"

From 2011.igem.org

Home	Team	Project	Software	Protocols	Notebook	Attributions	Safety

Restriction Digest of pBAD and OmpA insert

Project: Surface Display

Date: Tue, 03 May 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

• Mon, 22 Aug 2011 10:34:45 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Digest DNA

Procedures:

New procedures

-2 ug of DNA (up to 30 uL)

-2 uL of each enzyme

-4 uL of 10x Buffer

-0.4 uL BSA

-Add H20 up to 40 uL

For OmpA:

104.7 ng/ul x 1 ug/1000 ng= 0.1047 ug/uL x X = 2 ug. => x=19.1 uL (for 40 uL rxn) and 9.55 uL for (20 uL rxn).

For pBAD:

237.7 ng/ul x 1 ug/1000 ng= 0.2377 ug/uL x X = 2 ug. => x=8.41 uL (for 40 uL rxn) and 4.21 uL for (20 uL rxn).

Used 9.6 uL of OmpA and 8.4 uL of pBad.

---

240px