen PCR and Miniprep
Wed, 13 Jul 2011 16:20:04 GMT Viki Ding INP cloning

Date: Friday, 15 July 2011

Date Author Subject
Fri, 15 Jul 2011 00:00:00 GMT Sukdong Lee pucBB Digest and pucBB Liquid Cultures

Date: Saturday, 16 July 2011

Date Author Subject
Sat, 16 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB and gel extraction of pucBB

Date: Wednesday, 20 July 2011

Date Author Subject
Wed, 20 Jul 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein, Gel Extraction, and TOPO Cloning
Wed, 20 Jul 2011 00:01:00 GMT Sukdong Lee TOPO Transformants and Liquid Cultures
Wed, 20 Jul 2011 17:59:22 GMT Viki Ding Transformation trial #2

Date: Friday, 22 July 2011

Date Author Subject
Fri, 22 Jul 2011 00:00:00 GMT Sukdong Lee TOPO Miniprep and Gogreen PCR and Gel

Date: Monday, 25 July 2011

Date Author Subject
Mon, 25 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen PCR X3 and Gel X3

Date: Tuesday, 26 July 2011

Date Author Subject
Tue, 26 Jul 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein and TOPO
Tue, 26 Jul 2011 18:02:01 GMT Viki Ding INP PCR with Taq mix

Date: Wednesday, 27 July 2011

Date Author Subject
Wed, 27 Jul 2011 00:00:00 GMT Sukdong Lee Liquid Cultures and Colony Gogreen PCR
Wed, 27 Jul 2011 18:06:41 GMT Viki Ding INP PCR w/Taq & digestion

Date: Thursday, 28 July 2011

Date Author Subject
Thu, 28 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep and Sequencing of the TOPO Silicatein

Date: Tuesday, 02 August 2011

Date Author Subject
Tue, 02 Aug 2011 18:12:35 GMT Viki Ding Ligation & transformation

Date: Wednesday, 03 August 2011

Date Author Subject
Wed, 03 Aug 2011 00:00:00 GMT Sukdong Lee Digestion of Silicatein TOPO 7

Date: Thursday, 04 August 2011

Date Author Subject
Thu, 04 Aug 2011 00:00:00 GMT Sukdong Lee Gel Extraction, Ligation, and Transformation

Date: Monday, 08 August 2011

Date Author Subject
Mon, 08 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Master Plate, Gogreen, and Gel

Date: Tuesday, 09 August 2011

Date Author Subject
Tue, 09 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep, Gogreen Liquid PCR, and Gel

Date: Wednesday, 10 August 2011

Date Author Subject
Wed, 10 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Liquid Gogreen PCR

Date: Thursday, 11 August 2011

Date Author Subject
Thu, 11 Aug 2011 00:00:00 GMT Sukdong Lee More Liquid Cultures, Liquid Gogreen PCR

Date: Friday, 12 August 2011

Date Author Subject
Fri, 12 Aug 2011 00:00:00 GMT Sukdong Lee Transformation of TOPO Silicatein Back-up

Date: Saturday, 13 August 2011

Date Author Subject
Sat, 13 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of Back-Up Silicatein TOPO

Date: Sunday, 14 August 2011

Date Author Subject
Sun, 14 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of Silicatein TOPO

Date: Monday, 15 August 2011

Date Author Subject
Mon, 15 Aug 2011 00:00:00 GMT Sukdong Lee Digest of pucBB and Silicatein TOPO

Date: Tuesday, 16 August 2011

Date Author Subject
Tue, 16 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB and Gel Extraction
Tue, 16 Aug 2011 18:14:41 GMT Viki Ding Digestion

Date: Wednesday, 17 August 2011

Date Author Subject
Wed, 17 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of Transformants and Gogreen

Date: Thursday, 18 August 2011

Date Author Subject
Thu, 18 Aug 2011 00:00:00 GMT Sukdong Lee Gogreen PCR of Liquid Cultures

Date: Friday, 19 August 2011

Date Author Subject
Fri, 19 Aug 2011 00:00:00 GMT Sukdong Lee Gogreen PCR of Liquid Cultures

Date: Monday, 22 August 2011

Date Author Subject
Mon, 22 Aug 2011 00:00:00 GMT Sukdong Lee Ligation and Transformation
Mon, 22 Aug 2011 10:15:36 GMT Sukdong Lee Minnesota/leex4462-08222011101536-34

Date: Tuesday, 23 August 2011

Date Author Subject
Tue, 23 Aug 2011 00:00:00 GMT Sukdong Lee Ligation of pBAD+Silicatein and Digest of TOPO Silicatein
Tue, 23 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Gogreen PCR of Silicatein+pBAD

