Team:Minnesota/Notebook

From 2011.igem.org

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<!-- *** What falls between these lines is the Alert Box!  You can remove it from your pages once you have read and understood the alert *** -->
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__NOTOC__
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{{Team:Minnesota/Top}}
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<br>
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=iGEM 2011 Laboratory Notebook=
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<html>
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==Project: Regulatory==
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<div id="box" style="width: 700px; margin-left: 137px; padding: 5px; border: 3px solid #000; background-color: #fe2b33;">
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===Date: Tuesday, 29 March 2011===
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<div id="template" style="text-align: center; font-weight: bold; font-size: large; color: #f6f6f6; padding: 5px;">
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{| class="wikitable"
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This is a template page. READ THESE INSTRUCTIONS.
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</div>
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<div id="instructions" style="text-align: center; font-weight: normal; font-size: small; color: #f6f6f6; padding: 5px;">
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You are provided with this team page template with which to start the iGEM season.  You may choose to personalize it to fit your team but keep the same "look." Or you may choose to take your team wiki to a different level and design your own wiki.  You can find some examples <a href="https://2008.igem.org/Help:Template/Examples">HERE</a>.
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</div>
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<div id="warning" style="text-align: center; font-weight: bold; font-size: small; color: #f6f6f6; padding: 5px;">
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You <strong>MUST</strong> have a team description page, a project abstract, a complete project description, a lab notebook, and a safety page.  PLEASE keep all of your pages within your teams namespace. 
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</div>
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</div>
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</html>
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<!-- *** End of the alert box *** -->
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{|align="justify"
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|You can write a background of your team here.  Give us a background of your team, the members, etc.  Or tell us more about something of your choosing.
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|[[Image:Minnesota_logo.png|200px|right|frame]]
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! Date
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''Tell us more about your project.  Give us background.  Use this is the abstract of your project.  Be descriptive but concise (1-2 paragraphs)''
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! Author
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|[[Image:Minnesota_team.png|right|frame|Your team picture]]
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! Subject
|-
|-
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|
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| Tue, 29 Mar 2011 12:00:00 GMT
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|align="center"|[[Team:Minnesota | Team Example]]
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| Nathan Davis
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| [[Team:Minnesota/davis950-03292011120000-1|Transformation of comp. cells with registry parts]]
|}
|}
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===Date: Thursday, 07 April 2011===
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<!--- The Mission, Experiments --->
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{| class="wikitable"
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|-
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{| style="color:#1b2c8a;background-color:#0c6;" cellpadding="3" cellspacing="1" border="1" bordercolor="#fff" width="62%" align="center"
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! Date
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!align="center"|[[Team:Minnesota|Home]]
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! Author
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!align="center"|[[Team:Minnesota/Team|Team]]
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! Subject
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!align="center"|[https://igem.org/Team.cgi?year=2010&team_name=Minnesota Official Team Profile]
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|-
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!align="center"|[[Team:Minnesota/Project|Project]]
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| Thu, 07 Apr 2011 15:14:00 GMT
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!align="center"|[[Team:Minnesota/Parts|Parts Submitted to the Registry]]
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| Nathan Davis
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!align="center"|[[Team:Minnesota/Modeling|Modeling]]
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| [[Team:Minnesota/davis950-04072011151400-3|EnvZ mutant comp. cells made]]
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!align="center"|[[Team:Minnesota/Notebook|Notebook]]
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|}
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!align="center"|[[Team:Minnesota/Safety|Safety]]
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===Date: Monday, 18 April 2011===
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!align="center"|[[Team:Minnesota/Attributions|Attributions]]
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Mon, 18 Apr 2011 12:00:00 GMT
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| Nathan Davis
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| [[Team:Minnesota/davis950-04182011120000-4|Registry parts and results of X-gal assay of I15008]]
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|}
 +
===Date: Thursday, 21 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 21 Apr 2011 12:00:00 GMT
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| Nathan Davis
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| [[Team:Minnesota/davis950-04212011120000-5|Transformation of pCph8 from Voigt lab]]
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|}
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===Date: Thursday, 26 May 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 26 May 2011 12:00:00 GMT
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| Nathan Davis
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| [[Team:Minnesota/davis950-05262011120000-7|PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep]]
 +
|}
 +
===Date: Monday, 18 July 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Mon, 18 Jul 2011 14:59:48 GMT
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| Nathan Davis
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| [[Team:Minnesota/davis950-07182011145948-2|Transformation of comp. cell with registry parts]]
 +
|-
 +
| Mon, 18 Jul 2011 15:28:28 GMT
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| Nathan Davis
 +
| [[Team:Minnesota/davis950-07182011152828-8|Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet]]
 +
|}
 +
===Date: Wednesday, 20 July 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Wed, 20 Jul 2011 16:14:10 GMT
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| Nathan Davis
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| [[Team:Minnesota/davis950-07202011161410-9|Miniprep of pUCbb-pBad and pUCbb-pTet]]
 +
|}
 +
===Date: Friday, 22 July 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Fri, 22 Jul 2011 19:58:49 GMT
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| Sung Bong Lee
 +
| [[Team:Minnesota/leex3772-07222011195849-10|run gel with pcr product of ho1 and cph 8 ]]
 +
|}
 +
===Date: Monday, 25 July 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Mon, 25 Jul 2011 14:23:53 GMT
 +
| Sung Bong Lee
 +
| [[Team:Minnesota/leex3772-07252011142353-11|Gel extraction of PCR product of ho1 and Cph8]]
 +
|}
 +
===Date: Thursday, 28 July 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
 +
|-
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| Thu, 28 Jul 2011 20:25:02 GMT
 +
| Sung Bong Lee
 +
| [[Team:Minnesota/leex3772-07282011202502-12|Run gel with NEW PCR product of ho1 and cph8]]
 +
|}
 +
===Date: Wednesday, 03 August 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Wed, 03 Aug 2011 16:41:47 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08032011164147-13|Gel extraction of Cph8 and ho1]]
 +
|-
 +
| Wed, 03 Aug 2011 16:44:51 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08032011164451-14|Digest of ho1 and Cph8]]
 +
|}
 +
===Date: Wednesday, 10 August 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
 +
|-
 +
| Wed, 10 Aug 2011 14:59:42 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08102011145942-15|Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8]]
 +
|}
 +
===Date: Saturday, 13 August 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
 +
|-
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| Sat, 13 Aug 2011 12:35:20 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08132011123520-16|Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1]]
 +
|}
 +
===Date: Tuesday, 16 August 2011===
 +
{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 16 Aug 2011 13:55:44 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08162011135544-17|Digest of pcyA and ho1]]
 +
|}
 +
===Date: Tuesday, 23 August 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 23 Aug 2011 15:50:51 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-08232011155051-72|Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1]]
