From 2011.igem.org
PCR Amplification of OmpA
Project: Surface Display
Date: Thu, 14 Apr 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 22 Aug 2011 09:13:32 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
To replicate the ompA gene and place into a pucBB plasmid.
Procedures:
Followed standard protocol.
For 50 ul reaction add:
-1 uL Template
-1 uL of each primer
-5 uL 10x Thermobuffer
-1 uL 10mM ddNTPs
-2.5 uL 10mM MgSO4
-0.5 uL DNA polymerase:Vent
-38 uL ddH20
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