Team:Minnesota/leex4462-06222011000000-64

From 2011.igem.org

Mnlogo.jpg
Home Team Project Software Protocols Notebook Attributions Safety

GoGreen PCR and Miniprep of TOPO Liquid Cultures

Project: Surface Display

Date: Wed, 22 Jun 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Tue, 23 Aug 2011 12:11:10 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

-Test liquid cultures for plasmid

-Add 1 uL to GoGreen with OmpA primers and run PCR for 20 cycles. Then check on a gel.

-Miniprep liquid cultures containing the TOPO OmpA.


Procedures:

GoGreen Reaction

-5 uL 2x Gogreen Mix

-0.3 uL Primer 1

-0.3 uL Primer 2

-Template

-For colony pcr=add colony to tube

-For culture pcr=add 0.6 uL

-For minprep pcr=add 0.3 uL

-ddH20 up to 10 uL


Run for 20 cycles.





240px Cbs wdmk blk d2d.jpg
BTIheader white.jpg