Team:Minnesota/leex4462-04212011000000-33

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Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction

Project: Surface Display

Date: Thu, 21 Apr 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

  • Mon, 22 Aug 2011 10:15:27 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Extract the pBAD plasmid from JM109, digest the of insert and vector and purify the cut DNA.


Procedures:

-Same steps as before.

-Another liquid culture of the pBAD was inoculated due to the low yields.


Results:

Tube 2 (insert)= 21.9 ng/ul

Plasmid DNA(undigested miniprep)= 12.9




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