Team:Minnesota/Notebook

From 2011.igem.org

(Difference between revisions)
m (Automatic page editing)
m (Automatic page editing)
 
Line 1: Line 1:
__NOTOC__
__NOTOC__
{{Team:Minnesota/Top}}
{{Team:Minnesota/Top}}
-
<br>=iGEM 2011 Laboratory Notebook=
+
<br>
 +
=iGEM 2011 Laboratory Notebook=
==Project: Regulatory==
==Project: Regulatory==
Line 1,497: Line 1,498:
| [[Team:Minnesota/leex4462-09162011000100-152|Gel of PCR Products+Extraction]]
| [[Team:Minnesota/leex4462-09162011000100-152|Gel of PCR Products+Extraction]]
|}
|}
 +
{{Team:Minnesota/Bottom}}
{{Team:Minnesota/Bottom}}

Latest revision as of 22:46, 27 September 2011

Mnlogo.jpg
Home Team Project Software Protocols Notebook Attributions Safety

iGEM 2011 Laboratory Notebook

Project: Regulatory

Date: Tuesday, 29 March 2011

Date Author Subject
Tue, 29 Mar 2011 12:00:00 GMT Nathan Davis Transformation of comp. cells with registry parts

Date: Thursday, 07 April 2011

Date Author Subject
Thu, 07 Apr 2011 15:14:00 GMT Nathan Davis EnvZ mutant comp. cells made

Date: Monday, 18 April 2011

Date Author Subject
Mon, 18 Apr 2011 12:00:00 GMT Nathan Davis Registry parts and results of X-gal assay of I15008

Date: Thursday, 21 April 2011

Date Author Subject
Thu, 21 Apr 2011 12:00:00 GMT Nathan Davis Transformation of pCph8 from Voigt lab

Date: Thursday, 26 May 2011

Date Author Subject
Thu, 26 May 2011 12:00:00 GMT Nathan Davis PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep

Date: Monday, 18 July 2011

Date Author Subject
Mon, 18 Jul 2011 14:59:48 GMT Nathan Davis Transformation of comp. cell with registry parts
Mon, 18 Jul 2011 15:28:28 GMT Nathan Davis Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet

Date: Wednesday, 20 July 2011

Date Author Subject
Wed, 20 Jul 2011 16:14:10 GMT Nathan Davis Miniprep of pUCbb-pBad and pUCbb-pTet

Date: Friday, 22 July 2011

Date Author Subject
Fri, 22 Jul 2011 19:58:49 GMT Sung Bong Lee run gel with pcr product of ho1 and cph 8

Date: Monday, 25 July 2011

Date Author Subject
Mon, 25 Jul 2011 14:23:53 GMT Sung Bong Lee Gel extraction of PCR product of ho1 and Cph8

Date: Thursday, 28 July 2011

Date Author Subject
Thu, 28 Jul 2011 20:25:02 GMT Sung Bong Lee Run gel with NEW PCR product of ho1 and cph8

Date: Wednesday, 03 August 2011

Date Author Subject
Wed, 03 Aug 2011 16:41:47 GMT Nathan Davis Gel extraction of Cph8 and ho1
Wed, 03 Aug 2011 16:44:51 GMT Nathan Davis Digest of ho1 and Cph8

Date: Wednesday, 10 August 2011

Date Author Subject
Wed, 10 Aug 2011 14:59:42 GMT Nathan Davis Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8

Date: Saturday, 13 August 2011

Date Author Subject
Sat, 13 Aug 2011 12:35:20 GMT Nathan Davis Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1

Date: Tuesday, 16 August 2011

Date Author Subject
Tue, 16 Aug 2011 13:55:44 GMT Nathan Davis Digest of pcyA and ho1

Date: Tuesday, 23 August 2011

Date Author Subject
Tue, 23 Aug 2011 15:50:51 GMT Nathan Davis Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1

Date: Tuesday, 30 August 2011

Date Author Subject
Tue, 30 Aug 2011 13:22:13 GMT Nathan Davis Gel extraction and ligation of pcyA and ho1
Tue, 30 Aug 2011 13:29:13 GMT Nathan Davis PCR of ompR and silicatein

