Team:Minnesota/leex4462-06232011000000-65

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Restriction Digests

Project: Surface Display

Date: Thu, 23 Jun 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Tue, 23 Aug 2011 12:11:30 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Digest TOPO OmpA and pBAD plasmid for ligation and transformation.


Procedures:

Digest with NdeI and NotI.


Results:

Tube 1= Fail

Tube 8= 3.3 ng/uL->ligation 1

Tube 10= 3.8-> ligation 2

pBAD=13.5




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