From 2011.igem.org
Ligation of OmpA and pBAD
Project: Surface Display
Date: Thu, 09 Jun 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Tue, 23 Aug 2011 12:06:53 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
Combine insert and plasmid.
Procedures:
-Used 7 uL of plasmid and 11 uL of insert for a 20 uL rxn (100 ng).
-A control was made with pBAD (4 uL=50 ng) and water to check for self-ligation.
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