From 2011.igem.org
TOPO Miniprep and Gogreen PCR and Gel
Project: Surface Display
Date: Fri, 22 Jul 2011 00:00:00 GMT
Author: Sukdong Lee (leex4462)
Access: Public
Revision History:
- Mon, 29 Aug 2011 13:12:01 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
- Tue, 27 Sep 2011 17:19:53 GMT (admin): entry data updated by admin (id=1)
- Tue, 27 Sep 2011 17:20:38 GMT (admin): entry data updated by admin (id=1)
Purpose:
Extract plasmids
Procedures:
Same
Results:
-Miniprep
TOPO 1=485.3 ng/uL
TOPO 2=247.8
TOPO 3=X (strange reading)
TOPO 4=185.9
TOPO 6=366.3
TOPO 7=394.8
TOPO 8=X (strange reading)
TOPO 9=177.6
TOPO 10=322.4
-Gogreen resulted in smeared DNA on the gel.
Revision: Updated 9/27/2011 to include image.
Revision: Updated 9/27/2011 to include a second image.
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