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  • Team:OUC-China/Result/date
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> ...H2O</span></p></td></tr><tr class="c12"><td class="c1"><p class="c4"><span>6</span></p></td><td class="c1"><p class="c4"><span>4</span></p></td><td clas
    146 KB (27,225 words) - 02:34, 29 October 2011
  • Team:Hong Kong-CUHK/Notebook
    <div class="span-6 square"> ...-size:20.0pt;">Week 1 </span></b><b><span style="font-size:10.0pt;">Monday 6 June &ndash; Sunday 12 June</span></b></div>
    1.35 MB (148,320 words) - 17:48, 28 October 2011
  • Team:Hong Kong-CUHK/Laboratory/log book
    <div class="span-6 square"> ...-size:20.0pt;">Week 1 </span></b><b><span style="font-size:10.0pt;">Monday 6 June &ndash; Sunday 12 June</span></b></div>
    1.35 MB (148,301 words) - 15:24, 5 October 2011
  • Team:NTNU Trondheim/Journal
    == Thursday 23/6 == == Friday 24/6 ==
    64 KB (10,273 words) - 15:51, 21 September 2011
  • Team:Hong Kong-CUHK/Laboratory log book
    <div class="span-6"> <div class="span-6 square">
    278 KB (35,620 words) - 04:07, 3 October 2011
  • Team:BU Wellesley Software/Notebook/KyleNotebook
    ...helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>WEEK 1: 6/6-6/10/2011</h5> 6/6: Monday was our last day of "boot camp". With the comp. team and Wellesley
    50 KB (8,331 words) - 22:49, 28 September 2011
  • Team:Potsdam Bioware/Labjournal/September part 1
    *** clone 6 *** clone 6 -
    151 KB (21,501 words) - 17:06, 21 September 2011
  • Team:Michigan/Notebook
    ==Week 4 (5/29/2011-6/4/2011)== ⇒ 6 microliters of DNA ladder was used without dilution; this may be a bad idea
    73 KB (11,794 words) - 05:23, 29 September 2011
  • Team:METU-Ankara/slider.js
    ...6(3.j==\'1v\'||4.r==\'1v\'){b i=0;$(\'.7-c\',5).H(9(){$(g).e(\'y\',\'s%\');6(i==3.h-1){$(g).C({z:\'1.0\'},(3.o*2),\'\',9(){5.16(\'7:X\')})}k{$(g).C({z:\
    7 KB (1,589 words) - 09:52, 20 September 2011
  • Team:MIT/Notebook/
    <li id="w6">Week 6</li> ...another university, worked throughput the summer, while a smaller group of 6 students continued beyond August as UROPs.
    131 KB (18,449 words) - 03:35, 29 October 2011
  • Team:Potsdam Bioware/Labjournal/August part 2
    * pSB1T3+YFP II clone a: 62.6 ng/µl * pSB1T3+YFP II plate 2 clone d: 25.6 ng/µl
    134 KB (19,391 words) - 13:03, 21 September 2011
  • Team:KAIST-Korea/js/fancybox/jquery easing
    ...-=(2.18/2.k))*t+.19)+b}},1b:9(x,t,b,c,d){e(t<d/2)6 h.i.A(x,t*2,0,c,d)*.5+b;6 h.i.v(x,t*2-d,0,c,d)*.5+c*.5+b}});',62,74,'||||||return||Math|function|||||
    7 KB (1,308 words) - 22:43, 11 August 2011
  • Team:SYSU-China/temp/menu.js
    ...t-=(2.16/2.k))*t+.11)+b}},Z:9(x,t,b,c,d){e(t<d/2)6 h.j.A(x,t*2,0,c,d)*.5+b;6 h.j.w(x,t*2-d,0,c,d)*.5+c*.5+b}});',62,74,'||||||return||Math|function||||| ...,d)},g:3(x,t,b,c,d){2 0.1.m(x,t,b,c,d)},o:3(x,t,b,c,d){2 0.1.e(x,t,b,c,d)},6:3(x,t,b,c,d){2 0.1.5(x,t,b,c,d)},4:3(x,t,b,c,d){2 0.1.a(x,t,b,c,d)},9:3(x,t
    18 KB (3,260 words) - 07:03, 5 October 2011
  • Team:Johns Hopkins/Notebook/Vio
    =====6/11/2011===== ...d0616d1ee32e93fce3dfa8a7e6624c4.media.640x480 (1).jpg|thumb|200px|Vio UTRs 6/11/11]]
    78 KB (11,015 words) - 22:10, 28 October 2011
  • Team:UNAM-Genomics Mexico/Templates/Parts
    ...o/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> ...co/Project/HydrogenProduction"><span>Hydrogen Production</span><![if gt IE 6]></a>
    40 KB (5,411 words) - 17:59, 21 September 2011
  • Team:Potsdam Bioware/Labjournal/August part 1
    * 6 µl (ca 70 ng) digested vector pARW089 (NarI, AatII) *Template: 1µl = 3,6 ng
    117 KB (17,234 words) - 01:03, 22 September 2011
  • Team:BU Wellesley Software/Notebook/JanooNotebook
    *<del>read through Friedland abstract/paper</del> [6/7/2011] *<del>read through Puppeteer/PuppetShow powerpoint</del> [6/7/2011]
    48 KB (7,116 words) - 22:00, 27 September 2011
  • Team:UNITS Trieste/jquery.qtip-1.0.0-rc3.min.js
    ...!`%-*79992` 6.`!O5red`!b!g`4^!`!E@A9DB66`!Q4b9db8c`!6'58792`'N!` ;)CDE6AC` 6.`!P5`!\\!`!f!blue`!DBDD9ED`!R4D0E9F`&G!`!:$E99B` J!` ;)E5F6F`!T!`&}$D9FB`#8
    22 KB (3,202 words) - 14:47, 11 June 2011
  • Team:UNAM-Genomics Mexico/Templates/Modeling
    ...o/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> ...co/Project/HydrogenProduction"><span>Hydrogen Production</span><![if gt IE 6]></a>
    13 KB (1,704 words) - 01:42, 29 September 2011
  • Team:UNAM-Genomics Mexico/Templates/Sliderbiosintetizarte
    ...o/Project"><span>Project</span><![if gt IE 6]></a><![endif]><!--[if lte IE 6]><table><tr><td><![endif]--> ...co/Project/HydrogenProduction"><span>Hydrogen Production</span><![if gt IE 6]></a>
    15 KB (1,822 words) - 01:57, 29 September 2011
  • Team:Debrecen Hungary/Protocols
    6) Close it and stick a tape from one side of the wall vertically over the ca 6. Spread the transformed bacteria on the Petri dishes with LB agar and the a
    51 KB (7,937 words) - 05:49, 22 September 2011
  • Team:WHU-China/calendarjs
    ...nction Fa(a,b,e){for(b=b||1;!a.getDay()||e&&a.getDay()==1||!e&&a.getDay()==6;)ba(a,b);return a}function Ca(a,b){return Math.round((N(a,true)-N(b,true))/ ...)%7);Q=Math.round((S-y)/(Ab*7));if(f("weekMode")=="fixed"){ba(S,(6-Q)*7);Q=6}d.title=l(j,
    72 KB (13,661 words) - 13:53, 24 October 2011
  • Team:Penn/project/protocols/
    ...='http://benjaminshyong.com/igem2011/wp-includes/js/jquery/jquery.js?ver=1.6.1'></script> 6. Add 1.5 mL trypsin to the bottom of the flask. Move the flask back and for
    86 KB (13,981 words) - 04:46, 29 September 2011
  • Team:OUC-China/Result/week12
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> ...re used for standerdizing 18-3-11,14-19,5-4,rhiABCpro,13-6-leu and 18-3-11-6-leuB have already bacteria. And we select some into LB.</p>
    7 KB (1,143 words) - 02:38, 29 October 2011
  • Team:Potsdam Bioware/Labjournal/September part 2
    * 6 overnight cultures | mdnABCDE with T7-promotor clone 1 || 50.6
    58 KB (8,180 words) - 00:26, 22 September 2011
  • Team:TU Munich/lab/notebook/part3
    ...dinburgh part") conc: 150 ng/µl</p><p>primers (synthesized by biomers, 29.6.11, stock: 100µM):</p> ...iPrep iGEM R2 31.5.11) was digested with X,P and T7prom (black writing: 17.6.11 T7) with S,P. After heat-inactivation, GLP was added to the digested DNA
