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Team:Minnesota/Notebook
From 2011.igem.org
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__NOTOC__ | __NOTOC__ | ||
{{Team:Minnesota/Top}} | {{Team:Minnesota/Top}} | ||
| - | <br>=iGEM 2011 Laboratory Notebook= | + | <br> |
| + | =iGEM 2011 Laboratory Notebook= | ||
==Project: Regulatory== | ==Project: Regulatory== | ||
| Line 1,497: | Line 1,498: | ||
| [[Team:Minnesota/leex4462-09162011000100-152|Gel of PCR Products+Extraction]] | | [[Team:Minnesota/leex4462-09162011000100-152|Gel of PCR Products+Extraction]] | ||
|} | |} | ||
| + | |||
{{Team:Minnesota/Bottom}} | {{Team:Minnesota/Bottom}} | ||
Latest revision as of 22:46, 27 September 2011
| Home | Team | Project | Software | Protocols | Notebook | Attributions | Safety |
|---|
| Date | Author | Subject |
|---|---|---|
| Tue, 29 Mar 2011 12:00:00 GMT | Nathan Davis | Transformation of comp. cells with registry parts |
Date: Thursday, 07 April 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 07 Apr 2011 15:14:00 GMT | Nathan Davis | EnvZ mutant comp. cells made |
Date: Monday, 18 April 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 18 Apr 2011 12:00:00 GMT | Nathan Davis | Registry parts and results of X-gal assay of I15008 |
Date: Thursday, 21 April 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 21 Apr 2011 12:00:00 GMT | Nathan Davis | Transformation of pCph8 from Voigt lab |
Date: Thursday, 26 May 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 26 May 2011 12:00:00 GMT | Nathan Davis | PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep |
Date: Monday, 18 July 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 18 Jul 2011 14:59:48 GMT | Nathan Davis | Transformation of comp. cell with registry parts |
| Mon, 18 Jul 2011 15:28:28 GMT | Nathan Davis | Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet |
Date: Wednesday, 20 July 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 20 Jul 2011 16:14:10 GMT | Nathan Davis | Miniprep of pUCbb-pBad and pUCbb-pTet |
Date: Friday, 22 July 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 22 Jul 2011 19:58:49 GMT | Sung Bong Lee | run gel with pcr product of ho1 and cph 8 |
Date: Monday, 25 July 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 25 Jul 2011 14:23:53 GMT | Sung Bong Lee | Gel extraction of PCR product of ho1 and Cph8 |
Date: Thursday, 28 July 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 28 Jul 2011 20:25:02 GMT | Sung Bong Lee | Run gel with NEW PCR product of ho1 and cph8 |
Date: Wednesday, 03 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 03 Aug 2011 16:41:47 GMT | Nathan Davis | Gel extraction of Cph8 and ho1 |
| Wed, 03 Aug 2011 16:44:51 GMT | Nathan Davis | Digest of ho1 and Cph8 |
Date: Wednesday, 10 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 10 Aug 2011 14:59:42 GMT | Nathan Davis | Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8 |
Date: Saturday, 13 August 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 13 Aug 2011 12:35:20 GMT | Nathan Davis | Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1 |
Date: Tuesday, 16 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 16 Aug 2011 13:55:44 GMT | Nathan Davis | Digest of pcyA and ho1 |
Date: Tuesday, 23 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 23 Aug 2011 15:50:51 GMT | Nathan Davis | Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1 |
Date: Tuesday, 30 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 30 Aug 2011 13:22:13 GMT | Nathan Davis | Gel extraction and ligation of pcyA and ho1 |
| Tue, 30 Aug 2011 13:29:13 GMT | Nathan Davis | PCR of ompR and silicatein |
Date: Thursday, 01 September 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 01 Sep 2011 14:14:48 GMT | Nathan Davis | Gel extraction of ompR and silicatein |
