From 2011.igem.org
PCR of INP
Project: Surface Display
Date: Tue, 28 Jun 2011 16:10:19 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 16:15:50 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
- Mon, 26 Sep 2011 18:06:06 GMT (dingx055): entry data updated by dingx055 (id=9)
Objective: to obtain INP fragments.
50 uL reactions (negative control=no DNA, add H2O to substitute)
1 uL vent pol.
2 uL dNTP
5 uL DNA template
5 uL forward primer
5 uL reverse primer
5 uL vent buffer
27 uL nuclease free water
PCR conditions
95 degrees >> 4 min
95 degrees >> 30 sec
60 degrees >> 30 sec
72 degrees >> 1 min
34 cycles
72 degrees >> 10 min
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