    121 KB (20,136 words) - 01:54, 22 September 2011
  • Team:WashU/Notebook/June2011
    == June 6 == **[[File:IGEM Practice Gel 6-9.jpg|thumb|none|upright=3]]
    26 KB (4,070 words) - 16:08, 25 July 2011
  • Team:Harvard/Template:NotebookData4
    *these were the only two PCR reactions from 6/22/11 to work **omega: 6.1ng/µL, 260/280=1.83
    31 KB (4,866 words) - 16:56, 8 August 2011
  • Team:WHU-China/Notebook/Daytoday
    <span style="font-weight:bolder">5/29~6/1</span>, use Pst1 and EcoRⅠto digest pDsRed-Express Vector</br> <span style="font-weight:bolder">6/2~6/15</span>, produce the competent cell, use T4 ligase to join DNA fragments,
    29 KB (3,834 words) - 14:34, 26 October 2011
  • Team:Rutgers/LB1
    <td colspan="6" td background="https://static.igem.org/mediawiki/2011/9/96/Stripe.png"><h1 <td colspan="6" class="stuff">
    62 KB (8,401 words) - 02:39, 29 September 2011
  • Team:TU Munich/lab/notebook/part4
    <p>6: pSB4A5, c = 175 ng/µl</p> <td>6: BBa_K568001 </td><td><span style="color:#FFAA00">6.8 kbp</span> </td><td>2 kbp </td><td>E,P
    78 KB (13,436 words) - 22:42, 28 October 2011
  • Team:Harvard/Template:NotebookData2
    **Bands followed the same pattern as the gel run on 6/13/11 **OZ052 (from 1:100 dilution): 20.6 ng/µL, 260/280=2.17
    26 KB (3,991 words) - 23:44, 25 October 2011
  • Team:NCTU Formosa/calendar
    ...ckbone to 1C3 5. extract plasmid of D circuit and check by electrophoresis 6. incubated (B0034+ilvC) (B0030+ilvD) (B circuit 7-1) (K098995 +B0034) (vioA
    46 KB (7,856 words) - 13:40, 5 October 2011
  • Team:BU Wellesley Software/Notebook/SiruiNotebook
    *'''5/31-6/1''': Team introductions; we learned the basics of CAD and basics of molecu *'''6/2''': We started learning the basics of Clotho, as well as more basic biolo
    29 KB (4,439 words) - 03:57, 29 September 2011
  • Team:BU Wellesley Software/Notebook/ChenkaiNotebook
    *'''Wednesday 6/8''' <h2>Thursday, 6/9</h2>[[File: RMPuppeteerCommands 6-9-11.png |300px|thumb|right|New Puppeteer output with UUIDS replacing sampl
    28 KB (4,611 words) - 16:34, 27 September 2011
  • Team:Harvard/Template:NotebookData
    *6) 68⁰C for 5 min ...hould have been 1200 µL bacteria media, 300 µL 80% glycerol ''(Corrected 6/14/2011)'' ''' (80% pure glycerol, 20% molecular grade water)
    14 KB (1,886 words) - 14:52, 12 August 2011
  • Team:Grinnell/Notebook/Gels/Esp
    ...> ligated with <i>rsaA</i> C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i> C-terminal. ...sted 20110606; lane 5: PCR product of plasmid containing <i>esp</i>; lanes 6-8: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated wi
    32 KB (4,737 words) - 21:32, 1 August 2011
  • Team:Harvard/Template:NotebookDataJuly
    ... We grew up 12 colonies from each 6/29 plate and 6 "colonies" from the 2mL 6/30 plate in SOC medium with kanamycin, and next week we will PCR the 152962 **Used KAPA master mix and protocols, same procedure as 6/29/11
    9 KB (1,439 words) - 18:13, 3 August 2011
  • Team:Potsdam Bioware/Labjournal/June
    | Primer (each) || 3 || 0.6 µM | 6 || || Hold
    70 KB (9,678 words) - 01:31, 22 September 2011
  • Team:Grinnell CN/Notebook/Gels/Esp
    ...> ligated with <i>rsaA</i> C-terminal PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i> C-terminal. ...sted 20110606; lane 5: PCR product of plasmid containing <i>esp</i>; lanes 6-8: PCR product using VF2 and VR of plasmid containing <i>esp</i> ligated wi
    32 KB (4,738 words) - 04:14, 27 September 2011
  • Reporter: Week 6 June 20-25
    ... of growth in the cultures is the entire colony was used for colony PCR on 6/17, leaving no cells to grow in culture. In order to grow more cells in cul ...n. The purified tev protease PCR product from 6/4 and the K3 miniprep from 6/13 were digested with XbaI and PstI in buffer 3. The digests were ligated t
    8 KB (1,358 words) - 17:27, 13 July 2011
  • Tianjin/script 1
    ...rguments.length){case 4:return new c.matrix.M2x2(e[0],e[2],e[1],e[3]);case 6:return new c.matrix.M3x3(e[0],e[2],e[4],e[1],e[3],e[5],0,0,1)}},reflect:fun
    20 KB (3,874 words) - 02:46, 7 September 2011
  • Team:KAIST-Korea/Notebook/Week1
    ...Notebook/Week1"><img id="tag3" src="https://static.igem.org/mediawiki/2011/6/64/Kaist2011_text_lab_diary.png" /> |[[Team:KAIST-Korea/Notebook/Week6|Week 6]]
    10 KB (1,492 words) - 13:12, 20 August 2011
  • Team:UNIPV-Pavia/Measurements
    ... class="tocnumber">2</span> <span class="toctext">Measuring 3OC<sub><small>6</small></sub>-HSL synthesis and degradation</span></a> ...g"><span class="tocnumber">2.3</span> <span class="toctext">3OC<sub><small>6</small></sub>-HSL degradation in M9 medium and cultures not expressing lact
    19 KB (3,055 words) - 00:40, 22 September 2011
  • Team:BU Wellesley Software/Notebook/EvelynNotebook
    ...y: helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h3>6/1/2011-6/11/2011</h3> ...y: helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h3>6/6/2011-6/10/2011</h3>
    29 KB (4,664 words) - 00:38, 29 September 2011
  • Team:BU Wellesley Software/Notebook/CraigNotebook
    6. <span style="text-decoration:line-through;">Design first version of final <h4> 6/4/11 </h4> - Today I learned how to make a basic GUI in java and use the te
    38 KB (6,388 words) - 05:18, 28 September 2011
  • Team:WashU/Notebook/June
    ===June 6=== *6.25 uL dNTP
    30 KB (4,553 words) - 17:55, 19 July 2011
  • Team:KAIST-Korea/Notebook/Week2
    ...Notebook/Week1"><img id="tag3" src="https://static.igem.org/mediawiki/2011/6/64/Kaist2011_text_lab_diary.png" /> |[[Team:KAIST-Korea/Notebook/Week6|Week 6]]
    8 KB (1,254 words) - 13:12, 20 August 2011
  • Team:Penn/notebook/
    ...='http://benjaminshyong.com/igem2011/wp-includes/js/jquery/jquery.js?ver=1.6.1'></script> <h1>6/1/11</h1>
    70 KB (11,208 words) - 04:45, 29 September 2011
  • Team:KAIST-Korea/Notebook/Week3
    ...Notebook/Week1"><img id="tag3" src="https://static.igem.org/mediawiki/2011/6/64/Kaist2011_text_lab_diary.png" /> |[[Team:KAIST-Korea/Notebook/Week6|Week 6]]
    7 KB (1,006 words) - 13:10, 20 August 2011
  • RecA: Week 6, June 19-24
