From 2011.igem.org
Part II of PCR Overlap
Project: Surface Display
Date: Fri, 09 Sep 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Fri, 16 Sep 2011 22:01:56 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
Purpose:
Compute PCR overlap and attempt second overlap extension.
Procedures:
Combine parts at annealing temperature (~53-55 C) for 15 minutes without primers. Then add the primers and run regular PCR cycles.
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