From 2011.igem.org
Ligation of pBAD+Silicatein and Digest of TOPO Silicatein
Project: Surface Display
Date: Tue, 23 Aug 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 29 Aug 2011 13:27:09 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
- Mon, 29 Aug 2011 13:29:56 GMT (leex4462): entry data updated by leex4462 (id=3)
- Mon, 29 Aug 2011 13:30:55 GMT (leex4462): entry data updated by leex4462 (id=3)
Purpose:
-Attempt pBADD ligation in case the silicatein is toxic to the E. coli.
-Digest more silicatein since low concentrations
Procedures:
Same, used pBAD from an instructor
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