    ... and these digests were run on a 1% agarose gel. The old RecA digests from 6/17 were also ran on the gel. The terminator (B0015) and the PcI reporter (I ===RecAI Extraction Take 6, Day 1===
    5 KB (773 words) - 16:36, 3 July 2011
  • RecA: Week 6, June 19-25
    ... and these digests were run on a 1% agarose gel. The old RecA digests from 6/17 were also ran on the gel. The terminator (B0015) and the PcI reporter (I ===RecAI Extraction Take 6, Day 1===
    5 KB (783 words) - 03:01, 29 September 2011
  • Team:Potsdam Bioware/Labjournal/July
    | 1 || mdnA with SfiI || 6 || 224 * 6 µl DNA Ladder Gene Ruler (1:10) <br>
    55 KB (8,148 words) - 21:15, 18 September 2011
  • Team:Harvard/Project/Design
    ...ion in a backbone. Amino acid positions are specified by -1,1,2,3,4,5, and 6. ...NA triples. We took frequencies of each amino acid in each position (-1 to 6) in each of 12 DNA triplet types (ANN, NAN, NNA, etc, where N is any of the
    26 KB (3,950 words) - 12:55, 28 October 2011
  • Team:Michigan/Human Practices
    ==6/8/11== June: Finish interviews (6/8) and conduct them until end of June. Then gather data. Board Game – wor
    7 KB (1,156 words) - 14:39, 27 September 2011
  • Team:Uppsala-Sweden/unicodemenus.js
    awmCF(6,1,1,-10,0); it=s0.addItemWithImages(6,7,n,"Project",n,"","","https://2011.igem.org/Team:Uppsala-Sweden/Project",n
    15 KB (2,315 words) - 23:44, 12 July 2011
  • Team:British Columbia/Model1
    ...o 2 sources: “Writing Differential Equations” '''[2]''' and “Chapter 6- Transport and Kinetics” '''[3]'''. However, many constants and concentra ...instead of 75% FPP and 25% GPP" as compared to the wildtype ERG20 gene '''[6]'''. We want more GPP than FPP because our goal is to produce monoterpenes
    22 KB (3,354 words) - 02:42, 28 October 2011
  • Script
    ...142r-33,25r-28,-40r-30,41r-32,-26r31,-34r-42,-15r15,-35r41,19r-5,-45r44,0r-6,46r41,-20r15,35r-44,15","w":148},"^":{"d":"82,-252r52,0r77,143r-64,0r-39,-8
    34 KB (6,310 words) - 02:45, 7 September 2011
  • Team:Bielefeld-Germany/Labjournal
    ==Week 6: 6th - 12th June== ... in LB medium were induced adding 0,1 % L-rhamnose at OD<sub>600</sub> = 0,6.''']]
    32 KB (4,418 words) - 23:09, 28 October 2011
  • Team:Edinburgh/Lab Notebook
    '''June 6:''' ... some were white). Six blue colonies were selected, replated and grown for 6 hours on the media mentioned above. Some cells were then transferred to bot
    69 KB (11,148 words) - 15:33, 3 October 2011
  • Team:BU Wellesley Software/Notebook/VanessaNotebook
    ...helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h3>Week 1: 6/1-6/10</h3> ...elvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h3>Week 2: 6/11-6/17</h3>
    25 KB (3,928 words) - 02:07, 28 September 2011
  • Team:Colombia/Notebook
    ===July 6=== * Bricks 2, 5, 6,7, 8, 9 and 10 were transformed and plated.
    10 KB (1,441 words) - 18:10, 27 September 2011
  • Team:BU Wellesley Software/Notebook/ShannonNotebook
    ...elvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>WEEK 1: 6/06-6/10/2011</h5> ...elvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>WEEK 2: 6/13-6/17/2011</h5>
    20 KB (2,751 words) - 01:27, 29 September 2011
  • Team:Peking S/lab/notebook/shd
    <font size=6> <font color="#FFFFFF">Handuo Shi's Notebook</font></FONT> |style="text-align:center"| 6
    19 KB (2,592 words) - 05:05, 5 October 2011
  • Team:Imperial College London/Project/Auxin/Results
    ...ith pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also transformed the cells with part BBa_K316001 to produce cell line 7 ...he experiments that were conducted on this day were a mini-prep on samples 6,7,8 and 10 that were inoculated into an LB broth culture the night before.
    24 KB (4,082 words) - 16:03, 12 September 2011
  • Team:TU Munich/lab/notebook/part1
    ...EM10_Edinburgh </td><td>Submissions </td><td>Shipment: 00694 </td><td>6 </td><td>pSB1C3 </td><td>C 6 µl each lane
    49 KB (7,813 words) - 02:39, 22 September 2011
  • Team:Harvard/Bioinformatics
    ...ion in a backbone. Amino acid positions are specified by -1,1,2,3,4,5, and 6. if(k==6){
    16 KB (2,492 words) - 02:34, 24 September 2011
  • Team:Grenoble/Projet/Modelling/Results
    The following simulation was realized for an IPTG gradient of $1x10^{-6} M$ to $1x10^{-2} M$ and an aTc concentration of $5x10^{-6} M$. The first graph present the logarithmic IPTG gradient in
    35 KB (4,885 words) - 11:16, 25 October 2011
  • Team:Harvard/Project/Bioinformatics
    ...ion in a backbone. Amino acid positions are specified by -1,1,2,3,4,5, and 6. if(k==6){
    22 KB (3,444 words) - 03:40, 29 September 2011
  • Team:KIT-Kyoto/GFP-MLF実験９月かんたん
    <tr><td align=center>10 x H Buffer</td><td align=right>6 µl</td></tr> 9/6(火)★おｋ★<BR>
    71 KB (8,179 words) - 01:41, 6 October 2011
  • Team:Hong Kong-CUHK/Laboratory/protocol
    <div class="span-6 square"> 1.6 Gel Extraction
    27 KB (4,356 words) - 18:57, 28 October 2011
  • Team:Virginia Tech/Notebook
    ==Week of 6/6/2011== ==Week of 6/13/2011==
    10 KB (1,606 words) - 03:56, 29 September 2011
  • Team:BU Wellesley Software/Notebook/AlbertoNotebook
    6. I13458_I13453_E0240 (double cut) 6. I0500_J23100 (double cut)
    51 KB (8,151 words) - 05:01, 29 September 2011
  • Team:BU Wellesley Software/Notebook/TraciNotebook
    ...helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>WEEK 1: 6/6-6/10/2011</h5> 6. Repeat 1-5 until transcriptional units are made
    15 KB (2,198 words) - 18:27, 27 September 2011
  • Team:BU Wellesley Software/Notebook/JenhanNotebook
    <h5>'''(6/3)'''</h5> Today was the Clotho boot camp. Trying to build an application <h5>'''(6/7)'''</h5> Still quite a bit confused about what everyone is doing. Chenka
    21 KB (3,180 words) - 03:55, 29 September 2011
  • Team:NTNU Trondheim/Journal Modeling
    == Wednesday 22/6 == ==Friday 24/6==
    9 KB (1,376 words) - 15:24, 20 September 2011
  • Reporter: Week 5 June 12-17
    ...we transformed the Imp linker + K3 miniprep made for sequencing on Friday, 6/10 into Escherichia coli cells and plated onto kanamycin resistant plates. ... on [[Reporter:_Week_4_June_6-11#Mutagenesis of Xyle, Take 3 Day 1|Friday, 6/10]], was performed.