 +
|}
 +
===Date: Tuesday, 30 August 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 30 Aug 2011 13:22:13 GMT
 +
| Nathan Davis
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| [[Team:Minnesota/davis950-08302011132213-114|Gel extraction and ligation of pcyA and ho1]]
 +
|-
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| Tue, 30 Aug 2011 13:29:13 GMT
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| Nathan Davis
 +
| [[Team:Minnesota/davis950-08302011132913-115|PCR of ompR and silicatein]]
 +
|}
 +
===Date: Thursday, 01 September 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
 +
|-
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| Thu, 01 Sep 2011 14:14:48 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09012011141448-116|Gel extraction of ompR and silicatein]]
 +
|-
 +
| Thu, 01 Sep 2011 14:18:25 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09012011141825-117|Transformation of pcyA/ho1 ligation product]]
 +
|}
 +
===Date: Friday, 09 September 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
 +
|-
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| Fri, 09 Sep 2011 11:45:17 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09092011114517-118|Miniprep of pUC-pcyA-ho1]]
 +
|-
 +
| Fri, 09 Sep 2011 12:13:01 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09092011121301-119|Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8]]
 +
|}
 +
===Date: Saturday, 10 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
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! Subject
 +
|-
 +
| Sat, 10 Sep 2011 13:02:58 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09102011130258-120|Transformation of pUC-pcyA-ho1-Cph8]]
 +
|-
 +
| Sat, 10 Sep 2011 13:05:03 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09102011130503-121|Miniprep of pUCBB-lacZ and pBBR]]
 +
|-
 +
| Sat, 10 Sep 2011 13:06:54 GMT
 +
| Nathan Davis
 +
| [[Team:Minnesota/davis950-09102011130654-122|Digest of pUCBB-lacZ, pBBR, and ompR]]
 +
|}
 +
==Project: Surface Display==
 +
===Date: Thursday, 07 April 2011===
 +
{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 07 Apr 2011 11:20:00 GMT
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| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04072011112000-18|Primer Design for OmpA]]
 +
|}
 +
===Date: Sunday, 10 April 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
 +
|-
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| Sun, 10 Apr 2011 00:00:00 GMT
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| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04102011000000-19|OmpA BL21 Genomic Liquid Cultures]]
 +
|}
 +
===Date: Monday, 11 April 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
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|-
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| Mon, 11 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04112011000000-20|OmpA BL21 Genomic Extraction]]
 +
|}
 +
===Date: Thursday, 14 April 2011===
 +
{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-21|PCR Amplification of OmpA]]
 +
|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-22|pucBB Liquid Cultures]]
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|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-23|Gel Electrophoresis of OmpA PCR Product]]
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|-
 +
| Thu, 14 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04142011000000-24|Gel Purification of OmpA PCR Products]]
 +
|}
 +
===Date: Friday, 15 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Fri, 15 Apr 2011 00:00:00 GMT
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| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04152011000000-25|Miniprep of pucBB+GFP]]
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|-
 +
| Fri, 15 Apr 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04152011000000-26|Restriction Digest of pucBB+GFP and Gel extraction of OmpA]]
 +
|}
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===Date: Saturday, 16 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Sat, 16 Apr 2011 00:01:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04162011000100-27|Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP]]
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|-
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| Sat, 16 Apr 2011 00:02:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04162011000200-28|Restriction Digest of pucBB and ompA inserts]]
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|-
 +
| Sat, 16 Apr 2011 00:03:00 GMT
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| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-04162011000300-29|Gel Electrophoresis of OmpA ]]
 +
|}
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===Date: Tuesday, 19 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 19 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04192011000000-30|PCR Amplification of Genomic BL21]]
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|-
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| Tue, 19 Apr 2011 00:01:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04192011000100-31|Gel Extraction of OmpA]]
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|}
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===Date: Wednesday, 20 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Wed, 20 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04202011000000-32|pBAD plasmid liquid cultures]]
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|}
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===Date: Thursday, 21 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 21 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04212011000000-33|Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction]]
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|-
 +
| Thu, 21 Apr 2011 12:00:00 GMT
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| Nathan Davis
 +
| [[Team:Minnesota/davis950-04212011120000-6|Gel extraction of Silicatein alpha DNA]]
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|}
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===Date: Friday, 22 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Fri, 22 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04222011000000-40|Miniprep, Digest, and Extract]]
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|}
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===Date: Saturday, 23 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Sat, 23 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04232011000000-35|Ligation of pBAD and OmpA]]
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|}
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===Date: Tuesday, 26 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 26 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04262011000000-36|Restriction digest of OmpA, PCR of OmpA (back-up)]]
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|-
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| Tue, 26 Apr 2011 15:52:22 GMT
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| Viki Ding
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| [[Team:Minnesota/dingx055-04262011155222-153|PCR set up]]
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|-
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| Tue, 26 Apr 2011 16:02:53 GMT
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| Viki Ding
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| [[Team:Minnesota/dingx055-04262011160253-154|PCR  trial #2]]
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|}
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===Date: Thursday, 28 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 28 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04282011000000-37|PCR of OmpA and Gel]]
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|}
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===Date: Saturday, 30 April 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Sat, 30 Apr 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-04302011000000-38|Speed Vacuum]]
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|}
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===Date: Tuesday, 03 May 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 03 May 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-05032011000000-39|Restriction Digest of pBAD and OmpA insert]]
 +
|}
 +
===Date: Wednesday, 04 May 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Wed, 04 May 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-05042011000000-41|PCR purification of OmpA and Gel Extraction]]
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|}
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===Date: Thursday, 05 May 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Thu, 05 May 2011 00:00:00 GMT
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| Sukdong Lee
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| [[Team:Minnesota/leex4462-05052011000000-42|Ligation of OmpA and pBAD]]
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|}
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===Date: Friday, 06 May 2011===
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{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Fri, 06 May 2011 00:00:00 GMT
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| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05062011000000-43|Transformation]]
 +
|}
 +
===Date: Monday, 23 May 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
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|-
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| Mon, 23 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05232011000000-44|Transformation and Liquid cultures of OmpA transformants]]
 +
|}
 +
===Date: Tuesday, 24 May 2011===
 +
{| class="wikitable"
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|-
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! Date
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! Author
