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Cloning #3

Project: Surface Display

Date: Thu, 25 Aug 2011 18:25:40 GMT

Author: Viki Ding (dingx055)

Access: Public

Revision History:

• Mon, 26 Sep 2011 18:30:49 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)

Ligation:

INP 50uL rxn

- vector (4.8 ug/mL): 20 uL

- INP (17.6 ug/mL): 22 uL

- ligase: 1 uL

- ligase buffer: 5 uL

- H2O: 2 uL

Negative control 30 uL rxn

- vector: 6 uL

- H2O: 20 uL

- ligase: 1 uL

- ligase buffer: 3 uL

Incubate at 16 degrees for 1 hr.

Transformation: see protocal 7/13/11

Single digestion of 8/24/11 clones

- 1 uL BamHI

- 3 buffer 4

- 26 uL cloned plasmid DNA

Incubate at 37 degrees overnight

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