    10 KB (1,638 words) - 17:26, 13 July 2011
  • Team:UNIPV-Pavia/Calendar/September/week2
    ...he moment of induction, named t = 0 h and then at t = 2 h, t = 4 h and t = 6 h (also from two negative controls, ENTERO4C5 and M9). ...ll microplate and induced with 10 &mu;l of supernatants and 3OC<small><sub>6</sub></small>-HSL known concentrations.
    13 KB (2,252 words) - 10:17, 18 September 2011
  • Team:UC Davis/Notebook wip
    --Monday 6/13/11-- --Tuesday 6/14/11--
    15 KB (2,467 words) - 19:25, 18 July 2011
  • Team:Nevada/Notebook/Temp
    <font size=6>Temp Calender</font> <li>1. glucose + ATP --Hx-> G-6-P + ADP</li>
    8 KB (1,175 words) - 18:50, 4 August 2011
  • Team:Harvard/Template:NotebookDataAugust3
    ***numbered, from 1-6, 1-3 is colony 1, 4-6 is colony 2. 1,4=TT 2,5=RR, 3,6=ZR ====Sequencing results for oligos 5 and 6====
    23 KB (3,770 words) - 22:30, 19 September 2011
  • Team:Harvard/Template:NotebookDataAugust4
    ...Nida and I combined the miniprepped products (everything except for sample 6, which had very low miniprep yields for some reason) in equal molar ratios *6: 10^-4
    31 KB (5,048 words) - 23:43, 25 October 2011
  • Team:OUC-China/Result/week11
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> <p>Enlargement culture the 6&leu ligated in 3A method at 11 a.m. and coat them on the square plates then
    6 KB (955 words) - 02:38, 29 October 2011
  • Team:Imperial College London/Project/Auxin/Assembly
    ...ith pSB1C3 containing BBa_K398500 and J23100 promoter to produce cell line 6. We also transformed the cells with part BBa_K316001 to produce cell line 7 ...he experiments that were conducted on this day were a mini-prep on samples 6,7,8 and 10 that were inoculated into an LB broth culture the night before.
    12 KB (2,194 words) - 10:17, 14 September 2011
  • Team:uOttawa/Results
    ...''Figure 1.''' Construct created by BrickMason assembly. It is composed of 6 parts. Constructs 1-6 were PCRed using biobrick primers and then cut out of a gel, This is shown
    8 KB (1,324 words) - 06:49, 27 October 2011
  • Team:Harvard/Template:NotebookDataAugust2
    *To get more pSB4K5 3:11P we did a miniprep of a 6 mL culture #Discard EtOH, let tubes dry for approximately 6-8 minutes by inverting them on a piece of Kimwipe tissue.
    23 KB (3,737 words) - 22:45, 19 September 2011
  • Team:BYU Provo/Team Thermosensor/Week10
    ===6/21/11=== ===6/22/11===
    2 KB (305 words) - 08:21, 24 September 2011
  • Team:Bielefeld-Germany/Results/S-Layer/CspB CH
    ...ing KRX wildtype is shown for comparison. After induction at approximately 6 h the OD<sub>600</sub> of the induced K525222 visibly drops when compared t ...ing KRX wildtype is shown for comparison. After induction at approximately 6 h the RFU to OD<sub>600</sub> ratio starts to rise in the induced culture.
    29 KB (4,235 words) - 03:16, 29 October 2011
  • Team:Arizona State/Project/References
    ...ssociated with DNA repeats in prokaryotes,” Mol Microbiol., vol. 43, no. 6, March 2002.</div> ...s Exist in Prokaryotic Genomes,” PLoS Computational Biology, vol. 1, no. 6, pp. 474-483, 1 November 2005.</div>
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  • Team:BU Wellesley Software/Notebook/MargauxNotebook
    !colspan="6"|Protocol List ...y: helvetica, arial, sans-serif;font-size:1em;color:#ea8828;"|<h5>6/1/11-6/6/11:BU-WELLESLEY TEAM BOOT CAMP</h5>
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  • Team:Caltech/Week 3
    <td>205.6</td> ...t:BBa_K123000 K123000]; 5 [http://partsregistry.org/Part:BBa_B0014 B0014]; 6 [http://partsregistry.org/Part:BBa_R0040 R0040]; 7 [http://partsregistry.or
    9 KB (1,375 words) - 23:15, 11 July 2011
  • Team:uOttawa/ResultsTemp
    ...''Figure 1.''' Construct created by BrickMason assembly. It is composed of 6 parts, the parts will be referred to by the red numbers shown above them. Constructs 1-6 were PCRed using biobrick primers and then cut out of a gel, This is shown
    6 KB (974 words) - 02:43, 29 September 2011
  • Team:Freiburg/Notebook/18 August
    Name of the parts: GFP-pbd 4 4 4, GFP-pbd 4 1 3, GFP-pbd 6 6 8, GFP-pbd 4 2 2 GFP-pbd 6 6 8: 163,5 ng/µl
    12 KB (1,424 words) - 01:09, 22 September 2011
  • Team:KIT-Kyoto/matsunami
    <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr> <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr>
    48 KB (6,897 words) - 04:27, 30 September 2011
  • Team:Potsdam Bioware/Labjournal/October
    | 6 | 32.6
    89 KB (13,149 words) - 18:03, 28 October 2011
  • Team:Grinnell/Notebook/Gels/DspB
    ...mation_results_colonies_1_2_3' src='https://static.igem.org/mediawiki/2011/6/6d/20110707_UBC_dspB_transformation_results_colonies_1_2_3.jpg' /> ...: with BBa_K081005 colonies 3 and 4; lane 5: standard WT <i>dspB</i>; lane 6: ladder.
    11 KB (1,699 words) - 14:10, 5 August 2011
  • Team:KIT-Kyoto/Notebook/LabNote/GFPMLF9
    <tr><td align=center>10 x H Buffer</td><td align=right>6 µl</td></tr> :<tr><td align=center>10 x H Buffer</td><td align=right>6 µl</td></tr>
    37 KB (6,399 words) - 03:45, 6 October 2011
  • Team:Johns Hopkins/Notebook/YTExperiments
    *.6µl Forward Primer *.6µl Reverse Primer
    29 KB (4,167 words) - 03:47, 29 September 2011
  • Team:Grinnell CN/Notebook/Gels/DspB
    ...mation_results_colonies_1_2_3' src='https://static.igem.org/mediawiki/2011/6/6d/20110707_UBC_dspB_transformation_results_colonies_1_2_3.jpg' /> ...: with BBa_K081005 colonies 3 and 4; lane 5: standard WT <i>dspB</i>; lane 6: ladder.