| Thu, 01 Sep 2011 14:18:25 GMT | Nathan Davis | Transformation of pcyA/ho1 ligation product |
Date: Friday, 09 September 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 09 Sep 2011 11:45:17 GMT | Nathan Davis | Miniprep of pUC-pcyA-ho1 |
| Fri, 09 Sep 2011 12:13:01 GMT | Nathan Davis | Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8 |
Date: Saturday, 10 September 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 10 Sep 2011 13:02:58 GMT | Nathan Davis | Transformation of pUC-pcyA-ho1-Cph8 |
| Sat, 10 Sep 2011 13:05:03 GMT | Nathan Davis | Miniprep of pUCBB-lacZ and pBBR |
| Sat, 10 Sep 2011 13:06:54 GMT | Nathan Davis | Digest of pUCBB-lacZ, pBBR, and ompR |
Project: Surface Display
Date: Thursday, 07 April 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 07 Apr 2011 11:20:00 GMT | Sukdong Lee | Primer Design for OmpA |
Date: Sunday, 10 April 2011
| Date | Author | Subject |
|---|---|---|
| Sun, 10 Apr 2011 00:00:00 GMT | Sukdong Lee | OmpA BL21 Genomic Liquid Cultures |
Date: Monday, 11 April 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 11 Apr 2011 00:00:00 GMT | Sukdong Lee | OmpA BL21 Genomic Extraction |
Date: Thursday, 14 April 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | PCR Amplification of OmpA |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | pucBB Liquid Cultures |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | Gel Electrophoresis of OmpA PCR Product |
| Thu, 14 Apr 2011 00:00:00 GMT | Sukdong Lee | Gel Purification of OmpA PCR Products |
Date: Friday, 15 April 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 15 Apr 2011 00:00:00 GMT | Sukdong Lee | Miniprep of pucBB+GFP |
| Fri, 15 Apr 2011 00:00:00 GMT | Sukdong Lee | Restriction Digest of pucBB+GFP and Gel extraction of OmpA |
Date: Saturday, 16 April 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 16 Apr 2011 00:01:00 GMT | Sukdong Lee | Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP |
| Sat, 16 Apr 2011 00:02:00 GMT | Sukdong Lee | Restriction Digest of pucBB and ompA inserts |
| Sat, 16 Apr 2011 00:03:00 GMT | Sukdong Lee | Gel Electrophoresis of OmpA |
Date: Tuesday, 19 April 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 19 Apr 2011 00:00:00 GMT | Sukdong Lee | PCR Amplification of Genomic BL21 |
| Tue, 19 Apr 2011 00:01:00 GMT | Sukdong Lee | Gel Extraction of OmpA |
Date: Wednesday, 20 April 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 20 Apr 2011 00:00:00 GMT | Sukdong Lee | pBAD plasmid liquid cultures |
Date: Thursday, 21 April 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 21 Apr 2011 00:00:00 GMT | Sukdong Lee | Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction |
| Thu, 21 Apr 2011 12:00:00 GMT | Nathan Davis | Gel extraction of Silicatein alpha DNA |
Date: Friday, 22 April 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 22 Apr 2011 00:00:00 GMT | Sukdong Lee | Miniprep, Digest, and Extract |
Date: Saturday, 23 April 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 23 Apr 2011 00:00:00 GMT | Sukdong Lee | Ligation of pBAD and OmpA |
Date: Tuesday, 26 April 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 26 Apr 2011 00:00:00 GMT | Sukdong Lee | Restriction digest of OmpA, PCR of OmpA (back-up) |
| Tue, 26 Apr 2011 15:52:22 GMT | Viki Ding | PCR set up |
| Tue, 26 Apr 2011 16:02:53 GMT | Viki Ding | PCR trial #2 |
Date: Thursday, 28 April 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 28 Apr 2011 00:00:00 GMT | Sukdong Lee | PCR of OmpA and Gel |
Date: Saturday, 30 April 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 30 Apr 2011 00:00:00 GMT | Sukdong Lee | Speed Vacuum |
Date: Tuesday, 03 May 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 03 May 2011 00:00:00 GMT | Sukdong Lee | Restriction Digest of pBAD and OmpA insert |
Date: Wednesday, 04 May 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 04 May 2011 00:00:00 GMT | Sukdong Lee | PCR purification of OmpA and Gel Extraction |
