Team:Minnesota/davis950-09102011130503-121

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Miniprep of pUCBB-lacZ and pBBR

Project: Regulatory

Date: Sat, 10 Sep 2011 13:05:03 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Sat, 10 Sep 2011 13:06:53 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Purpose: to isolte the pUCBB-lacZ and pBBR plasmids from overnight cultures.


Procedure: performed a miniprep of 4 3ml overnight cultures according to the Promega Wizard Plus miniprep kit specifications.


Results:

Good concentrations were achieved for both pUCBB-lacZ and pBBR. Minipreps stored in the -20 degree C freezer.



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