    11 KB (1,700 words) - 04:14, 27 September 2011
  • Team:SJTU-BioX-Shanghai/Project/Subproject1/Modeling
    ... 1 represents sulA promoter-tRNA<sup>Arg</sup>) and Pbla-Luc-nAGG (n=2, 4, 6, 8) system. This summarizes the modulating stimulated by ultra-violet ray]] ...esents ''sulA'' promoter-tRNA<sup>Arg</sup> and P''bla''-Luc-nAGG (n=2, 4, 6, 8) system. This summarizes the modulating stimulated by ultra-violet ray
    9 KB (1,406 words) - 18:47, 28 October 2011
  • Team:SJTU-BioX-Shanghai/Notebook/Lablog1
    ==8/6/2011== ddH2O 6.2ul
    31 KB (4,713 words) - 15:15, 5 October 2011
  • Team:Amsterdam/Header
    <!--[if lte IE 6]><table><tr><td><![endif]--> <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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  • Team:Caltech/Protocols
    2. Also add 6 ul H2O.<br/> 6. Add CIP to the backbone digest. Incubate at 37˚C for 1 hour<br/>
    8 KB (1,283 words) - 23:00, 28 September 2011
  • RecA: Week 5, June 13-18
    ===RecAI Mutagenesis Test, Take 2 Day 6=== &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The assembly from 6/13 was plated early in the morning in order for the colonies to grow all da
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  • Team:Waterloo
    <b>Figure 6</b> represents the positive control. For consistency, GFP1 and GFP2 are sep ...ribes the resultant bands from the diagnostic digestion. Although bands 5, 6 and 7 (sub clones) have been confirmed, the adjacent positive control (band
    132 KB (19,352 words) - 19:19, 28 October 2011
  • Team:UNIPV-Pavia/Team
    nam[6] = "Edoardo Baldini"; port[1] = "https://static.igem.org/mediawiki/2011/6/6f/Niccolofranceschi.jpg";
    23 KB (2,804 words) - 13:16, 25 November 2011
  • Team:KAIST-Korea/Notebook/Week4
    ...Notebook/Week1"><img id="tag3" src="https://static.igem.org/mediawiki/2011/6/64/Kaist2011_text_lab_diary.png" /> |[[Team:KAIST-Korea/Notebook/Week6|Week 6]]
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  • Team:Harvard/Template:NotebookData3
    ***Have 3-6 target sequences (we're currently going for 8) **Proposed: 3 diseases, 6 sequences
    27 KB (3,941 words) - 22:02, 20 September 2011
  • Team:Freiburg/Notebook/31 August
    ... mM dCTP300 μl of 1 M DTT 1.5 g PEG-8000300 μl of 100 mM NADAdd water to 6 ml Aliquot 100 μl and store at -20 °C ...er mixture. The DNA should be in equimolar amounts. Use 10-100 ng of each ~6 kb DNA fragment. For larger DNA segments, increasingly proportionate amount
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  • Team:Valencia/Notebook/August
    | &nbsp;6 || &nbsp;7 || &nbsp;8 || &nbsp;9 || 10 || 11 || 12 | &nbsp;4 || &nbsp;5 || &nbsp;6 || &nbsp;7 || &nbsp;8 || &nbsp;9 || 10 ||
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  • Team:Amsterdam/Header2
    <!--[if lte IE 6]><table><tr><td><![endif]--> <!--[if lte IE 6]></td></tr></table></a><![endif]-->
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  • Team:UNIPV-Pavia/TestTeam
    nam[6] = "Edoardo Baldini"; port[1] = "https://static.igem.org/mediawiki/2011/6/6f/Niccolofranceschi.jpg";
    22 KB (2,731 words) - 22:20, 15 September 2011
  • Team:TU Munich/model/combineddata
    ...cy in the parameters.</div><h2 class='section'><span class='section_label'>6</span> <a id='magicparlabel-12443' ></a>conclusion</h2><div class='standard
    100 KB (14,899 words) - 11:42, 28 October 2011
  • Team:TU Munich/model/redlightdata
    ...ear reaching a limit.</div><h2 class='section'><span class='section_label'>6</span> <a id='magicparlabel-26809' ></a>Conclusion</h2><div class='standard
    48 KB (7,341 words) - 11:33, 28 October 2011
  • Team:Bielefeld-Germany/SinceAmsterdam
    ...GEM-Bielefeld2011-Ecoligrowthsimple.jpg|center|220px]] <div align="right">(6)</div> Figures 6-10 show a comparison between modeled and measured data for cultivations wit
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  • Team:KIT-Kyoto/DIAP2-MALT実験
    <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr> <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr>
    54 KB (7,329 words) - 09:21, 4 October 2011
  • Team:Penn/results/ivis-imaging/
    ...='http://benjaminshyong.com/igem2011/wp-includes/js/jquery/jquery.js?ver=1.6.1'></script> ...spatial resolution from the scale of one well in a 6-well plate, to a full 6-well plate. The Small Animal Imaging Facility (SAIF) at Penn was very gener
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  • Team:SYSU-China/page project notes
    ...0. (1~4 are trkd-pET-32a induced by 0, 0.1, 0.5, 1.0 mM IPTG, 5 is marker, 6~9 are cheZ-pET-32a induced by 0, 0.1, 0.5, 1.0 mM IPTG). </P> <br /> <li>Sept.6, 2011 Tuesday</li>
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  • Team:Johns Hopkins/mootoolsjs
    ...o br=(1D-75)/4p;B(5W==1D)2W=br-8W;14 B(65==1D)2W=2+8l-br;14 2W=4+8W-8l;2W/=6;B(2W<0)2W++}m[1c.2q(2W*bc),1c.2q(6y*35),1c.2q(8k*35)]},b9:k(){o br=1c.2q(c[
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  • Team:Lethbridge/Notebook/Lab Work/Group1
    **Picked 6 colonies from Enhanced Lumazine synthase plates from August 12 *6) pBad- P0440 in pSB1A2 cut at EcoRI and XbaI
    22 KB (3,366 words) - 03:32, 29 September 2011
  • Team:NTNU Trondheim/Team
    |valign="top" rowspan="6"|[[File:Anders.png|150px|Anders Halgunset]] |valign="top" rowspan="6"|[[File:Gunvor2.png|150px|Gunvor Røkke]]
    13 KB (1,979 words) - 13:50, 21 September 2011
  • Team:Grinnell/Notebook
    ...g <i>esp</i> ligated with <i>rsaA</i> PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i>.</li> ===Week 6 July 3-9===
    24 KB (3,886 words) - 03:49, 29 September 2011
  • Reporter: Week 3 May 30- June 4
    |4:1 ratio of insert to vector<br /> 6:1 ratio of insert to vector To get a 6:1 ratio of insert DNA to vector DNA, the following changes were made to the
    9 KB (1,317 words) - 17:23, 13 July 2011
  • Reporter: Week 4 June 6-11
    ==Monday, June 6== |5 ml of SOB media with 6 &#956;l of chloramphenicol<br />was innoculated with the Imperial linker+C3
    10 KB (1,545 words) - 17:24, 13 July 2011
  • Reporter: Week 7 June 26-July 1
    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The plates containing the assembly from 6/25 grew colonies that only contained RFP, none of which contained the tev p ...sp;The purified tev protease PCR product from 6/4 and the K3 miniprep from 6/25 were digested with XbaI and PstI in buffer 3. The digests were ligated t
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  • Team:Arizona State/Notebook/June
    == Monday, June 6 == :* From: 6-2 plate MG1655
    12 KB (1,827 words) - 02:05, 29 September 2011
  • Team:Bielefeld-Germany/Backup-Protocols
    * assembly mixture (6 mL): ** 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm)
    62 KB (8,860 words) - 12:04, 15 September 2011
  • Team:Harvard/Test Page 2
    #headerlinktable #vision a { background:url('/wiki/images/6/63/GroningenBarVisionLight.png'); } #headerlinktable #protocols a { background:url('/wiki/images/6/65/GroningeBarProtocolsLight.png'); }
    36 KB (4,536 words) - 18:48, 7 July 2011
  • Team:NTNU Trondheim/Lag
    |valign="top" rowspan="6"|[[File:Bilde1.png]] |valign="top" rowspan="6"|[[File:Bilde1.png]]
    5 KB (671 words) - 08:46, 12 August 2011
  • Team:Nevada/Notebook/Weeks14
    <font size=6>Calender Weeks 1-4</font> <li>1. glucose + ATP --Hx-> G-6-P + ADP</li>
    13 KB (2,088 words) - 03:05, 29 September 2011
  • Team:UNIPV-Pavia/Test
