Team:Minnesota/dingx055-08272011181700-164

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Cloning

Project: Surface Display

Date: Sat, 27 Aug 2011 18:17:00 GMT

Author: Viki Ding (dingx055)

Access: Public

Revision History:

  • Mon, 26 Sep 2011 18:20:54 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
  • Mon, 26 Sep 2011 18:25:34 GMT (dingx055): entry data updated by dingx055 (id=9)

Ligation:


50 uL rxn

- vector (3.16 ug/mL): 20 uL

- INP (11.1 ug/mL): 22 uL

- ligase: 1 uL

- ligase buffer: 5 uL

- H2O: 2 uL


negative control: 30 uL rxn

- vector (3.16 ug/mL): 6 uL

- H2O: 20 uL

- ligase: 1 uL

- ligase buffer: 3 uL


Incubate at 16 degrees for 1 hr.


Transformation: see protocol from 7/13/11


NOTE: forgot to incubate on ice for 30 min.


Results

- positive control: small white colonies

- negative control: scattered big colonies

- INP plate: scattered big colonies



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