From 2011.igem.org
pucBB Liquid Cultures
Project: Surface Display
Date: Thu, 14 Apr 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 22 Aug 2011 09:15:51 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
To prepare the pucBB plasmid to hold the ompA gene.
Procedure:
A glycerol stock of JM109 strain containing the pucBB+GFP was grown in LB+Amp in a 37 degree shaker overnight.
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