Date: Thursday, 05 May 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 05 May 2011 00:00:00 GMT | Sukdong Lee | Ligation of OmpA and pBAD |
Date: Friday, 06 May 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 06 May 2011 00:00:00 GMT | Sukdong Lee | Transformation |
Date: Monday, 23 May 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 23 May 2011 00:00:00 GMT | Sukdong Lee | Transformation and Liquid cultures of OmpA transformants |
Date: Tuesday, 24 May 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 24 May 2011 00:00:00 GMT | Sukdong Lee | Miniprep and PCR, Liquid Cultures |
Date: Wednesday, 25 May 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 25 May 2011 00:00:00 GMT | Sukdong Lee | Gel of OmpA Plasmid |
Date: Wednesday, 01 June 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 01 Jun 2011 00:00:00 GMT | Sukdong Lee | Liquid cultures of pBAD plasmid |
Date: Thursday, 02 June 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 02 Jun 2011 00:00:00 GMT | Sukdong Lee | PCR Amplification of OmpA and Miniprep of pBADBB |
Date: Friday, 03 June 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 03 Jun 2011 00:00:00 GMT | Sukdong Lee | Gel Electrophoresis of OmpA and pBAD PCR Products and Digests |
| Fri, 03 Jun 2011 00:01:00 GMT | Sukdong Lee | Extractions of OmpA |
Date: Sunday, 05 June 2011
| Date | Author | Subject |
|---|---|---|
| Sun, 05 Jun 2011 00:00:00 GMT | Sukdong Lee | Speed Vac and Digests |
Date: Monday, 06 June 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 06 Jun 2011 00:00:00 GMT | Sukdong Lee | Gel and Extraction |
Date: Tuesday, 07 June 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 07 Jun 2011 00:00:00 GMT | Sukdong Lee | Digestion of OmpA |
Date: Wednesday, 08 June 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 08 Jun 2011 00:00:00 GMT | Sukdong Lee | PCR Purification/Gel Extraction |
Date: Thursday, 09 June 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 09 Jun 2011 00:00:00 GMT | Sukdong Lee | Ligation of OmpA and pBAD |
Date: Friday, 10 June 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 10 Jun 2011 00:00:00 GMT | Sukdong Lee | Transformation of OmpA plasmid |
Date: Sunday, 12 June 2011
| Date | Author | Subject |
|---|---|---|
| Sun, 12 Jun 2011 00:00:00 GMT | Sukdong Lee | Electroporation transformation |
Date: Monday, 13 June 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 13 Jun 2011 00:00:00 GMT | Sukdong Lee | Ligation of OmpA+pBAD |
Date: Tuesday, 14 June 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 14 Jun 2011 00:00:00 GMT | Sukdong Lee | Ligation |
Date: Wednesday, 15 June 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 15 Jun 2011 00:00:00 GMT | Sukdong Lee | Electroporation Transformation |
Date: Saturday, 18 June 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 18 Jun 2011 00:00:00 GMT | Sukdong Lee | PCR of OmpA |
Date: Monday, 20 June 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 20 Jun 2011 00:00:00 GMT | Sukdong Lee | TOPO Cloning and Gel Extraction |
Date: Tuesday, 21 June 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 21 Jun 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures of TOPO Cloning |
Date: Wednesday, 22 June 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 22 Jun 2011 00:00:00 GMT | Sukdong Lee | GoGreen PCR and Miniprep of TOPO Liquid Cultures |
Date: Thursday, 23 June 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 23 Jun 2011 00:00:00 GMT | Sukdong Lee | Restriction Digests |
Date: Friday, 24 June 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 24 Jun 2011 00:00:00 GMT | Sukdong Lee | Gel Extraction and Ligation |
Date: Saturday, 25 June 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 25 Jun 2011 00:00:00 GMT | Sukdong Lee | Electroporation |