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! Subject
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|-
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| Tue, 24 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05242011000000-45|Miniprep and PCR, Liquid Cultures ]]
 +
|}
 +
===Date: Wednesday, 25 May 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
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|-
 +
| Wed, 25 May 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-05252011000000-46|Gel of OmpA Plasmid]]
 +
|}
 +
===Date: Wednesday, 01 June 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
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|-
 +
| Wed, 01 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06012011000000-47|Liquid cultures of pBAD plasmid]]
 +
|}
 +
===Date: Thursday, 02 June 2011===
 +
{| class="wikitable"
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|-
 +
! Date
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! Author
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! Subject
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|-
 +
| Thu, 02 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06022011000000-48|PCR Amplification of OmpA and Miniprep of pBADBB]]
 +
|}
 +
===Date: Friday, 03 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 03 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06032011000000-49|Gel Electrophoresis of OmpA and pBAD PCR Products and Digests]]
 +
|-
 +
| Fri, 03 Jun 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06032011000100-50|Extractions of OmpA]]
 +
|}
 +
===Date: Sunday, 05 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 05 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06052011000000-51|Speed Vac and Digests]]
 +
|}
 +
===Date: Monday, 06 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 06 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06062011000000-52|Gel and Extraction]]
 +
|}
 +
===Date: Tuesday, 07 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 07 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06072011000000-53|Digestion of OmpA]]
 +
|}
 +
===Date: Wednesday, 08 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 08 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06082011000000-54|PCR Purification/Gel Extraction]]
 +
|}
 +
===Date: Thursday, 09 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 09 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06092011000000-55|Ligation of OmpA and pBAD]]
 +
|}
 +
===Date: Friday, 10 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 10 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06102011000000-56|Transformation of OmpA plasmid ]]
 +
|}
 +
===Date: Sunday, 12 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 12 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06122011000000-57|Electroporation transformation]]
 +
|}
 +
===Date: Monday, 13 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 13 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06132011000000-58|Ligation of OmpA+pBAD]]
 +
|}
 +
===Date: Tuesday, 14 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 14 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06142011000000-59|Ligation]]
 +
|}
 +
===Date: Wednesday, 15 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 15 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06152011000000-60|Electroporation Transformation]]
 +
|}
 +
===Date: Saturday, 18 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 18 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06182011000000-61|PCR of OmpA]]
 +
|}
 +
===Date: Monday, 20 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 20 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06202011000000-62|TOPO Cloning and Gel Extraction]]
 +
|}
 +
===Date: Tuesday, 21 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 21 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06212011000000-63|Liquid Cultures of TOPO Cloning]]
 +
|}
 +
===Date: Wednesday, 22 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 22 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06222011000000-64|GoGreen PCR and Miniprep of TOPO Liquid Cultures]]
 +
|}
 +
===Date: Thursday, 23 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 23 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06232011000000-65|Restriction Digests]]
 +
|}
 +
===Date: Friday, 24 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 24 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06242011000000-66|Gel Extraction and Ligation]]
 +
|}
 +
===Date: Saturday, 25 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 25 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06252011000000-67|Electroporation]]
 +
|-
 +
| Sat, 25 Jun 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06252011000100-68|Chemical Transformation]]
 +
|}
 +
===Date: Sunday, 26 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 26 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06262011000000-69|Liquid cultures of OmpA plasmids]]
 +
|}
 +
===Date: Monday, 27 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 27 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06272011000000-70|Miniprep of OmpA and pBAD plasmid]]
 +
|}
 +
===Date: Tuesday, 28 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 28 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06282011000000-71|Gogreen PCR and Digestion Check]]
 +
|-
 +
| Tue, 28 Jun 2011 16:10:19 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-06282011161019-155|PCR of INP]]
 +
|}
 +
===Date: Wednesday, 29 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 29 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06292011000000-73|Silicatein: Digest of pucBB]]
 +
|-
 +
| Wed, 29 Jun 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06292011000100-74|Ligation of pucBB plasmid and Silicatein]]
 +
|}
 +
===Date: Thursday, 30 June 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 30 Jun 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-06302011000000-75|Transformation]]
 +
|}
 +
===Date: Friday, 01 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 01 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07012011000000-76|Liquid cultures of pucBB+Silicatein Transformants]]
 +
|}
 +
===Date: Saturday, 02 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 02 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07022011000000-77|Miniprep of Silicatein+pucBB transformants]]
 +
|}
 +
===Date: Monday, 04 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 04 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07042011000000-78|Liquid Gogreen PCR ]]
 +
|}
 +
===Date: Wednesday, 06 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 06 Jul 2011 16:15:53 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07062011161553-156|INP PCR clean up]]
 +
|}
 +
===Date: Thursday, 07 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 07 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07072011000000-79|Gogreen of Silicatein pucBB plasmid]]
 +
|-
 +
| Thu, 07 Jul 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07072011000100-80|Restriction Digest of pucBB and PCR of Silicatein]]
 +
|}
 +
===Date: Friday, 08 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 08 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07082011000000-81|Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation]]
 +
|}
 +
===Date: Monday, 11 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 11 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07112011000000-82|Gel Extraction of Silicatein and Liquid Cultures of pucBB]]
 +
|}
 +
===Date: Tuesday, 12 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 12 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07122011000000-83|Transformation of pucBB+Silicatein]]
 +
|-
 +
| Tue, 12 Jul 2011 16:17:17 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07122011161717-157|Digestion of INP]]
 +
|}
 +
===Date: Wednesday, 13 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 13 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07132011000000-84|Gogreen PCR and Miniprep]]
 +
|-
 +
| Wed, 13 Jul 2011 16:20:04 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07132011162004-158|INP cloning]]
 +
|}
 +
===Date: Friday, 15 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 15 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07152011000000-85|pucBB Digest and pucBB Liquid Cultures]]
 +
|}
 +
===Date: Saturday, 16 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 16 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07162011000000-86|Miniprep of pucBB and gel extraction of pucBB]]
 +
|}
 +
===Date: Wednesday, 20 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 20 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07202011000000-87|PCR of Silicatein, Gel Extraction, and TOPO Cloning]]
 +
|-
 +
| Wed, 20 Jul 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07202011000100-88|TOPO Transformants and Liquid Cultures]]
 +
|-
 +
| Wed, 20 Jul 2011 17:59:22 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07202011175922-159|Transformation trial #2]]
 +
|}
 +
===Date: Friday, 22 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 22 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07222011000000-89|TOPO Miniprep and Gogreen PCR and Gel]]
 +
|}
 +
===Date: Monday, 25 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 25 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07252011000000-90|Gogreen PCR X3 and Gel X3]]
 +
|}
 +
===Date: Tuesday, 26 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 26 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07262011000000-91|PCR of Silicatein and TOPO]]
 +
|-
 +
| Tue, 26 Jul 2011 18:02:01 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07262011180201-160|INP PCR with Taq mix]]
 +
|}
 +
===Date: Wednesday, 27 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 27 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07272011000000-92|Liquid Cultures and Colony Gogreen PCR]]
 +
|-
 +
| Wed, 27 Jul 2011 18:06:41 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-07272011180641-161|INP PCR w/Taq & digestion]]
 +
|}
 +
