From 2011.igem.org
Primer Design for OmpA
Project: Surface Display
Date: Thu, 07 Apr 2011 11:20:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 22 Aug 2011 09:05:03 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
Designed primers for the E. coli BL21 strain of E. coli to obtain the OmpA gene for the silicatein surface display.
We designed the primers to have the BglII at the beginning. Then the gene followed by a NheI site which ended with a stop codon and a NotI site. The NheI site is so that we can cut the gene out later for the fusion.
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