"

From 2011.igem.org

Home	Team	Project	Software	Protocols	Notebook	Attributions	Safety

Miniprep and Sequencing of the TOPO Silicatein

Project: Surface Display

Date: Thu, 28 Jul 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

• Mon, 29 Aug 2011 13:13:55 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Extract the TOPO plasmids and send for sequencing.

Procedures:

Same

Results:

-Miniprep

TOPO 1=284.8 ng/ul

TOPO 5=209.1

TOPO 6=227.7

TOPO 7=254.5

-Sequencing

TOPO 1=5 point mutations, 1 major conflict

TOPO 5=X (Bad)

TOPO 6=5 point mutations, 1 major conflict

TOPO 7=3 point mutations, 0 major conflicts

---

240px