===Date: Thursday, 28 July 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 28 Jul 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-07282011000000-93|Miniprep and Sequencing of the TOPO Silicatein]]
 +
|}
 +
===Date: Tuesday, 02 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 02 Aug 2011 18:12:35 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08022011181235-162|Ligation & transformation ]]
 +
|}
 +
===Date: Wednesday, 03 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 03 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08032011000000-94|Digestion of Silicatein TOPO 7]]
 +
|}
 +
===Date: Thursday, 04 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 04 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08042011000000-95|Gel Extraction, Ligation, and Transformation]]
 +
|}
 +
===Date: Monday, 08 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 08 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08082011000000-96|Liquid Cultures, Master Plate, Gogreen, and Gel]]
 +
|}
 +
===Date: Tuesday, 09 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 09 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08092011000000-97|Miniprep, Gogreen Liquid PCR, and Gel]]
 +
|}
 +
===Date: Wednesday, 10 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 10 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08102011000000-98|Liquid Cultures, Liquid Gogreen PCR]]
 +
|}
 +
===Date: Thursday, 11 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 11 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08112011000000-99|More Liquid Cultures, Liquid Gogreen PCR]]
 +
|}
 +
===Date: Friday, 12 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 12 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08122011000000-100|Transformation of TOPO Silicatein Back-up]]
 +
|}
 +
===Date: Saturday, 13 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 13 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08132011000000-101|Liquid Cultures of Back-Up Silicatein TOPO]]
 +
|}
 +
===Date: Sunday, 14 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sun, 14 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08142011000000-102|Miniprep of Silicatein TOPO]]
 +
|}
 +
===Date: Monday, 15 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 15 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08152011000000-103|Digest of pucBB and Silicatein TOPO]]
 +
|}
 +
===Date: Tuesday, 16 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 16 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08162011000000-104|Miniprep of pucBB and Gel Extraction]]
 +
|-
 +
| Tue, 16 Aug 2011 18:14:41 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08162011181441-163|Digestion]]
 +
|}
 +
===Date: Wednesday, 17 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 17 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08172011000000-105|Liquid Cultures of Transformants and Gogreen]]
 +
|}
 +
===Date: Thursday, 18 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 18 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08182011000000-106|Gogreen PCR of Liquid Cultures]]
 +
|}
 +
===Date: Friday, 19 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 19 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08192011000000-107|Gogreen PCR of Liquid Cultures]]
 +
|}
 +
===Date: Monday, 22 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 22 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08222011000000-108|Ligation and Transformation]]
 +
|-
 +
| Mon, 22 Aug 2011 10:15:36 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08222011101536-34|Minnesota/leex4462-08222011101536-34]]
 +
|}
 +
===Date: Tuesday, 23 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 23 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08232011000000-109|Ligation of pBAD+Silicatein and Digest of TOPO Silicatein]]
 +
|-
 +
| Tue, 23 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08232011000000-110|Liquid Cultures, Gogreen PCR of Silicatein+pBAD]]
 +
|}
 +
===Date: Wednesday, 24 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 24 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08242011000000-111|pBAD+Silicatein transformation and gel extraction of TOPO Silicatein]]
 +
|-
 +
| Wed, 24 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08242011000000-123|Ligation, Digest, Transformation of pBAD]]
 +
|}
 +
===Date: Thursday, 25 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 25 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08252011000000-112|Liquid Cultures, Gogreen PCR of Silicatein+pBAD]]
 +
|-
 +
| Thu, 25 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08252011000000-124|Minnesota/leex4462-08252011000000-124]]
 +
|-
 +
| Thu, 25 Aug 2011 18:25:40 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08252011182540-165| Cloning #3]]
 +
|}
 +
===Date: Friday, 26 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 26 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08262011000000-113|Miniprep and Gogreen Check]]
 +
|-
 +
| Fri, 26 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08262011000000-125|Minnesota/leex4462-08262011000000-125]]
 +
|}
 +
===Date: Saturday, 27 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 27 Aug 2011 18:17:00 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08272011181700-164|Cloning]]
 +
|}
 +
===Date: Monday, 29 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 29 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08292011000000-126|Ligation, Digest, Transformation of pBAD]]
 +
|-
 +
| Mon, 29 Aug 2011 18:30:52 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08292011183052-166|Verifying clones]]
 +
|}
 +
===Date: Tuesday, 30 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 30 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08302011000000-127|Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation]]
 +
|-
 +
| Tue, 30 Aug 2011 18:38:12 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-08302011183812-167|SiL & GFP PCT]]
 +
|}
 +
===Date: Wednesday, 31 August 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 31 Aug 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-08312011000000-128|Miniprep of pBAD]]
 +
|}
 +
===Date: Friday, 02 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 02 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09022011000000-129|PCR Overlap Extension (OmpA+Silicatein Fusion)]]
 +
|-
 +
| Fri, 02 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09022011000100-130|Gel of PCR Overlap]]
 +
|-
 +
| Fri, 02 Sep 2011 00:02:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09022011000200-131|PCR Overlap Test]]
 +
|}
 +
===Date: Tuesday, 06 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 06 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09062011000000-132|Gel of PCR Overlap ]]
 +
|-
 +
| Tue, 06 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09062011000100-133|PCR Overlap Extension]]
 +
|-
 +
| Tue, 06 Sep 2011 18:47:17 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-09062011184717-168|Further cloning]]
 +
|}
 +
===Date: Wednesday, 07 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 07 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09072011000000-134|Gel of PCR Products/Gel Extraction]]
 +
|}
 +
===Date: Thursday, 08 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Thu, 08 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000000-135|PCR Overlap Extension]]
 +
|-
 +
| Thu, 08 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000100-136|Gel Extraction of the PCR Overlap Extension]]
 +
|-
 +
| Thu, 08 Sep 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000300-137|Transformation of pBAD+ OmpA]]
 +
|-
 +
| Thu, 08 Sep 2011 00:04:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09082011000400-138|PCR of Silicatein]]
 +
|-
 +
| Thu, 08 Sep 2011 18:51:05 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-09082011185105-169|More cloning]]
 +
|}
 +
===Date: Friday, 09 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 09 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000000-139|Part II of PCR Overlap]]
 +
|-
 +
| Fri, 09 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000100-140|Liquid Cultures of pBAD and pBAD+OmpA]]
 +
|-
 +
| Fri, 09 Sep 2011 00:02:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000200-141|PCR of Silicatein ]]
 +
|-
 +
| Fri, 09 Sep 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09092011000300-142|Gel of Part2+3 and Test of Part 3]]
 +
|}
 +
===Date: Saturday, 10 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Sat, 10 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000000-143|Miniprep of OmpA+pBAD and pBAD]]
 +
|-
 +
| Sat, 10 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000100-144|Gel Extraction of Part 3]]
 +
|-
 +
| Sat, 10 Sep 2011 00:03:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000300-145|Gel of PCR Silicatein]]
 +
|-
 +
| Sat, 10 Sep 2011 00:04:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09102011000400-146|PCR Overlap Check]]
 +
|}
 +
===Date: Monday, 12 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Mon, 12 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09122011000000-147|Gel of PCR Products]]
 +
|-
 +
| Mon, 12 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09122011000100-148|PCR of Part 3 and Silicatein]]
 +
|-
 +
| Mon, 12 Sep 2011 18:54:16 GMT
 +
| Viki Ding
 +
| [[Team:Minnesota/dingx055-09122011185416-170|Verifying clones]]
 +
|}
 +
===Date: Tuesday, 13 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Tue, 13 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09132011000000-149|PCR of Part 3]]
 +
|}
 +
===Date: Wednesday, 14 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Wed, 14 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09142011000000-150|Gel of PCR]]
 +
|}
 +
===Date: Friday, 16 September 2011===
 +
{| class="wikitable"
 +
|-
 +
! Date
 +
! Author
 +
! Subject
 +
|-
 +
| Fri, 16 Sep 2011 00:00:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09162011000000-151|PCR of Silicatein, part 1, part S, part 2, and part 3]]
 +
|-
 +
| Fri, 16 Sep 2011 00:01:00 GMT
 +
| Sukdong Lee
 +
| [[Team:Minnesota/leex4462-09162011000100-152|Gel of PCR Products+Extraction]]
|}
|}
-
 
