From 2011.igem.org
Digestion of INP
Project: Surface Display
Date: Tue, 12 Jul 2011 16:17:17 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 16:20:00 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
Objective: double digestion, prepare for ligation
30 uL reaction
INP tube
1 uL BamHI
1 uL XhoI
3 uL buffer 4
0.3 uL BSA
24.7 uL INP DNA
pMCS5BB tube
1 uL BamHI
1 uL XhoI
3 uL buffer 4
0.3 uL BSA
24.7 uL plasmid DNA
Incubate at 37 degrees overnight.
|
"