| Sat, 25 Jun 2011 00:01:00 GMT | Sukdong Lee | Chemical Transformation |
Date: Sunday, 26 June 2011
| Date | Author | Subject |
|---|---|---|
| Sun, 26 Jun 2011 00:00:00 GMT | Sukdong Lee | Liquid cultures of OmpA plasmids |
Date: Monday, 27 June 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 27 Jun 2011 00:00:00 GMT | Sukdong Lee | Miniprep of OmpA and pBAD plasmid |
Date: Tuesday, 28 June 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 28 Jun 2011 00:00:00 GMT | Sukdong Lee | Gogreen PCR and Digestion Check |
| Tue, 28 Jun 2011 16:10:19 GMT | Viki Ding | PCR of INP |
Date: Wednesday, 29 June 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 29 Jun 2011 00:00:00 GMT | Sukdong Lee | Silicatein: Digest of pucBB |
| Wed, 29 Jun 2011 00:01:00 GMT | Sukdong Lee | Ligation of pucBB plasmid and Silicatein |
Date: Thursday, 30 June 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 30 Jun 2011 00:00:00 GMT | Sukdong Lee | Transformation |
Date: Friday, 01 July 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 01 Jul 2011 00:00:00 GMT | Sukdong Lee | Liquid cultures of pucBB+Silicatein Transformants |
Date: Saturday, 02 July 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 02 Jul 2011 00:00:00 GMT | Sukdong Lee | Miniprep of Silicatein+pucBB transformants |
Date: Monday, 04 July 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 04 Jul 2011 00:00:00 GMT | Sukdong Lee | Liquid Gogreen PCR |
Date: Wednesday, 06 July 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 06 Jul 2011 16:15:53 GMT | Viki Ding | INP PCR clean up |
Date: Thursday, 07 July 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 07 Jul 2011 00:00:00 GMT | Sukdong Lee | Gogreen of Silicatein pucBB plasmid |
| Thu, 07 Jul 2011 00:01:00 GMT | Sukdong Lee | Restriction Digest of pucBB and PCR of Silicatein |
Date: Friday, 08 July 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 08 Jul 2011 00:00:00 GMT | Sukdong Lee | Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation |
Date: Monday, 11 July 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 11 Jul 2011 00:00:00 GMT | Sukdong Lee | Gel Extraction of Silicatein and Liquid Cultures of pucBB |
Date: Tuesday, 12 July 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 12 Jul 2011 00:00:00 GMT | Sukdong Lee | Transformation of pucBB+Silicatein |
| Tue, 12 Jul 2011 16:17:17 GMT | Viki Ding | Digestion of INP |
Date: Wednesday, 13 July 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 13 Jul 2011 00:00:00 GMT | Sukdong Lee | Gogreen PCR and Miniprep |
| Wed, 13 Jul 2011 16:20:04 GMT | Viki Ding | INP cloning |
Date: Friday, 15 July 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 15 Jul 2011 00:00:00 GMT | Sukdong Lee | pucBB Digest and pucBB Liquid Cultures |
Date: Saturday, 16 July 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 16 Jul 2011 00:00:00 GMT | Sukdong Lee | Miniprep of pucBB and gel extraction of pucBB |
Date: Wednesday, 20 July 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 20 Jul 2011 00:00:00 GMT | Sukdong Lee | PCR of Silicatein, Gel Extraction, and TOPO Cloning |
| Wed, 20 Jul 2011 00:01:00 GMT | Sukdong Lee | TOPO Transformants and Liquid Cultures |
| Wed, 20 Jul 2011 17:59:22 GMT | Viki Ding | Transformation trial #2 |
Date: Friday, 22 July 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 22 Jul 2011 00:00:00 GMT | Sukdong Lee | TOPO Miniprep and Gogreen PCR and Gel |
Date: Monday, 25 July 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 25 Jul 2011 00:00:00 GMT | Sukdong Lee | Gogreen PCR X3 and Gel X3 |
Date: Tuesday, 26 July 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 26 Jul 2011 00:00:00 GMT | Sukdong Lee | PCR of Silicatein and TOPO |
| Tue, 26 Jul 2011 18:02:01 GMT | Viki Ding | INP PCR with Taq mix |
