Team:Minnesota/davis950-09102011130654-122

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Digest of pUCBB-lacZ, pBBR, and ompR

Project: Regulatory

Date: Sat, 10 Sep 2011 13:06:54 GMT

Author: Nathan Davis (davis950)

Access: Public

Revision History:

  • Sat, 10 Sep 2011 13:11:38 GMT (davis950): entry created in project'Regulatory' by davis950 (id=2)

Purpose: to digest pUCBB-lacZ, pBBR, and ompR with suitable restriction enzymes to facilitate replacement of pBBR promoter with ompR promoter. Because pBBR is a low copy number plasmid, we have elected to perform the same steps in tandem with pUCBB-lacZ which is a high copy number plasmid.


Procedure:


The following digestion reactions were set up:


  • 16 ul ddH2O
  • 3 ul NE buffer 2
  • 1 ul XbaI
  • 1 ul BglII
  • 10 ul ompR


  • 16 ul ddH2O
  • 3 ul NE buffer 2
  • 1 ul XbaI
  • 1 ul BglII
  • 10 ul pUCBB-lacZ


  • 16 ul ddH2O
  • 3 ul NE buffer 2
  • 1 ul XbaI
  • 1 ul BglII
  • 10 ul pBBR


Results: None



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