    <td class="row"> 0,6</td> <td class="row">1105 [6]</td>
    35 KB (4,868 words) - 19:49, 21 September 2011
  • Team:Gaston Day School/Notebook
    |6 | style="background:#228B22; color:white" | '''''[[6 (June 6, 2011 GDS)|6]]'''''
    6 KB (646 words) - 23:51, 27 September 2011
  • Team:Edinburgh/Biorefinery Appendices
    |Reactor 6 |6,650.82
    10 KB (1,230 words) - 21:58, 10 October 2011
  • Team:UT-Tokyo/LabNote
    *Making TB(pH=6.7), LB plate **EScut : <nowiki>#</nowiki><html><span class="part_num">6</span></html>
    186 KB (30,010 words) - 20:25, 5 October 2011
  • Team:UNIPV-Pavia/Project/Results
    <td class="row"> 0,6</td> <td class="row">1105 [6]</td>
    37 KB (5,126 words) - 12:50, 22 October 2011
  • Team:UCL London/Bibliography
    ...id Psc101 Is a Specific Binding-Site for DNA Gyrase. Embo Journal, 1988. 7(6): p. 1889-1895.</li> <li><a name="magneto-6"></a>Oram, M., et al., A biochemical analysis of the interaction of DNA gyr
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  • Team:Utah State/Notebook
    6. Take competent cells out of the -80 °C freezer, and thaw them on ice ...ol stock and DNA prep), you can place the tip/toothpick into a tube with 5-6 mL LB. There should be enough cells still on the tip/toothpick to have a de
    24 KB (3,974 words) - 21:52, 28 September 2011
  • Team:BYU Provo/Team Thermosensor/Week11
    ===6/27/11=== ===6/28/11===
    2 KB (269 words) - 08:26, 24 September 2011
  • Team:BYU Provo/Team Thermosensor/Week24
    ...,''' 8-21E, '''5-13A,''' 5-13B, '''5-13C,''' 6-3A, 6-3B, '''6-3C,''' 6-3D, 6-3E *Ladder, 6-3F, 6-3G, '''6-3H,''' 4-13A, 4-13B, 4-13C, 4-13D, 4-13E, 4-13F, 4-13G, 4-13H, 5-1B
    2 KB (338 words) - 02:34, 29 September 2011
  • Team:Grinnell CN/Notebook
    ...g <i>esp</i> ligated with <i>rsaA</i> PCR product from 20110605. Only lane 6 shows successful ligation of <i>esp</i> and <i>rsaA</i>.</li> ===Week 6 July 3-9===
    24 KB (3,797 words) - 04:14, 27 September 2011
  • Team:KIT-Kyoto/matsunami/8月
    <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr> <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr>
    39 KB (5,673 words) - 12:26, 4 October 2011
  • Team:Peking S/lab/notebook/cyw
    <font size=6> <font color="#FFFFFF">Yiwei Chen's Notebook</font></FONT> |style="text-align:center"| 6
    15 KB (2,060 words) - 05:11, 5 October 2011
  • Team:OUC-China/Result/week10
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> <p>Since there was only one of the three tubes of 6&leuB turned turbid, we took some bacteria from it for reservation and exami
    6 KB (884 words) - 02:38, 29 October 2011
  • Team:SouthBend-Mishawaka-HS-2/Attributions
    ....edu/~ivy/_Assets/ivy_tech_logo.jpg https://static.igem.org/mediawiki/2010/6/6f/Agilent-2c_Corp_Sig_50mm.jpg https://static.igem.org/mediawiki/2010/6/6c/EMDtwitterlogo.jpg
    9 KB (1,277 words) - 17:09, 24 June 2011
  • Team:Michigan/WikiNotebook
    ⇒Calendar doesn't fit in content section on notebook page. Fixed 6/22/11. Edited width in calendar HTML code and centered via HTML. ⇒IE 6/7/8/9
    1 KB (174 words) - 04:10, 22 June 2011
  • Week 3: May 30-June 3
    To get a 6:1 ratio of insert DNA to vector DNA, the following changes were made to the #6:1 ratio colonies
    6 KB (865 words) - 21:45, 7 June 2011
  • RecA: Week 4, June 6-11
    ==Monday, June 6== ...le again, but the sensor group had similar problems. The RecAI digest from 6/7/11 was ligated with the K3 digest created by the sensor group. The ligati
    7 KB (975 words) - 03:05, 29 September 2011
  • RecA: Week 7, June 26-July 1
    ...ied, then digested with SpeI and XbaI. The K3 vector plasmid (extracted on 6/24) was digested with SpeI and XbaI as well. The digests were ligated toget ...minator (B0015) twice without the cI reporter (I763007). The miniprep from 6/20 and from the registry were amplified through PCR. The terminator (B0015)
    3 KB (508 words) - 18:42, 14 July 2011
  • Team:Bilkent UNAM Turkey/Notebook/
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?116";</styl ...tebook/Week0" title="Team:Bilkent_UNAM_Turkey/Notebook/Week0">Week 0 (6/16-6/22)</a>
    10 KB (1,324 words) - 05:27, 8 July 2011
  • Team:Bilkent UNAM Turkey/Notebook
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?116";</styl ...tebook/Week0" title="Team:Bilkent_UNAM_Turkey/Notebook/Week0">Week 0 (6/16-6/22)</a>
    9 KB (1,286 words) - 16:53, 8 July 2011
  • RecA: Week 7, June 26-July 2
    ...ied, then digested with SpeI and XbaI. The K3 vector plasmid (extracted on 6/24) was digested with SpeI and XbaI as well. The digests were ligated toget ...minator (B0015) twice without the cI reporter (I763007). The miniprep from 6/20 and from the registry were amplified through PCR. The terminator (B0015)
    3 KB (517 words) - 03:02, 29 September 2011
  • Team:UNIPV-Pavia/Calendar/July/settimana3
    <td class="row">6</td> <td class="row">5.6</td>
    25 KB (4,067 words) - 14:29, 17 August 2011
  • Team:Freiburg/Notebook/12 August
    *Incubation at 37°C for 6 hours ...p:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| PR6-6
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  • Team:Freiburg/Notebook/29 August
    ...p:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| GST-6-P-1 a,b,c,d,ePrimer ...der-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| pGEX-6-p-1
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  • Team:Harvard/Template:NotebookDataJuly2
    *RPOZ PCR-Machine 6 |6.63
    33 KB (5,222 words) - 18:48, 3 August 2011
  • Team:Imperial College London/Project/Chemotaxis/Results
    ... start to detect is 10<sup>-8</sup>mole/L. The saturation level is 10<sup>-6</sup>mole/L in which concentration or higher the bacteria’s movements to <p> <b>Figure 6: The dependency of Bias on the concentration of CheY-p</b></p>
    39 KB (5,767 words) - 09:46, 8 September 2011
  • Team:Tec-Monterrey/projectresults
    ...IF (15)SF. (b) Rosetta Gami TI (1)IF (2)SF - TNI (3)IF (4) SF - WT (5)IF (6)SF. BW27783 TI (7)IF (8)SF - TNI (9)IF (10) SF - WT (11)IF (12)SF.(13) PMW ...n all lanes and 1.25 kb of restriction fragment was visualized in the lane 6. (Figure 4)
    24 KB (3,144 words) - 21:16, 20 October 2011
  • Team:Cambridge/Experiments/Synthetic Reflectin PCR and Construction of GA1 to 6
    ...nts/Plasmid_Constructs | Eight plasmids]] were then constructed, named GA1-6, GA13 and GA14 as shown below. Each puts reflectin expression under the con In the case of low copy number plasmids, '''GA2''' and '''GA4-6''', the respective reflectin coding region was joined to a part of pSB1A2 u
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  • Team:Osaka/Notebook
    <h4>Week 6</h4> |[[Team:Osaka/week1#August 6 (Sat)|6]]<span style={{{s6|"color:{{{c6|deepskyblue}}};"}}}></span>
    12 KB (1,674 words) - 10:32, 28 October 2011
  • Team:Queens Canada/Safety/Bioterrorism
    ...cosal surfaces tend to evoke weak immune responses due to poor absorption [6]. To increase absorption, the vaccine could be loaded into a bacterial chas ... therefore symptoms of these agents may be attributed to other conditions [6]. If this happens, a significant number of cases need to accumulate before
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  • Team:UNIPV-Pavia/Calendar/July/week3
    <td class="row">6</td> <td class="row">5.6</td>
    25 KB (4,110 words) - 15:48, 20 September 2011
  • Team:UTP-Panama/Week 9
    ==WEEK 9: AUGUST 1 to 6== 6. All solutions were kept on ice.