Date: Wednesday, 27 July 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 27 Jul 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures and Colony Gogreen PCR |
| Wed, 27 Jul 2011 18:06:41 GMT | Viki Ding | INP PCR w/Taq & digestion |
Date: Thursday, 28 July 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 28 Jul 2011 00:00:00 GMT | Sukdong Lee | Miniprep and Sequencing of the TOPO Silicatein |
Date: Tuesday, 02 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 02 Aug 2011 18:12:35 GMT | Viki Ding | Ligation & transformation |
Date: Wednesday, 03 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 03 Aug 2011 00:00:00 GMT | Sukdong Lee | Digestion of Silicatein TOPO 7 |
Date: Thursday, 04 August 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 04 Aug 2011 00:00:00 GMT | Sukdong Lee | Gel Extraction, Ligation, and Transformation |
Date: Monday, 08 August 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 08 Aug 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures, Master Plate, Gogreen, and Gel |
Date: Tuesday, 09 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 09 Aug 2011 00:00:00 GMT | Sukdong Lee | Miniprep, Gogreen Liquid PCR, and Gel |
Date: Wednesday, 10 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 10 Aug 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures, Liquid Gogreen PCR |
Date: Thursday, 11 August 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 11 Aug 2011 00:00:00 GMT | Sukdong Lee | More Liquid Cultures, Liquid Gogreen PCR |
Date: Friday, 12 August 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 12 Aug 2011 00:00:00 GMT | Sukdong Lee | Transformation of TOPO Silicatein Back-up |
Date: Saturday, 13 August 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 13 Aug 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures of Back-Up Silicatein TOPO |
Date: Sunday, 14 August 2011
| Date | Author | Subject |
|---|---|---|
| Sun, 14 Aug 2011 00:00:00 GMT | Sukdong Lee | Miniprep of Silicatein TOPO |
Date: Monday, 15 August 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 15 Aug 2011 00:00:00 GMT | Sukdong Lee | Digest of pucBB and Silicatein TOPO |
Date: Tuesday, 16 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 16 Aug 2011 00:00:00 GMT | Sukdong Lee | Miniprep of pucBB and Gel Extraction |
| Tue, 16 Aug 2011 18:14:41 GMT | Viki Ding | Digestion |
Date: Wednesday, 17 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 17 Aug 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures of Transformants and Gogreen |
Date: Thursday, 18 August 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 18 Aug 2011 00:00:00 GMT | Sukdong Lee | Gogreen PCR of Liquid Cultures |
Date: Friday, 19 August 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 19 Aug 2011 00:00:00 GMT | Sukdong Lee | Gogreen PCR of Liquid Cultures |
Date: Monday, 22 August 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 22 Aug 2011 00:00:00 GMT | Sukdong Lee | Ligation and Transformation |
| Mon, 22 Aug 2011 10:15:36 GMT | Sukdong Lee | Minnesota/leex4462-08222011101536-34 |
Date: Tuesday, 23 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 23 Aug 2011 00:00:00 GMT | Sukdong Lee | Ligation of pBAD+Silicatein and Digest of TOPO Silicatein |
| Tue, 23 Aug 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures, Gogreen PCR of Silicatein+pBAD |
Date: Wednesday, 24 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 24 Aug 2011 00:00:00 GMT | Sukdong Lee | pBAD+Silicatein transformation and gel extraction of TOPO Silicatein |
| Wed, 24 Aug 2011 00:00:00 GMT | Sukdong Lee | Ligation, Digest, Transformation of pBAD |
Date: Thursday, 25 August 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 25 Aug 2011 00:00:00 GMT | Sukdong Lee | Liquid Cultures, Gogreen PCR of Silicatein+pBAD |
| Thu, 25 Aug 2011 00:00:00 GMT | Sukdong Lee | Minnesota/leex4462-08252011000000-124 |
