From 2011.igem.org
Further cloning
Project: Surface Display
Date: Tue, 06 Sep 2011 18:47:17 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 18:51:01 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
Double digestion
INP-pMCS5BB
- 1 uL XhoI
- 1 uL SpeI
- 3 uL buffer 4
- 0.3 uL BSA
- 24.7 uL plasmid DNA
INP-pMCS5BB
- 1 uL XhoI
- 1 uL PstI
- 3 uL buffer 4
- 0.3 uL BSA
- 24.7 uL plasmid DNA
SiL
- 1 uL XhoI
- 1 uL SpeI
- 3 uL buffer 4
- 0.3 uL BSA
- 24.7 Sil DNA
GFP
- 1 uL XhoI
- 1 uL PstI
- 3 uL buffer 4
- 0.3 uL BSA
- 24.7 GFP DNA
Incubate at 37 degrees overnight
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