Team:Minnesota/leex4462-04142011000000-23

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Gel Electrophoresis of OmpA PCR Product

Project: Surface Display

Date: Thu, 14 Apr 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Mon, 22 Aug 2011 09:18:50 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

To check to see if the ompA gene was replicated. The gene is 1041 base pairs long.


Procedures:

-Mix 0.30 grams of agarose and 30 mL of 1X TAE Buffer.

-Microwave mixture until boiling

-Pour gel and add a drop of Ethidium Bromide. Mix.

-Let the gel and solidify

-Add ladder and DNA.



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