From 2011.igem.org
Ligation and Transformation
Project: Surface Display
Date: Mon, 22 Aug 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 29 Aug 2011 13:26:16 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
Redo transformation with previous ligation and continue with a new ligation.
Procedures:
6 uL ligation
-3.5 uL vector
-1.5 uL insert
-0.5 uL buffer
-0.5 uL ligase.
Liquid Cultures and Gogreen PCR
Purpose:
Continue screening for pucBB+Silicatein.
Procedures:
Same
Results:
Negative.
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