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Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8

Project: Regulatory

Date: Wed, 10 Aug 2011 14:59:42 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Wed, 10 Aug 2011 15:04:45 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Transformed electrocompetent cells with ligation products of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8. Added 5 ul of each ligation product to 100 ul compentent cells. ho1 ligation product contained excessive salt and caused arc during electroporation. Attempted a second electroporation with 2.5 ul ho1 ligation product which was successful. Plates placed in 37 degree C incubator in Claudia's lab. David Babson will remove plates first thing in the morning and will place them in cooler. NOTE per David: do NOT pick colonies which turn red on the plate.

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