Date: Thursday, 01 September 2011

Date Author Subject
Thu, 01 Sep 2011 14:14:48 GMT Nathan Davis Gel extraction of ompR and silicatein
Thu, 01 Sep 2011 14:18:25 GMT Nathan Davis Transformation of pcyA/ho1 ligation product

Date: Friday, 09 September 2011

Date Author Subject
Fri, 09 Sep 2011 11:45:17 GMT Nathan Davis Miniprep of pUC-pcyA-ho1
Fri, 09 Sep 2011 12:13:01 GMT Nathan Davis Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8

Date: Saturday, 10 September 2011

Date Author Subject
Sat, 10 Sep 2011 13:02:58 GMT Nathan Davis Transformation of pUC-pcyA-ho1-Cph8
Sat, 10 Sep 2011 13:05:03 GMT Nathan Davis Miniprep of pUCBB-lacZ and pBBR
Sat, 10 Sep 2011 13:06:54 GMT Nathan Davis Digest of pUCBB-lacZ, pBBR, and ompR

Project: Surface Display

Date: Thursday, 07 April 2011

Date Author Subject
Thu, 07 Apr 2011 11:20:00 GMT Sukdong Lee Primer Design for OmpA

Date: Sunday, 10 April 2011

Date Author Subject
Sun, 10 Apr 2011 00:00:00 GMT Sukdong Lee OmpA BL21 Genomic Liquid Cultures

Date: Monday, 11 April 2011

Date Author Subject
Mon, 11 Apr 2011 00:00:00 GMT Sukdong Lee OmpA BL21 Genomic Extraction

Date: Thursday, 14 April 2011

Date Author Subject
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee PCR Amplification of OmpA
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee pucBB Liquid Cultures
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee Gel Electrophoresis of OmpA PCR Product
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee Gel Purification of OmpA PCR Products

Date: Friday, 15 April 2011

Date Author Subject
Fri, 15 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB+GFP
Fri, 15 Apr 2011 00:00:00 GMT Sukdong Lee Restriction Digest of pucBB+GFP and Gel extraction of OmpA

Date: Saturday, 16 April 2011

Date Author Subject
Sat, 16 Apr 2011 00:01:00 GMT Sukdong Lee Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP
Sat, 16 Apr 2011 00:02:00 GMT Sukdong Lee Restriction Digest of pucBB and ompA inserts
Sat, 16 Apr 2011 00:03:00 GMT Sukdong Lee Gel Electrophoresis of OmpA

Date: Tuesday, 19 April 2011

Date Author Subject
Tue, 19 Apr 2011 00:00:00 GMT Sukdong Lee PCR Amplification of Genomic BL21
Tue, 19 Apr 2011 00:01:00 GMT Sukdong Lee Gel Extraction of OmpA

Date: Wednesday, 20 April 2011

Date Author Subject
Wed, 20 Apr 2011 00:00:00 GMT Sukdong Lee pBAD plasmid liquid cultures

Date: Thursday, 21 April 2011

Date Author Subject
Thu, 21 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction
Thu, 21 Apr 2011 12:00:00 GMT Nathan Davis Gel extraction of Silicatein alpha DNA

Date: Friday, 22 April 2011

Date Author Subject
Fri, 22 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep, Digest, and Extract

Date: Saturday, 23 April 2011

Date Author Subject
Sat, 23 Apr 2011 00:00:00 GMT Sukdong Lee Ligation of pBAD and OmpA

Date: Tuesday, 26 April 2011

Date Author Subject
Tue, 26 Apr 2011 00:00:00 GMT Sukdong Lee Restriction digest of OmpA, PCR of OmpA (back-up)
Tue, 26 Apr 2011 15:52:22 GMT Viki Ding PCR set up
Tue, 26 Apr 2011 16:02:53 GMT Viki Ding PCR trial #2

Date: Thursday, 28 April 2011

Date Author Subject
Thu, 28 Apr 2011 00:00:00 GMT Sukdong Lee PCR of OmpA and Gel

Date: Saturday, 30 April 2011

Date Author Subject
Sat, 30 Apr 2011 00:00:00 GMT Sukdong Lee Speed Vacuum

Date: Tuesday, 03 May 2011

Date Author Subject
Tue, 03 May 2011 00:00:00 GMT Sukdong Lee Restriction Digest of pBAD and OmpA insert