+
{{Team:Minnesota/Bottom}}
-
==Notebook==
+
-
 
+
-
You should make use of the calendar feature on the wiki and start a lab notebook.  This may be looked at by the judges to see how your work progressed throughout the summer.  It is a very useful organizational tool as well.
+

Latest revision as of 22:46, 27 September 2011

Mnlogo.jpg
Home Team Project Software Protocols Notebook Attributions Safety

iGEM 2011 Laboratory Notebook

Project: Regulatory

Date: Tuesday, 29 March 2011

Date Author Subject
Tue, 29 Mar 2011 12:00:00 GMT Nathan Davis Transformation of comp. cells with registry parts

Date: Thursday, 07 April 2011

Date Author Subject
Thu, 07 Apr 2011 15:14:00 GMT Nathan Davis EnvZ mutant comp. cells made

Date: Monday, 18 April 2011

Date Author Subject
Mon, 18 Apr 2011 12:00:00 GMT Nathan Davis Registry parts and results of X-gal assay of I15008

Date: Thursday, 21 April 2011

Date Author Subject
Thu, 21 Apr 2011 12:00:00 GMT Nathan Davis Transformation of pCph8 from Voigt lab

Date: Thursday, 26 May 2011

Date Author Subject
Thu, 26 May 2011 12:00:00 GMT Nathan Davis PCR of Syneccocystis sp 6803 genomic DNA and pCph8 miniprep

Date: Monday, 18 July 2011

Date Author Subject
Mon, 18 Jul 2011 14:59:48 GMT Nathan Davis Transformation of comp. cell with registry parts
Mon, 18 Jul 2011 15:28:28 GMT Nathan Davis Transformation with pUC-BB, pUCbb-pBad, and pUCbb-pTet

Date: Wednesday, 20 July 2011

Date Author Subject
Wed, 20 Jul 2011 16:14:10 GMT Nathan Davis Miniprep of pUCbb-pBad and pUCbb-pTet

Date: Friday, 22 July 2011

Date Author Subject
Fri, 22 Jul 2011 19:58:49 GMT Sung Bong Lee run gel with pcr product of ho1 and cph 8

Date: Monday, 25 July 2011

Date Author Subject
Mon, 25 Jul 2011 14:23:53 GMT Sung Bong Lee Gel extraction of PCR product of ho1 and Cph8

Date: Thursday, 28 July 2011

Date Author Subject
Thu, 28 Jul 2011 20:25:02 GMT Sung Bong Lee Run gel with NEW PCR product of ho1 and cph8

Date: Wednesday, 03 August 2011

Date Author Subject
Wed, 03 Aug 2011 16:41:47 GMT Nathan Davis Gel extraction of Cph8 and ho1
Wed, 03 Aug 2011 16:44:51 GMT Nathan Davis Digest of ho1 and Cph8

Date: Wednesday, 10 August 2011

Date Author Subject
Wed, 10 Aug 2011 14:59:42 GMT Nathan Davis Transformation of pucBB-pBad-ho1, pucBB-pBad-pcyA, and pucBB-pTet-Cph8

Date: Saturday, 13 August 2011

Date Author Subject
Sat, 13 Aug 2011 12:35:20 GMT Nathan Davis Miniprep of pucBB-pTet-Cph8, pucBB-pTet-pcyA, and pucBB-pTet-ho1

Date: Tuesday, 16 August 2011

Date Author Subject
Tue, 16 Aug 2011 13:55:44 GMT Nathan Davis Digest of pcyA and ho1

Date: Tuesday, 23 August 2011

Date Author Subject
Tue, 23 Aug 2011 15:50:51 GMT Nathan Davis Digest of pUCBB-pBad-pcyA pUCBB-pBad-ho1

Date: Tuesday, 30 August 2011

Date Author Subject
Tue, 30 Aug 2011 13:22:13 GMT Nathan Davis Gel extraction and ligation of pcyA and ho1
Tue, 30 Aug 2011 13:29:13 GMT Nathan Davis PCR of ompR and silicatein

Date: Thursday, 01 September 2011

Date Author Subject
Thu, 01 Sep 2011 14:14:48 GMT Nathan Davis Gel extraction of ompR and silicatein
Thu, 01 Sep 2011 14:18:25 GMT Nathan Davis Transformation of pcyA/ho1 ligation product

Date: Friday, 09 September 2011

Date Author Subject
Fri, 09 Sep 2011 11:45:17 GMT Nathan Davis Miniprep of pUC-pcyA-ho1
Fri, 09 Sep 2011 12:13:01 GMT Nathan Davis Digest of pUC-pcyA-ho1 and pUC-pTet-Cph8

Date: Saturday, 10 September 2011

Date Author Subject
Sat, 10 Sep 2011 13:02:58 GMT Nathan Davis Transformation of pUC-pcyA-ho1-Cph8
Sat, 10 Sep 2011 13:05:03 GMT Nathan Davis Miniprep of pUCBB-lacZ and pBBR
Sat, 10 Sep 2011 13:06:54 GMT Nathan Davis Digest of pUCBB-lacZ, pBBR, and ompR

Project: Surface Display

Date: Thursday, 07 April 2011

Date Author Subject
Thu, 07 Apr 2011 11:20:00 GMT Sukdong Lee Primer Design for OmpA

Date: Sunday, 10 April 2011

Date Author Subject
Sun, 10 Apr 2011 00:00:00 GMT Sukdong Lee OmpA BL21 Genomic Liquid Cultures