    5 KB (688 words) - 02:42, 29 September 2011
  • Team:ETH Zurich/Modeling/Parameters
    |[[Team:ETH_Zurich/Modeling/References#Ref5|[6]]] |[[Team:ETH_Zurich/Modeling/References#Ref7|[6]]]
    9 KB (1,219 words) - 13:36, 28 October 2011
  • Team:Imperial College London/Project/Chemotaxis
    <a href="#Testing">6 Testing</a><br /> ... start to detect is 10<sup>-8</sup>mole/L. The saturation level is 10<sup>-6</sup>mole/L in which concentration or higher the bacteria’s movements to
    44 KB (6,705 words) - 21:17, 14 September 2011
  • Team:Imperial College London/Project Auxin Modelling
    ...t in seed coat to maximally increase root growth of roots is 4.97 x 10<sup>6</sup>. </p> <p>2. The number of moles of IAA need to be in one seed coat is 3.6 * 10<sup>-9</sup> m<sup>3</sup> * 0.1 nmol.
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  • Team:BYU Provo/Team Thermosensor/Week8
    * 6/7 ** Added 6 ul loading dye to each PCR reaction tube and ran on gel @ 160 V for 17 min
    4 KB (599 words) - 08:10, 24 September 2011
  • Team:USC/Notebook/Week12
    [[Team:USC/Notebook/Week6|Week 6]] [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
    6 KB (812 words) - 03:58, 29 September 2011
  • Team:UANL Mty-Mexico/Team/Members
    <b>Sleep hours:</b> 6-9 h<br/> <b>Sleep hours:</b> 6 h<br/>
    31 KB (4,565 words) - 17:34, 13 February 2012
  • Team:Arizona State/Project/B halodurans
    ...called repeat associated mysterious proteins (RAMPs) in the form of CMRs 1-6. ...l. We chose B. halodurans because of its availability and because its CMR1-6 proteins are localized together on its genome, similar to the successful RN
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  • Team:ETH Zurich/Team/Human practices
    : □ 1 □ 2 □ 3 □ 4 □ 5 □ 6 □ 7 □ 8 □ 9 □ 10 : □ 1 □ 2 □ 3 □ 4 □ 5 □ 6 □ 7 □ 8 □ 9 □ 10
    12 KB (1,936 words) - 23:38, 28 October 2011
  • Team:SouthBend-Mishawaka-HS-2/Notebook
    <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD> <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD>
    17 KB (2,546 words) - 17:16, 24 June 2011
  • Team:BU Wellesley Software/Notebook/HeidiNotebook
    '''Week of 6/13''' '''Week of 6/13'''
    8 KB (1,143 words) - 16:36, 27 September 2011
  • Frank Machin's Lab Notebook
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</styl ...User", "3": "User talk", "4": "2011.igem.org", "5": "2011.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10":
    25 KB (3,664 words) - 21:23, 3 July 2011
  • Team:Caltech/Notebook
    <p>[[Team:Caltech/Week_1|Week 1]]: 6/15-6/17: Lab orientation</p> <p>[[Team:Caltech/Week_2|Week 2]]: 6/19-6/25: Lab training; sample collection, enrichment cultures, DNA extraction fr
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  • Team:METU-Ankara
    ... <li> Add 250 uL Lysis Solution and mix gently by inverting the tube 4-6 times until the solution becomes slightly clearPlate aliquots of transform ...ation solution and mix immediately and thoroughly by inverting the tube 4-6 times.</li>
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  • Atipat Patharagulpong's Lab Notebook
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</styl ...User", "3": "User talk", "4": "2011.igem.org", "5": "2011.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10":
    26 KB (3,696 words) - 20:45, 3 July 2011
  • Christopher Schoene's Lab Notebook
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</styl ...User", "3": "User talk", "4": "2011.igem.org", "5": "2011.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10":
    24 KB (3,555 words) - 10:05, 4 July 2011
  • Nicolas Kral's Lab Notebook
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</styl ...User", "3": "User talk", "4": "2011.igem.org", "5": "2011.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10":
    25 KB (3,696 words) - 10:08, 4 July 2011
  • Team:Imperial College London/Extras/Brainstorming
    [6] <a href="http://onlinelibrary.wiley.com/doi/10.1002/iroh.201111355/pdf">ht Spain 1.6<br>
    67 KB (10,740 words) - 10:07, 22 July 2011
  • Team:Arizona State/Project/CRISPR
    ... Cmr (type III), Cst (type I), and Csa (type I) families are present. Cmr1-6 have been found to form a Cascade-like complex that targets RNA in in-vitro <p>''B. halodurans'' contains 6 Cmr genes (Cmr1-6) in a single locus. This is a type III CRISPR system. The organism also con
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  • Team:VIT Vellore/Safety/images
    $(items[currNo]).fadeIn((timeOut/6), function() { $(itemsSpan[currNo]).slideUp((timeOut/6), function() {
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  • Team:VIT Vellore/Safety/images2
    $(items[currNo]).fadeIn((timeOut/6), function() { $(itemsSpan[currNo]).slideUp((timeOut/6), function() {
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  • Team:VIT Vellore/Safety/hoksok
    $(items[currNo]).fadeIn((timeOut/6), function() { $(itemsSpan[currNo]).slideUp((timeOut/6), function() {
    7 KB (623 words) - 18:13, 14 July 2011
  • Team:UC Davis/Notebook/Week 2
    <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_6">6</a></td> --Monday 6/20/11--
    6 KB (875 words) - 07:21, 28 September 2011
  • Team:METU Turkey SoftLab
    ... <li> Add 250 uL Lysis Solution and mix gently by inverting the tube 4-6 times until the solution becomes slightly clearPlate aliquots of transform ...ation solution and mix immediately and thoroughly by inverting the tube 4-6 times.</li>
    134 KB (20,159 words) - 17:29, 13 October 2011
  • Regions/Americas/Pre-Jamboree
    * [[#6|Submit your Team Project Description]] <span id="6"></span>
    22 KB (3,075 words) - 01:15, 29 September 2011
  • Team:Harvard/Protocols
    ...your master solution is in excess of the amount you need (e.g. if you have 6 reactions, make a master solution with 7 or 8 times the volume for each rea [http://www.nature.com/nprot/journal/v1/n1/full/nprot.2006.6.html 2006, Meng, Nature Protocol]
    24 KB (4,085 words) - 03:27, 29 October 2011
  • Team:Arizona State/Templates/cas table
    ...88}} || {{:Team:Arizona State/Templates/tigr|01876}} || y || y || rowspan="6"| N-terminal domain {{:Team:Arizona State/Templates/ref|15}}, crRNA process ...1517}}, {{:Team:Arizona State/Templates/cog|4006}} || || || || rowspan="6"| Helix-turn-helix, transcription regulation {{:Team:Arizona State/Template
    12 KB (995 words) - 01:59, 29 September 2011
  • Team:UNIPV-Pavia/Calendar/August/week3
    Inoculum of E41N-1 and J101-4C5 for plasmid purification 6 ml of LB + Cm12.5. ...in a final volume and 1 ml of 12 ml M9 + Cm12.5 respectively and grown for 6 hours.