Date: Wednesday, 04 May 2011

Date Author Subject
Wed, 04 May 2011 00:00:00 GMT Sukdong Lee PCR purification of OmpA and Gel Extraction

Date: Thursday, 05 May 2011

Date Author Subject
Thu, 05 May 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA and pBAD

Date: Friday, 06 May 2011

Date Author Subject
Fri, 06 May 2011 00:00:00 GMT Sukdong Lee Transformation

Date: Monday, 23 May 2011

Date Author Subject
Mon, 23 May 2011 00:00:00 GMT Sukdong Lee Transformation and Liquid cultures of OmpA transformants

Date: Tuesday, 24 May 2011

Date Author Subject
Tue, 24 May 2011 00:00:00 GMT Sukdong Lee Miniprep and PCR, Liquid Cultures

Date: Wednesday, 25 May 2011

Date Author Subject
Wed, 25 May 2011 00:00:00 GMT Sukdong Lee Gel of OmpA Plasmid

Date: Wednesday, 01 June 2011

Date Author Subject
Wed, 01 Jun 2011 00:00:00 GMT Sukdong Lee Liquid cultures of pBAD plasmid

Date: Thursday, 02 June 2011

Date Author Subject
Thu, 02 Jun 2011 00:00:00 GMT Sukdong Lee PCR Amplification of OmpA and Miniprep of pBADBB

Date: Friday, 03 June 2011

Date Author Subject
Fri, 03 Jun 2011 00:00:00 GMT Sukdong Lee Gel Electrophoresis of OmpA and pBAD PCR Products and Digests
Fri, 03 Jun 2011 00:01:00 GMT Sukdong Lee Extractions of OmpA

Date: Sunday, 05 June 2011

Date Author Subject
Sun, 05 Jun 2011 00:00:00 GMT Sukdong Lee Speed Vac and Digests

Date: Monday, 06 June 2011

Date Author Subject
Mon, 06 Jun 2011 00:00:00 GMT Sukdong Lee Gel and Extraction

Date: Tuesday, 07 June 2011

Date Author Subject
Tue, 07 Jun 2011 00:00:00 GMT Sukdong Lee Digestion of OmpA

Date: Wednesday, 08 June 2011

Date Author Subject
Wed, 08 Jun 2011 00:00:00 GMT Sukdong Lee PCR Purification/Gel Extraction

Date: Thursday, 09 June 2011

Date Author Subject
Thu, 09 Jun 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA and pBAD

Date: Friday, 10 June 2011

Date Author Subject
Fri, 10 Jun 2011 00:00:00 GMT Sukdong Lee Transformation of OmpA plasmid

Date: Sunday, 12 June 2011

Date Author Subject
Sun, 12 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation transformation

Date: Monday, 13 June 2011

Date Author Subject
Mon, 13 Jun 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA+pBAD

Date: Tuesday, 14 June 2011

Date Author Subject
Tue, 14 Jun 2011 00:00:00 GMT Sukdong Lee Ligation

Date: Wednesday, 15 June 2011

Date Author Subject
Wed, 15 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation Transformation

Date: Saturday, 18 June 2011

Date Author Subject
Sat, 18 Jun 2011 00:00:00 GMT Sukdong Lee PCR of OmpA

Date: Monday, 20 June 2011

Date Author Subject
Mon, 20 Jun 2011 00:00:00 GMT Sukdong Lee TOPO Cloning and Gel Extraction

Date: Tuesday, 21 June 2011

Date Author Subject
Tue, 21 Jun 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of TOPO Cloning

Date: Wednesday, 22 June 2011

Date Author Subject
Wed, 22 Jun 2011 00:00:00 GMT Sukdong Lee GoGreen PCR and Miniprep of TOPO Liquid Cultures

Date: Thursday, 23 June 2011

Date Author Subject
Thu, 23 Jun 2011 00:00:00 GMT Sukdong Lee Restriction Digests

Date: Friday, 24 June 2011

Date Author Subject
Fri, 24 Jun 2011 00:00:00 GMT Sukdong Lee Gel Extraction and Ligation

