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PCR of Silicatein, Gel Extraction, and TOPO Cloning

Project: Surface Display

Date: Wed, 20 Jul 2011 00:00:00 GMT

Author: Sukdong Lee (leex4462)

Access: Public

Revision History:

• Mon, 29 Aug 2011 13:09:57 GMT (leex4462): entry created in project'Surface Display' by leex4462 (id=3)

Purpose:

Replicate Silicatein gene and place into the TOPO vector

Procedures:

-Same

-Used TOPO kit instruction

-Plated transformants on LB+IPTG, Kan, and XGal.

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