Date: Monday, 11 April 2011

Date Author Subject
Mon, 11 Apr 2011 00:00:00 GMT Sukdong Lee OmpA BL21 Genomic Extraction

Date: Thursday, 14 April 2011

Date Author Subject
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee PCR Amplification of OmpA
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee pucBB Liquid Cultures
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee Gel Electrophoresis of OmpA PCR Product
Thu, 14 Apr 2011 00:00:00 GMT Sukdong Lee Gel Purification of OmpA PCR Products

Date: Friday, 15 April 2011

Date Author Subject
Fri, 15 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB+GFP
Fri, 15 Apr 2011 00:00:00 GMT Sukdong Lee Restriction Digest of pucBB+GFP and Gel extraction of OmpA

Date: Saturday, 16 April 2011

Date Author Subject
Sat, 16 Apr 2011 00:01:00 GMT Sukdong Lee Gel Electrophoresis of Restriction Digest of OmpA and pucBB+GFP
Sat, 16 Apr 2011 00:02:00 GMT Sukdong Lee Restriction Digest of pucBB and ompA inserts
Sat, 16 Apr 2011 00:03:00 GMT Sukdong Lee Gel Electrophoresis of OmpA

Date: Tuesday, 19 April 2011

Date Author Subject
Tue, 19 Apr 2011 00:00:00 GMT Sukdong Lee PCR Amplification of Genomic BL21
Tue, 19 Apr 2011 00:01:00 GMT Sukdong Lee Gel Extraction of OmpA

Date: Wednesday, 20 April 2011

Date Author Subject
Wed, 20 Apr 2011 00:00:00 GMT Sukdong Lee pBAD plasmid liquid cultures

Date: Thursday, 21 April 2011

Date Author Subject
Thu, 21 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep, Restriction Digest of OmpA and pucBB, and Gel extraction
Thu, 21 Apr 2011 12:00:00 GMT Nathan Davis Gel extraction of Silicatein alpha DNA

Date: Friday, 22 April 2011

Date Author Subject
Fri, 22 Apr 2011 00:00:00 GMT Sukdong Lee Miniprep, Digest, and Extract

Date: Saturday, 23 April 2011

Date Author Subject
Sat, 23 Apr 2011 00:00:00 GMT Sukdong Lee Ligation of pBAD and OmpA

Date: Tuesday, 26 April 2011

Date Author Subject
Tue, 26 Apr 2011 00:00:00 GMT Sukdong Lee Restriction digest of OmpA, PCR of OmpA (back-up)
Tue, 26 Apr 2011 15:52:22 GMT Viki Ding PCR set up
Tue, 26 Apr 2011 16:02:53 GMT Viki Ding PCR trial #2

Date: Thursday, 28 April 2011

Date Author Subject
Thu, 28 Apr 2011 00:00:00 GMT Sukdong Lee PCR of OmpA and Gel

Date: Saturday, 30 April 2011

Date Author Subject
Sat, 30 Apr 2011 00:00:00 GMT Sukdong Lee Speed Vacuum

Date: Tuesday, 03 May 2011

Date Author Subject
Tue, 03 May 2011 00:00:00 GMT Sukdong Lee Restriction Digest of pBAD and OmpA insert

Date: Wednesday, 04 May 2011

Date Author Subject
Wed, 04 May 2011 00:00:00 GMT Sukdong Lee PCR purification of OmpA and Gel Extraction

Date: Thursday, 05 May 2011

Date Author Subject
Thu, 05 May 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA and pBAD

Date: Friday, 06 May 2011

Date Author Subject
Fri, 06 May 2011 00:00:00 GMT Sukdong Lee Transformation

Date: Monday, 23 May 2011

Date Author Subject
Mon, 23 May 2011 00:00:00 GMT Sukdong Lee Transformation and Liquid cultures of OmpA transformants

Date: Tuesday, 24 May 2011

Date Author Subject
Tue, 24 May 2011 00:00:00 GMT Sukdong Lee Miniprep and PCR, Liquid Cultures

Date: Wednesday, 25 May 2011

Date Author Subject
Wed, 25 May 2011 00:00:00 GMT Sukdong Lee Gel of OmpA Plasmid

Date: Wednesday, 01 June 2011

Date Author Subject
Wed, 01 Jun 2011 00:00:00 GMT Sukdong Lee Liquid cultures of pBAD plasmid

Date: Thursday, 02 June 2011

Date Author Subject
Thu, 02 Jun 2011 00:00:00 GMT Sukdong Lee PCR Amplification of OmpA and Miniprep of pBADBB

Date: Friday, 03 June 2011

Date Author Subject
Fri, 03 Jun 2011 00:00:00 GMT Sukdong Lee Gel Electrophoresis of OmpA and pBAD PCR Products and Digests
Fri, 03 Jun 2011 00:01:00 GMT Sukdong Lee Extractions of OmpA

Date: Sunday, 05 June 2011

Date Author Subject
Sun, 05 Jun 2011 00:00:00 GMT Sukdong Lee Speed Vac and Digests

Date: Monday, 06 June 2011

Date Author Subject
Mon, 06 Jun 2011 00:00:00 GMT Sukdong Lee Gel and Extraction

Date: Tuesday, 07 June 2011

Date Author Subject
Tue, 07 Jun 2011 00:00:00 GMT Sukdong Lee Digestion of OmpA

Date: Wednesday, 08 June 2011

Date Author Subject
Wed, 08 Jun 2011 00:00:00 GMT Sukdong Lee PCR Purification/Gel Extraction

Date: Thursday, 09 June 2011

Date Author Subject
Thu, 09 Jun 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA and pBAD

Date: Friday, 10 June 2011

Date Author Subject
Fri, 10 Jun 2011 00:00:00 GMT Sukdong Lee Transformation of OmpA plasmid

Date: Sunday, 12 June 2011

Date Author Subject
Sun, 12 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation transformation

Date: Monday, 13 June 2011

Date Author Subject
Mon, 13 Jun 2011 00:00:00 GMT Sukdong Lee Ligation of OmpA+pBAD

Date: Tuesday, 14 June 2011

Date Author Subject
Tue, 14 Jun 2011 00:00:00 GMT Sukdong Lee Ligation

Date: Wednesday, 15 June 2011

Date Author Subject
Wed, 15 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation Transformation

Date: Saturday, 18 June 2011

Date Author Subject
Sat, 18 Jun 2011 00:00:00 GMT Sukdong Lee PCR of OmpA

Date: Monday, 20 June 2011

Date Author Subject
Mon, 20 Jun 2011 00:00:00 GMT Sukdong Lee TOPO Cloning and Gel Extraction