Date: Saturday, 25 June 2011

Date Author Subject
Sat, 25 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation
Sat, 25 Jun 2011 00:01:00 GMT Sukdong Lee Chemical Transformation

Date: Sunday, 26 June 2011

Date Author Subject
Sun, 26 Jun 2011 00:00:00 GMT Sukdong Lee Liquid cultures of OmpA plasmids

Date: Monday, 27 June 2011

Date Author Subject
Mon, 27 Jun 2011 00:00:00 GMT Sukdong Lee Miniprep of OmpA and pBAD plasmid

Date: Tuesday, 28 June 2011

Date Author Subject
Tue, 28 Jun 2011 00:00:00 GMT Sukdong Lee Gogreen PCR and Digestion Check
Tue, 28 Jun 2011 16:10:19 GMT Viki Ding PCR of INP

Date: Wednesday, 29 June 2011

Date Author Subject
Wed, 29 Jun 2011 00:00:00 GMT Sukdong Lee Silicatein: Digest of pucBB
Wed, 29 Jun 2011 00:01:00 GMT Sukdong Lee Ligation of pucBB plasmid and Silicatein

Date: Thursday, 30 June 2011

Date Author Subject
Thu, 30 Jun 2011 00:00:00 GMT Sukdong Lee Transformation

Date: Friday, 01 July 2011

Date Author Subject
Fri, 01 Jul 2011 00:00:00 GMT Sukdong Lee Liquid cultures of pucBB+Silicatein Transformants

Date: Saturday, 02 July 2011

Date Author Subject
Sat, 02 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep of Silicatein+pucBB transformants

Date: Monday, 04 July 2011

Date Author Subject
Mon, 04 Jul 2011 00:00:00 GMT Sukdong Lee Liquid Gogreen PCR

Date: Wednesday, 06 July 2011

Date Author Subject
Wed, 06 Jul 2011 16:15:53 GMT Viki Ding INP PCR clean up

Date: Thursday, 07 July 2011

Date Author Subject
Thu, 07 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen of Silicatein pucBB plasmid
Thu, 07 Jul 2011 00:01:00 GMT Sukdong Lee Restriction Digest of pucBB and PCR of Silicatein

Date: Friday, 08 July 2011

Date Author Subject
Fri, 08 Jul 2011 00:00:00 GMT Sukdong Lee Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation

Date: Monday, 11 July 2011

Date Author Subject
Mon, 11 Jul 2011 00:00:00 GMT Sukdong Lee Gel Extraction of Silicatein and Liquid Cultures of pucBB

Date: Tuesday, 12 July 2011

Date Author Subject
Tue, 12 Jul 2011 00:00:00 GMT Sukdong Lee Transformation of pucBB+Silicatein
Tue, 12 Jul 2011 16:17:17 GMT Viki Ding Digestion of INP

Date: Wednesday, 13 July 2011

Date Author Subject
Wed, 13 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen PCR and Miniprep
Wed, 13 Jul 2011 16:20:04 GMT Viki Ding INP cloning

Date: Friday, 15 July 2011

Date Author Subject
Fri, 15 Jul 2011 00:00:00 GMT Sukdong Lee pucBB Digest and pucBB Liquid Cultures

Date: Saturday, 16 July 2011

Date Author Subject
Sat, 16 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB and gel extraction of pucBB

Date: Wednesday, 20 July 2011

Date Author Subject
Wed, 20 Jul 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein, Gel Extraction, and TOPO Cloning
Wed, 20 Jul 2011 00:01:00 GMT Sukdong Lee TOPO Transformants and Liquid Cultures
Wed, 20 Jul 2011 17:59:22 GMT Viki Ding Transformation trial #2

Date: Friday, 22 July 2011

Date Author Subject
Fri, 22 Jul 2011 00:00:00 GMT Sukdong Lee TOPO Miniprep and Gogreen PCR and Gel

Date: Monday, 25 July 2011

Date Author Subject
Mon, 25 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen PCR X3 and Gel X3

