From 2011.igem.org
Silicatein: Digest of pucBB
Project: Surface Display
Date: Wed, 29 Jun 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Thu, 25 Aug 2011 14:42:37 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
- Tue, 27 Sep 2011 17:13:13 GMT (admin): entry data updated by admin (id=1)
Purpose:
Prepare pucBB plasmid for digestion. Silicatein doesn't need to be digested since it was made with the overhangs.
Procedures:
Same, but digest for 2 hours.
Notes:
-Silicatein= 1kb
-pucBB=2.1 kb
-BglII and NotI are the restriction sites.
Revision: Updated 9/27/2011 to include image.
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