Date: Tuesday, 21 June 2011

Date Author Subject
Tue, 21 Jun 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of TOPO Cloning

Date: Wednesday, 22 June 2011

Date Author Subject
Wed, 22 Jun 2011 00:00:00 GMT Sukdong Lee GoGreen PCR and Miniprep of TOPO Liquid Cultures

Date: Thursday, 23 June 2011

Date Author Subject
Thu, 23 Jun 2011 00:00:00 GMT Sukdong Lee Restriction Digests

Date: Friday, 24 June 2011

Date Author Subject
Fri, 24 Jun 2011 00:00:00 GMT Sukdong Lee Gel Extraction and Ligation

Date: Saturday, 25 June 2011

Date Author Subject
Sat, 25 Jun 2011 00:00:00 GMT Sukdong Lee Electroporation
Sat, 25 Jun 2011 00:01:00 GMT Sukdong Lee Chemical Transformation

Date: Sunday, 26 June 2011

Date Author Subject
Sun, 26 Jun 2011 00:00:00 GMT Sukdong Lee Liquid cultures of OmpA plasmids

Date: Monday, 27 June 2011

Date Author Subject
Mon, 27 Jun 2011 00:00:00 GMT Sukdong Lee Miniprep of OmpA and pBAD plasmid

Date: Tuesday, 28 June 2011

Date Author Subject
Tue, 28 Jun 2011 00:00:00 GMT Sukdong Lee Gogreen PCR and Digestion Check
Tue, 28 Jun 2011 16:10:19 GMT Viki Ding PCR of INP

Date: Wednesday, 29 June 2011

Date Author Subject
Wed, 29 Jun 2011 00:00:00 GMT Sukdong Lee Silicatein: Digest of pucBB
Wed, 29 Jun 2011 00:01:00 GMT Sukdong Lee Ligation of pucBB plasmid and Silicatein

Date: Thursday, 30 June 2011

Date Author Subject
Thu, 30 Jun 2011 00:00:00 GMT Sukdong Lee Transformation

Date: Friday, 01 July 2011

Date Author Subject
Fri, 01 Jul 2011 00:00:00 GMT Sukdong Lee Liquid cultures of pucBB+Silicatein Transformants

Date: Saturday, 02 July 2011

Date Author Subject
Sat, 02 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep of Silicatein+pucBB transformants

Date: Monday, 04 July 2011

Date Author Subject
Mon, 04 Jul 2011 00:00:00 GMT Sukdong Lee Liquid Gogreen PCR

Date: Wednesday, 06 July 2011

Date Author Subject
Wed, 06 Jul 2011 16:15:53 GMT Viki Ding INP PCR clean up

Date: Thursday, 07 July 2011

Date Author Subject
Thu, 07 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen of Silicatein pucBB plasmid
Thu, 07 Jul 2011 00:01:00 GMT Sukdong Lee Restriction Digest of pucBB and PCR of Silicatein

Date: Friday, 08 July 2011

Date Author Subject
Fri, 08 Jul 2011 00:00:00 GMT Sukdong Lee Gel extraction of Silicatein, Liquid Culture of pucBB, and Ligation

Date: Monday, 11 July 2011

Date Author Subject
Mon, 11 Jul 2011 00:00:00 GMT Sukdong Lee Gel Extraction of Silicatein and Liquid Cultures of pucBB

Date: Tuesday, 12 July 2011

Date Author Subject
Tue, 12 Jul 2011 00:00:00 GMT Sukdong Lee Transformation of pucBB+Silicatein
Tue, 12 Jul 2011 16:17:17 GMT Viki Ding Digestion of INP

Date: Wednesday, 13 July 2011

Date Author Subject
Wed, 13 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen PCR and Miniprep
Wed, 13 Jul 2011 16:20:04 GMT Viki Ding INP cloning

Date: Friday, 15 July 2011

Date Author Subject
Fri, 15 Jul 2011 00:00:00 GMT Sukdong Lee pucBB Digest and pucBB Liquid Cultures

Date: Saturday, 16 July 2011

Date Author Subject
Sat, 16 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB and gel extraction of pucBB

Date: Wednesday, 20 July 2011

Date Author Subject
Wed, 20 Jul 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein, Gel Extraction, and TOPO Cloning
Wed, 20 Jul 2011 00:01:00 GMT Sukdong Lee TOPO Transformants and Liquid Cultures
Wed, 20 Jul 2011 17:59:22 GMT Viki Ding Transformation trial #2

Date: Friday, 22 July 2011

Date Author Subject
Fri, 22 Jul 2011 00:00:00 GMT Sukdong Lee TOPO Miniprep and Gogreen PCR and Gel

Date: Monday, 25 July 2011

Date Author Subject
Mon, 25 Jul 2011 00:00:00 GMT Sukdong Lee Gogreen PCR X3 and Gel X3

Date: Tuesday, 26 July 2011

Date Author Subject
Tue, 26 Jul 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein and TOPO
Tue, 26 Jul 2011 18:02:01 GMT Viki Ding INP PCR with Taq mix

Date: Wednesday, 27 July 2011

Date Author Subject
Wed, 27 Jul 2011 00:00:00 GMT Sukdong Lee Liquid Cultures and Colony Gogreen PCR
Wed, 27 Jul 2011 18:06:41 GMT Viki Ding INP PCR w/Taq & digestion

Date: Thursday, 28 July 2011

Date Author Subject
Thu, 28 Jul 2011 00:00:00 GMT Sukdong Lee Miniprep and Sequencing of the TOPO Silicatein

Date: Tuesday, 02 August 2011

Date Author Subject
Tue, 02 Aug 2011 18:12:35 GMT Viki Ding Ligation & transformation

Date: Wednesday, 03 August 2011

Date Author Subject
Wed, 03 Aug 2011 00:00:00 GMT Sukdong Lee Digestion of Silicatein TOPO 7

Date: Thursday, 04 August 2011

Date Author Subject
Thu, 04 Aug 2011 00:00:00 GMT Sukdong Lee Gel Extraction, Ligation, and Transformation

Date: Monday, 08 August 2011

Date Author Subject
Mon, 08 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Master Plate, Gogreen, and Gel

Date: Tuesday, 09 August 2011

Date Author Subject
Tue, 09 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep, Gogreen Liquid PCR, and Gel

Date: Wednesday, 10 August 2011

Date Author Subject
Wed, 10 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Liquid Gogreen PCR

Date: Thursday, 11 August 2011

Date Author Subject
Thu, 11 Aug 2011 00:00:00 GMT Sukdong Lee More Liquid Cultures, Liquid Gogreen PCR

Date: Friday, 12 August 2011

Date Author Subject
Fri, 12 Aug 2011 00:00:00 GMT Sukdong Lee Transformation of TOPO Silicatein Back-up

