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PCR purification of OmpA and Gel Extraction

Project: Surface Display

Date: Wed, 04 May 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

• Mon, 22 Aug 2011 10:46:03 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)
• Tue, 27 Sep 2011 17:07:48 GMT (admin): entry data updated by admin (id=1)

Purpose:

To ensure the Digestion worked and no DNA degradation occurred.

Procedure:

-PCR purify ompA (30uL)

-Run an ompA insert (digested), ompA insert (undigesed, pBAD-plasmid (digested), pBad (undigested)

Lanes

1=Ladder

2-4=pBad digest (40 uL rxn)

5= pBad miniprep

6=ompA digest (5 uL)

7-12= PCR of OmpA PCR Amplification (200ul rxn)

Results:

All suspended in 30 uL:

-pBAD plasmid= 9.7 ng/ul

-OmpA PCR product= 9.0

-OmpA digested=20.8

Revision: Updated 9/27/2011 to include images.

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