From 2011.igem.org
PCR set up
Project: Surface Display
Date: Tue, 26 Apr 2011 15:52:22 GMT
Author: Viki Ding (dingx055)
Access: Public
Revision History:
- Mon, 26 Sep 2011 16:00:18 GMT (dingx055): entry created in project'Surface Display' by dingx055 (id=9)
- Mon, 26 Sep 2011 16:02:39 GMT (dingx055): entry data updated by dingx055 (id=9)
PCR set up for INPNC & GFP.
50 uL Reaction
1 µl vent polymerase
2 µl dNTP
2 µl DNA template
5 µl vent buffer
5 µl forward primer
5 µl reverse primer
30 µl nuclease free water
PCR conditions
95 degrees >> 10 min
95 degrees >> 30 sec
60 degrees >> 1 min
72 degrees >> 1 min
72 degrees >> 10 min
35 cycles
Results: multitple bands, not the correct size
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