Team:Minnesota/leex4462-06302011000000-75

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Transformation

Project: Surface Display

Date: Thu, 30 Jun 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Thu, 25 Aug 2011 14:45:49 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Transformation of ligation mixture. Used TOP10 chemical mixture and electrocompetent cells.


Procedures:

-Standard heat shock transformation for the TOP10 cells

-Standard electrocompetent transformation but one transformation was n-butanol purified while the other was straight from the ligation mixture.


Notes:

The n-butanol purification is to remove the ligation salts to prevent arcing during the electric shock.



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