Date: Saturday, 13 August 2011

Date Author Subject
Sat, 13 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of Back-Up Silicatein TOPO

Date: Sunday, 14 August 2011

Date Author Subject
Sun, 14 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of Silicatein TOPO

Date: Monday, 15 August 2011

Date Author Subject
Mon, 15 Aug 2011 00:00:00 GMT Sukdong Lee Digest of pucBB and Silicatein TOPO

Date: Tuesday, 16 August 2011

Date Author Subject
Tue, 16 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of pucBB and Gel Extraction
Tue, 16 Aug 2011 18:14:41 GMT Viki Ding Digestion

Date: Wednesday, 17 August 2011

Date Author Subject
Wed, 17 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures of Transformants and Gogreen

Date: Thursday, 18 August 2011

Date Author Subject
Thu, 18 Aug 2011 00:00:00 GMT Sukdong Lee Gogreen PCR of Liquid Cultures

Date: Friday, 19 August 2011

Date Author Subject
Fri, 19 Aug 2011 00:00:00 GMT Sukdong Lee Gogreen PCR of Liquid Cultures

Date: Monday, 22 August 2011

Date Author Subject
Mon, 22 Aug 2011 00:00:00 GMT Sukdong Lee Ligation and Transformation
Mon, 22 Aug 2011 10:15:36 GMT Sukdong Lee Minnesota/leex4462-08222011101536-34

Date: Tuesday, 23 August 2011

Date Author Subject
Tue, 23 Aug 2011 00:00:00 GMT Sukdong Lee Ligation of pBAD+Silicatein and Digest of TOPO Silicatein
Tue, 23 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Gogreen PCR of Silicatein+pBAD

Date: Wednesday, 24 August 2011

Date Author Subject
Wed, 24 Aug 2011 00:00:00 GMT Sukdong Lee pBAD+Silicatein transformation and gel extraction of TOPO Silicatein
Wed, 24 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Digest, Transformation of pBAD

Date: Thursday, 25 August 2011

Date Author Subject
Thu, 25 Aug 2011 00:00:00 GMT Sukdong Lee Liquid Cultures, Gogreen PCR of Silicatein+pBAD
Thu, 25 Aug 2011 00:00:00 GMT Sukdong Lee Minnesota/leex4462-08252011000000-124
Thu, 25 Aug 2011 18:25:40 GMT Viki Ding Cloning #3

Date: Friday, 26 August 2011

Date Author Subject
Fri, 26 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep and Gogreen Check
Fri, 26 Aug 2011 00:00:00 GMT Sukdong Lee Minnesota/leex4462-08262011000000-125

Date: Saturday, 27 August 2011

Date Author Subject
Sat, 27 Aug 2011 18:17:00 GMT Viki Ding Cloning

Date: Monday, 29 August 2011

Date Author Subject
Mon, 29 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Digest, Transformation of pBAD
Mon, 29 Aug 2011 18:30:52 GMT Viki Ding Verifying clones

Date: Tuesday, 30 August 2011

Date Author Subject
Tue, 30 Aug 2011 00:00:00 GMT Sukdong Lee Ligation, Gel Extraction, Miniprep, Liquid Cultures of pBAD, Transformation
Tue, 30 Aug 2011 18:38:12 GMT Viki Ding SiL & GFP PCT

Date: Wednesday, 31 August 2011

Date Author Subject
Wed, 31 Aug 2011 00:00:00 GMT Sukdong Lee Miniprep of pBAD

Date: Friday, 02 September 2011

Date Author Subject
Fri, 02 Sep 2011 00:00:00 GMT Sukdong Lee PCR Overlap Extension (OmpA+Silicatein Fusion)
Fri, 02 Sep 2011 00:01:00 GMT Sukdong Lee Gel of PCR Overlap
Fri, 02 Sep 2011 00:02:00 GMT Sukdong Lee PCR Overlap Test

Date: Tuesday, 06 September 2011

Date Author Subject
Tue, 06 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Overlap
Tue, 06 Sep 2011 00:01:00 GMT Sukdong Lee PCR Overlap Extension
Tue, 06 Sep 2011 18:47:17 GMT Viki Ding Further cloning

Date: Wednesday, 07 September 2011

Date Author Subject
Wed, 07 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Products/Gel Extraction

Date: Thursday, 08 September 2011

Date Author Subject
Thu, 08 Sep 2011 00:00:00 GMT Sukdong Lee PCR Overlap Extension
Thu, 08 Sep 2011 00:01:00 GMT Sukdong Lee Gel Extraction of the PCR Overlap Extension
Thu, 08 Sep 2011 00:03:00 GMT Sukdong Lee Transformation of pBAD+ OmpA
Thu, 08 Sep 2011 00:04:00 GMT Sukdong Lee PCR of Silicatein
Thu, 08 Sep 2011 18:51:05 GMT Viki Ding More cloning

Date: Friday, 09 September 2011

Date Author Subject
Fri, 09 Sep 2011 00:00:00 GMT Sukdong Lee Part II of PCR Overlap
Fri, 09 Sep 2011 00:01:00 GMT Sukdong Lee Liquid Cultures of pBAD and pBAD+OmpA
Fri, 09 Sep 2011 00:02:00 GMT Sukdong Lee PCR of Silicatein
Fri, 09 Sep 2011 00:03:00 GMT Sukdong Lee Gel of Part2+3 and Test of Part 3

Date: Saturday, 10 September 2011

Date Author Subject
Sat, 10 Sep 2011 00:00:00 GMT Sukdong Lee Miniprep of OmpA+pBAD and pBAD
Sat, 10 Sep 2011 00:01:00 GMT Sukdong Lee Gel Extraction of Part 3
Sat, 10 Sep 2011 00:03:00 GMT Sukdong Lee Gel of PCR Silicatein
Sat, 10 Sep 2011 00:04:00 GMT Sukdong Lee PCR Overlap Check

Date: Monday, 12 September 2011

Date Author Subject
Mon, 12 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR Products
Mon, 12 Sep 2011 00:01:00 GMT Sukdong Lee PCR of Part 3 and Silicatein
Mon, 12 Sep 2011 18:54:16 GMT Viki Ding Verifying clones

Date: Tuesday, 13 September 2011

Date Author Subject
Tue, 13 Sep 2011 00:00:00 GMT Sukdong Lee PCR of Part 3

Date: Wednesday, 14 September 2011

Date Author Subject
Wed, 14 Sep 2011 00:00:00 GMT Sukdong Lee Gel of PCR

Date: Friday, 16 September 2011

Date Author Subject
Fri, 16 Sep 2011 00:00:00 GMT Sukdong Lee PCR of Silicatein, part 1, part S, part 2, and part 3
Fri, 16 Sep 2011 00:01:00 GMT Sukdong Lee Gel of PCR Products+Extraction
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