Team:Minnesota/dingx055-04262011160253-154

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PCR trial #2

Project: Surface Display

Date: Tue, 26 Apr 2011 16:02:53 GMT

Author: Viki Ding (dingx055)

Access: Public

Revision History:

  • Mon, 26 Sep 2011 16:05:57 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)

50 uL reaction

same volumes as before (4/26)


PCR conditions


For GFP:

95degrees >> 4min

95degrees >> 30 sec

60degrees >> 30 sec

72 degrees >> 45 sec

72 degrees >> 10min

34 cycles


For INP:

95degrees >> 4min

95degrees >> 30 sec

55degrees >> 30 sec

72 degrees >> 45 sec

72 degrees >> 10min

34 cycles


Results: multiple bands, not the correct size.



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