Date: Tuesday, 26 July 2011

Date Author Subject
Tue, 26 Jul 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein and TOPO
Tue, 26 Jul 2011 18:02:01 GMT Viki Ding INP PCR with Taq mix

Date: Wednesday, 27 July 2011

Date Author Subject
Wed, 27 Jul 2011 00:00:00 GMT Sukdong Lee Liquid Cultures and Colony Gogreen PCR
Wed, 27 Jul 2011 18:06:41 GMT Viki Ding INP PCR w/Taq & digestion

Date: Thursday, 28 July 2011

Date Author Subject
Thu, 28 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep and Sequencing of the TOPO Silicatein

Date: Tuesday, 02 August 2011

Date Author Subject
Tue, 02 Aug 2011 18:12:35 GMT Viki Ding Ligation & transformation

Date: Wednesday, 03 August 2011

Date Author Subject
Wed, 03 Aug 2011 00:00:00 GMT Sukdong Lee Digestion of Silicatein TOPO 7

Date: Thursday, 04 August 2011

Date Author Subject
Thu, 04 Aug 2011 00:00:00 GMT Sukdong Lee Gel Extraction, Ligation, and Transformation

Date: Monday, 08 August 2011

Date Author Subject
Mon, 08 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Master Plate, Gogreen, and Gel

Date: Tuesday, 09 August 2011

Date Author Subject
Tue, 09 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep, Gogreen Liquid PCR, and Gel

Date: Wednesday, 10 August 2011

Date Author Subject
Wed, 10 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Liquid Gogreen PCR

Date: Thursday, 11 August 2011

Date Author Subject
Thu, 11 Aug 2011 00:00:00 GMT Sukdong Lee More Liquid Cultures, Liquid Gogreen PCR

Date: Friday, 12 August 2011

Date Author Subject
Fri, 12 Aug 2011 00:00:00 GMT Sukdong Lee Transformation of TOPO Silicatein Back-up

Date: Saturday, 13 August 2011

Date Author Subject
Sat, 13 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of Back-Up Silicatein TOPO

Date: Sunday, 14 August 2011

Date Author Subject
Sun, 14 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of Silicatein TOPO

Date: Monday, 15 August 2011

Date Author Subject
Mon, 15 Aug 2011 00:00:00 GMT Sukdong Lee Digest of pucBB and Silicatein TOPO

Date: Tuesday, 16 August 2011

Date Author Subject
Tue, 16 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB and Gel Extraction
Tue, 16 Aug 2011 18:14:41 GMT Viki Ding Digestion

Date: Wednesday, 17 August 2011

Date Author Subject
Wed, 17 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of Transformants and Gogreen

Date: Thursday, 18 August 2011

Date Author Subject
Thu, 18 Aug 2011 00:00:00 GMT Sukdong Lee Gogreen PCR of Liquid Cultures

Date: Friday, 19 August 2011

Date Author Subject
Fri, 19 Aug 2011 00:00:00 GMT Sukdong Lee Gogreen PCR of Liquid Cultures

Date: Monday, 22 August 2011

Date Author Subject
Mon, 22 Aug 2011 00:00:00 GMT Sukdong Lee Ligation and Transformation
Mon, 22 Aug 2011 10:15:36 GMT Sukdong Lee Minnesota/leex4462-08222011101536-34

Date: Tuesday, 23 August 2011

Date Author Subject
Tue, 23 Aug 2011 00:00:00 GMT Sukdong Lee Ligation of pBAD+Silicatein and Digest of TOPO Silicatein
Tue, 23 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Gogreen PCR of Silicatein+pBAD

Date: Wednesday, 24 August 2011

Date Author Subject
Wed, 24 Aug 2011 00:00:00 GMT Sukdong Lee pBAD+Silicatein transformation and gel extraction of TOPO Silicatein
Wed, 24 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Digest, Transformation of pBAD

Date: Thursday, 25 August 2011

Date Author Subject
Thu, 25 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Gogreen PCR of Silicatein+pBAD
Thu, 25 Aug 2011 00:00:00 GMT Sukdong Lee Minnesota/leex4462-08252011000000-124
Thu, 25 Aug 2011 18:25:40 GMT Viki Ding Cloning #3

