From 2011.igem.org
Cloning
Project: Surface Display
Date: Sat, 27 Aug 2011 18:17:00 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 18:20:54 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
- Mon, 26 Sep 2011 18:25:34 GMT (dingx055): entry data updated by dingx055 (id=9)
Ligation:
50 uL rxn
- vector (3.16 ug/mL): 20 uL
- INP (11.1 ug/mL): 22 uL
- ligase: 1 uL
- ligase buffer: 5 uL
- H2O: 2 uL
negative control: 30 uL rxn
- vector (3.16 ug/mL): 6 uL
- H2O: 20 uL
- ligase: 1 uL
- ligase buffer: 3 uL
Incubate at 16 degrees for 1 hr.
Transformation: see protocol from 7/13/11
NOTE: forgot to incubate on ice for 30 min.
Results
- positive control: small white colonies
- negative control: scattered big colonies
- INP plate: scattered big colonies
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