From 2011.igem.org
PCR trial #2
Project: Surface Display
Date: Tue, 26 Apr 2011 16:02:53 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 16:05:57 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
50 uL reaction
same volumes as before (4/26)
PCR conditions
For GFP:
95degrees >> 4min
95degrees >> 30 sec
60degrees >> 30 sec
72 degrees >> 45 sec
72 degrees >> 10min
34 cycles
For INP:
95degrees >> 4min
95degrees >> 30 sec
55degrees >> 30 sec
72 degrees >> 45 sec
72 degrees >> 10min
34 cycles
Results: multiple bands, not the correct size.
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