Date: Wednesday, 24 August 2011

Date Author Subject
Wed, 24 Aug 2011 00:00:00 GMT Sukdong Lee pBAD+Silicatein transformation and gel extraction of TOPO Silicatein
Wed, 24 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Digest, Transformation of pBAD

Date: Thursday, 25 August 2011

Date Author Subject
Thu, 25 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Gogreen PCR of Silicatein+pBAD
Thu, 25 Aug 2011 00:00:00 GMT Sukdong Lee Minnesota/leex4462-08252011000000-124
Thu, 25 Aug 2011 18:25:40 GMT Viki Ding Cloning #3

Date: Friday, 26 August 2011

Date Author Subject
Fri, 26 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep and Gogreen Check
Fri, 26 Aug 2011 00:00:00 GMT Sukdong Lee Minnesota/leex4462-08262011000000-125

Date: Saturday, 27 August 2011

Date Author Subject
Sat, 27 Aug 2011 18:17:00 GMT Viki Ding Cloning

Date: Monday, 29 August 2011

Date Author Subject
Mon, 29 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Digest, Transformation of pBAD
Mon, 29 Aug 2011 18:30:52 GMT Viki Ding Verifying clones

Date: Tuesday, 30 August 2011

Date Author Subject
Tue, 30 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation
Tue, 30 Aug 2011 18:38:12 GMT Viki Ding SiL & GFP PCT

Date: Wednesday, 31 August 2011

Date Author Subject
Wed, 31 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of pBAD

Date: Friday, 02 September 2011

Date Author Subject
Fri, 02 Sep 2011 00:00:00 GMT Sukdong Lee PCR Overlap Extension (OmpA+Silicatein Fusion)
Fri, 02 Sep 2011 00:01:00 GMT Sukdong Lee Gel of PCR Overlap
Fri, 02 Sep 2011 00:02:00 GMT Sukdong Lee PCR Overlap Test

Date: Tuesday, 06 September 2011

Date Author Subject
Tue, 06 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Overlap
Tue, 06 Sep 2011 00:01:00 GMT Sukdong Lee PCR Overlap Extension
Tue, 06 Sep 2011 18:47:17 GMT Viki Ding Further cloning

Date: Wednesday, 07 September 2011

Date Author Subject
Wed, 07 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Products/Gel Extraction

Date: Thursday, 08 September 2011

Date Author Subject
Thu, 08 Sep 2011 00:00:00 GMT Sukdong Lee PCR Overlap Extension
Thu, 08 Sep 2011 00:01:00 GMT Sukdong Lee Gel Extraction of the PCR Overlap Extension
Thu, 08 Sep 2011 00:03:00 GMT Sukdong Lee Transformation of pBAD+ OmpA
Thu, 08 Sep 2011 00:04:00 GMT Sukdong Lee PCR of Silicatein
Thu, 08 Sep 2011 18:51:05 GMT Viki Ding More cloning

Date: Friday, 09 September 2011

Date Author Subject
Fri, 09 Sep 2011 00:00:00 GMT Sukdong Lee Part II of PCR Overlap
Fri, 09 Sep 2011 00:01:00 GMT Sukdong Lee Liquid Cultures of pBAD and pBAD+OmpA
Fri, 09 Sep 2011 00:02:00 GMT Sukdong Lee PCR of Silicatein
Fri, 09 Sep 2011 00:03:00 GMT Sukdong Lee Gel of Part2+3 and Test of Part 3

Date: Saturday, 10 September 2011

Date Author Subject
Sat, 10 Sep 2011 00:00:00 GMT Sukdong Lee Miniprep of OmpA+pBAD and pBAD
Sat, 10 Sep 2011 00:01:00 GMT Sukdong Lee Gel Extraction of Part 3
Sat, 10 Sep 2011 00:03:00 GMT Sukdong Lee Gel of PCR Silicatein
Sat, 10 Sep 2011 00:04:00 GMT Sukdong Lee PCR Overlap Check

Date: Monday, 12 September 2011

Date Author Subject
Mon, 12 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Products
Mon, 12 Sep 2011 00:01:00 GMT Sukdong Lee PCR of Part 3 and Silicatein
Mon, 12 Sep 2011 18:54:16 GMT Viki Ding Verifying clones

Date: Tuesday, 13 September 2011

Date Author Subject
Tue, 13 Sep 2011 00:00:00 GMT Sukdong Lee PCR of Part 3

Date: Wednesday, 14 September 2011

Date Author Subject
Wed, 14 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR

Date: Friday, 16 September 2011

Date Author Subject
Fri, 16 Sep 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein, part 1, part S, part 2, and part 3
Fri, 16 Sep 2011 00:01:00 GMT Sukdong Lee Gel of PCR Products+Extraction
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