    10 KB (1,625 words) - 15:52, 20 September 2011
  • Team:WITS-CSIR SA/Project/Concept
    ...creen" rel="Stylesheet" href="https://ajax.googleapis.com/ajax/libs/dojo/1.6.1/dojo/resources/dojo.css" /> ...creen" rel="Stylesheet" href="https://ajax.googleapis.com/ajax/libs/dojo/1.6.1/dijit/themes/claro/claro.css" />
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  • Team:UTP-Panama/Week 10
    6. Was allowed to stand for 30 minutes on ice. 6. All solutions were kept on ice.
    10 KB (1,542 words) - 04:00, 29 September 2011
  • Team:UNIPV-Pavia/Calendar/August/week4
    ... and E18 cultures were induced with final concentrations of 3OC<sub><small>6</small></sub>-HSL of: 0 nM, 0.1 nM, 0.5 nM, 1 nM, 2 nM, 5 nM, 10 nM and 100 <td class="row">80.6</td>
    18 KB (3,015 words) - 10:12, 18 September 2011
  • Team:ETH Zurich/Modeling/SingleCell
    ...ased on the model published in [[Team:ETH_Zurich/Modeling/References#Ref6|[6]]]. === Initial Model Based on [[Team:ETH_Zurich/Modeling/References#Ref6|[6]]] ===
    17 KB (2,769 words) - 01:06, 29 October 2011
  • Team:UNIPV-Pavia/Tecan Measurements
    <li><b>03-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 0 h and 22 h <li><b>05-09-2011</b> test on M9 and ENTERO4C5 cultures at pH 5.9, 6.3, 6.7, 6.9 (nominal), 7.2 and LB at 1 h and 4 h
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  • Team:TU Munich/lab/notebook/part2
    <p>-part "T7" (date: 17.6., concentration = 190 ng/µl) was digested using X and P. At first we thoug <p>- part "RBS minip" (date: 22.6., concentration = 47.5 ng/µl) was digested using S and P.</p>
    29 KB (4,765 words) - 23:29, 21 September 2011
  • Team:WHU-China/Notebook/Protocols
    font-family:"Times New Roman","serif"'>6.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n font-family:"Times New Roman","serif"'>6.<span style='font:7.0pt "Times New Roman"'>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&n
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  • Team:TU Munich/model/bluelightdata
    ...sons as stated above.</div><h2 class='section'><span class='section_label'>6</span> <a id='magicparlabel-39487' ></a>Conclusion</h2><div class='standard
    48 KB (7,328 words) - 11:37, 28 October 2011
  • Team:UNIPV-Pavia/Calendar/September/week3
    ...es (4ml M9 + Cm12.5 pH = 6.0). After two hours they were induced with aTc (6 ng/ml, 8 ng/ml and 100 ng/ml). Supernatants were taken at the induction tim ...ptember.2C_12th">September, 12th</a> were tested, measuring 3OC<sub><small>6</small></sub>-HSL concentration with T9002-ENTERO in two differents tests.
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  • Team:Potsdam Bioware/Meetings
    ** swimming pool: 4 (6) .... Labjournal 3. Results –Summary page, -BioBricks 4. Modelling 5. Ethics 6. Software 7. Sponsors
    74 KB (9,960 words) - 01:06, 22 September 2011
  • Team:Imperial College London/Project Chemotaxis Modelling
    ...ence the probability of tumbling decreases. From equation 10 in reference [6], we know that when C<sub>t1</sub>-C<sub>t2</sub> >0, the probability of t ...ifferent concentrations. A simulation of chemotaxis of 100 bacteria placed 6 cm away from a 5 mM malate source is shown in the movie below. And this MAT
    22 KB (3,434 words) - 03:57, 29 October 2011
  • Team:Imperial College London/Project Auxin Testing
    <p><i>Figure 6: Salkowski assay performed on IAA producing </i>E. coli<i> and control </i> ...on with the standard Salkowski curve. Surprisingly, the results <b>(Figure 6)</b> suggest that IAA production was optimal in LB, although the OD at 600
    57 KB (9,190 words) - 03:19, 29 October 2011
  • Team:Bielefeld-Germany/Results/NAD
    |rowspan="6"|Purification |36.6
    22 KB (3,374 words) - 00:31, 29 October 2011
  • Team:DTU-Denmark/Background sRNA
    ...coded sRNAs which often only posses a complementary base-pairing region of 6-20 nucleotides and often require Hfq<span class="superscript">[[#References ...thus base-pair with the mRNA. The region of base-pairing is typically only 6-20 nucleotides, and often it is only a subset of these nucleotides which se
    11 KB (1,648 words) - 04:44, 22 September 2011
  • Team:Johns Hopkins/Notebook/YVL
    =====7/6/11===== =====9/6/11=====
    9 KB (1,001 words) - 23:51, 28 October 2011
  • Team:Brown-Stanford/Lab/Notebook/Week13
    *Liquid cultured CHK1.1,3 CHK2.1-6, GPFN == ''' September 6, 2011''' ==
    4 KB (651 words) - 18:11, 28 September 2011
  • Team:Columbia-Cooper/CUNotebook
    <p>Add 10 uL ampicilin to bacteria 6) transfer 25 uL to plate 1</p> <h2>September 6</h2>
    22 KB (3,881 words) - 13:59, 28 October 2011
  • Team:Bielefeld-Germany/Results/S-Layer/SgsE
    ...e right) exhibiting a well-defined periodicity and distances of 9.4 – 11.6&nbsp;nm between the proteinaceous subunits. The S-layer protein SgsE of ''G ...' gene, has a calculated mass of 93.7&nbsp;kDa and an isoelectric point of 6.1. When isolated, SgsE maintains its ability to self-assemble, and in depen
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  • Team:ZJU-China/October.html
    <script src="http://cdn.jquerytools.org/1.2.6/full/jquery.tools.min.js"></script> <td >•Cultivate:Vgb+2I 1-6<br/>•Ligate:fdhf+20H& 1K3 </td>
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  • Team:Osaka/Notebook/diary
    |[[Team:Osaka/week1#August 6 (Sat)|6]]<span style={{{s6|"color:{{{c6|deepskyblue}}};"}}}></span> |[[Team:