Team:Minnesota/davis950-08032011164451-14

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Digest of ho1 and Cph8

Project: Regulatory

Date: Wed, 03 Aug 2011 16:44:51 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Wed, 03 Aug 2011 16:47:49 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Double digest of ho1 and Cph8 set up and placed in 37 degree C incubator. Reaction mixture was as follows:


  • 3 ul NEBuffer 3 (100% activity for both BglII and NotI)
  • 1 ul BglII
  • 1 ul NotI (acquired from Claudia's lab)
  • 10 ul template DNA
  • 16 ul ddH2O


Digestion should be run for 24 hours and quenched by heating at 65 degree C for 20 minutes.



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