| Thu, 25 Aug 2011 18:25:40 GMT | Viki Ding | Cloning #3 |
Date: Friday, 26 August 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 26 Aug 2011 00:00:00 GMT | Sukdong Lee | Miniprep and Gogreen Check |
| Fri, 26 Aug 2011 00:00:00 GMT | Sukdong Lee | Minnesota/leex4462-08262011000000-125 |
Date: Saturday, 27 August 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 27 Aug 2011 18:17:00 GMT | Viki Ding | Cloning |
Date: Monday, 29 August 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 29 Aug 2011 00:00:00 GMT | Sukdong Lee | Ligation, Digest, Transformation of pBAD |
| Mon, 29 Aug 2011 18:30:52 GMT | Viki Ding | Verifying clones |
Date: Tuesday, 30 August 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 30 Aug 2011 00:00:00 GMT | Sukdong Lee | Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation |
| Tue, 30 Aug 2011 18:38:12 GMT | Viki Ding | SiL & GFP PCT |
Date: Wednesday, 31 August 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 31 Aug 2011 00:00:00 GMT | Sukdong Lee | Miniprep of pBAD |
Date: Friday, 02 September 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 02 Sep 2011 00:00:00 GMT | Sukdong Lee | PCR Overlap Extension (OmpA+Silicatein Fusion) |
| Fri, 02 Sep 2011 00:01:00 GMT | Sukdong Lee | Gel of PCR Overlap |
| Fri, 02 Sep 2011 00:02:00 GMT | Sukdong Lee | PCR Overlap Test |
Date: Tuesday, 06 September 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 06 Sep 2011 00:00:00 GMT | Sukdong Lee | Gel of PCR Overlap |
| Tue, 06 Sep 2011 00:01:00 GMT | Sukdong Lee | PCR Overlap Extension |
| Tue, 06 Sep 2011 18:47:17 GMT | Viki Ding | Further cloning |
Date: Wednesday, 07 September 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 07 Sep 2011 00:00:00 GMT | Sukdong Lee | Gel of PCR Products/Gel Extraction |
Date: Thursday, 08 September 2011
| Date | Author | Subject |
|---|---|---|
| Thu, 08 Sep 2011 00:00:00 GMT | Sukdong Lee | PCR Overlap Extension |
| Thu, 08 Sep 2011 00:01:00 GMT | Sukdong Lee | Gel Extraction of the PCR Overlap Extension |
| Thu, 08 Sep 2011 00:03:00 GMT | Sukdong Lee | Transformation of pBAD+ OmpA |
| Thu, 08 Sep 2011 00:04:00 GMT | Sukdong Lee | PCR of Silicatein |
| Thu, 08 Sep 2011 18:51:05 GMT | Viki Ding | More cloning |
Date: Friday, 09 September 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 09 Sep 2011 00:00:00 GMT | Sukdong Lee | Part II of PCR Overlap |
| Fri, 09 Sep 2011 00:01:00 GMT | Sukdong Lee | Liquid Cultures of pBAD and pBAD+OmpA |
| Fri, 09 Sep 2011 00:02:00 GMT | Sukdong Lee | PCR of Silicatein |
| Fri, 09 Sep 2011 00:03:00 GMT | Sukdong Lee | Gel of Part2+3 and Test of Part 3 |
Date: Saturday, 10 September 2011
| Date | Author | Subject |
|---|---|---|
| Sat, 10 Sep 2011 00:00:00 GMT | Sukdong Lee | Miniprep of OmpA+pBAD and pBAD |
| Sat, 10 Sep 2011 00:01:00 GMT | Sukdong Lee | Gel Extraction of Part 3 |
| Sat, 10 Sep 2011 00:03:00 GMT | Sukdong Lee | Gel of PCR Silicatein |
| Sat, 10 Sep 2011 00:04:00 GMT | Sukdong Lee | PCR Overlap Check |
Date: Monday, 12 September 2011
| Date | Author | Subject |
|---|---|---|
| Mon, 12 Sep 2011 00:00:00 GMT | Sukdong Lee | Gel of PCR Products |
| Mon, 12 Sep 2011 00:01:00 GMT | Sukdong Lee | PCR of Part 3 and Silicatein |
| Mon, 12 Sep 2011 18:54:16 GMT | Viki Ding | Verifying clones |
Date: Tuesday, 13 September 2011
| Date | Author | Subject |
|---|---|---|
| Tue, 13 Sep 2011 00:00:00 GMT | Sukdong Lee | PCR of Part 3 |
Date: Wednesday, 14 September 2011
| Date | Author | Subject |
|---|---|---|
| Wed, 14 Sep 2011 00:00:00 GMT | Sukdong Lee | Gel of PCR |
Date: Friday, 16 September 2011
| Date | Author | Subject |
|---|---|---|
| Fri, 16 Sep 2011 00:00:00 GMT | Sukdong Lee | PCR of Silicatein, part 1, part S, part 2, and part 3 |
| Fri, 16 Sep 2011 00:01:00 GMT | Sukdong Lee | Gel of PCR Products+Extraction |
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