Team:Minnesota/leex4462-06182011000000-61

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PCR of OmpA

Project: Surface Display

Date: Sat, 18 Jun 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Tue, 23 Aug 2011 12:09:54 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

We started another PCR reaction using Taq polymerase to use TOPO kit. The idea is to place the gene into a vector using the TOPO kit to increase the chances for digestions since the enzymes will be able to grab onto the DNA.


Procedures:

-Same as before except the use of Taq instead of Vent.


Results:

In 40 uL of H20,

PCR OmpA=53.3 ng/uL




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