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Verifying clones

Project: Surface Display

Date: Mon, 12 Sep 2011 18:54:16 GMT

Author: Viki Ding (dingx055)

Access: Public

Revision History:

• Mon, 26 Sep 2011 18:57:42 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)

Single digestion

- 1 uL BamHI

- 10 uL plasmid DNA

- 3 uL buffer 4

Plasmids

- INP-pMCS5BB

- INP-GFP-pMCS5BB

- INP-Sil-pMCS5BB

Incubate at 37 degrees overnight

Results

- INP pMCS5BB: band at ~5.5 kb

- INP-GFP: band at ~6.2 kb

- INP-Sil: ~6.5 kb

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