Team:Minnesota/leex4462-08042011000000-95

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Gel Extraction, Ligation, and Transformation

Project: Surface Display

Date: Thu, 04 Aug 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Mon, 29 Aug 2011 13:15:06 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Extract digested silicatein, ligate and transform.


Procedures:

-Left ligation for 3 hours at room temperature.

-Transformed into JM109 and plated on LB+Amp


Results:

-Digested Silicatein=31.9 ng/uL

~10 colonies appeared




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