Date: Friday, 26 August 2011

Date Author Subject
Fri, 26 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep and Gogreen Check
Fri, 26 Aug 2011 00:00:00 GMT Sukdong Lee Minnesota/leex4462-08262011000000-125

Date: Saturday, 27 August 2011

Date Author Subject
Sat, 27 Aug 2011 18:17:00 GMT Viki Ding Cloning

Date: Monday, 29 August 2011

Date Author Subject
Mon, 29 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Digest, Transformation of pBAD
Mon, 29 Aug 2011 18:30:52 GMT Viki Ding Verifying clones

Date: Tuesday, 30 August 2011

Date Author Subject
Tue, 30 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation
Tue, 30 Aug 2011 18:38:12 GMT Viki Ding SiL & GFP PCT

Date: Wednesday, 31 August 2011

Date Author Subject
Wed, 31 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of pBAD

Date: Friday, 02 September 2011

Date Author Subject
Fri, 02 Sep 2011 00:00:00 GMT Sukdong Lee PCR Overlap Extension (OmpA+Silicatein Fusion)
Fri, 02 Sep 2011 00:01:00 GMT Sukdong Lee Gel of PCR Overlap
Fri, 02 Sep 2011 00:02:00 GMT Sukdong Lee PCR Overlap Test

Date: Tuesday, 06 September 2011

Date Author Subject
Tue, 06 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Overlap
Tue, 06 Sep 2011 00:01:00 GMT Sukdong Lee PCR Overlap Extension
Tue, 06 Sep 2011 18:47:17 GMT Viki Ding Further cloning

Date: Wednesday, 07 September 2011

Date Author Subject
Wed, 07 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Products/Gel Extraction

Date: Thursday, 08 September 2011

Date Author Subject
Thu, 08 Sep 2011 00:00:00 GMT Sukdong Lee PCR Overlap Extension
Thu, 08 Sep 2011 00:01:00 GMT Sukdong Lee Gel Extraction of the PCR Overlap Extension
Thu, 08 Sep 2011 00:03:00 GMT Sukdong Lee Transformation of pBAD+ OmpA
Thu, 08 Sep 2011 00:04:00 GMT Sukdong Lee PCR of Silicatein
Thu, 08 Sep 2011 18:51:05 GMT Viki Ding More cloning

Date: Friday, 09 September 2011

Date Author Subject
Fri, 09 Sep 2011 00:00:00 GMT Sukdong Lee Part II of PCR Overlap
Fri, 09 Sep 2011 00:01:00 GMT Sukdong Lee Liquid Cultures of pBAD and pBAD+OmpA
Fri, 09 Sep 2011 00:02:00 GMT Sukdong Lee PCR of Silicatein
Fri, 09 Sep 2011 00:03:00 GMT Sukdong Lee Gel of Part2+3 and Test of Part 3

Date: Saturday, 10 September 2011

Date Author Subject
Sat, 10 Sep 2011 00:00:00 GMT Sukdong Lee Miniprep of OmpA+pBAD and pBAD
Sat, 10 Sep 2011 00:01:00 GMT Sukdong Lee Gel Extraction of Part 3
Sat, 10 Sep 2011 00:03:00 GMT Sukdong Lee Gel of PCR Silicatein
Sat, 10 Sep 2011 00:04:00 GMT Sukdong Lee PCR Overlap Check

Date: Monday, 12 September 2011

Date Author Subject
Mon, 12 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Products
Mon, 12 Sep 2011 00:01:00 GMT Sukdong Lee PCR of Part 3 and Silicatein
Mon, 12 Sep 2011 18:54:16 GMT Viki Ding Verifying clones

Date: Tuesday, 13 September 2011

Date Author Subject
Tue, 13 Sep 2011 00:00:00 GMT Sukdong Lee PCR of Part 3

Date: Wednesday, 14 September 2011

Date Author Subject
Wed, 14 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR

Date: Friday, 16 September 2011

Date Author Subject
Fri, 16 Sep 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein, part 1, part S, part 2, and part 3
Fri, 16 Sep 2011 00:01:00 GMT Sukdong Lee Gel of PCR Products+Extraction

240px Cbs wdmk blk d2d.jpg
BTIheader white.jpg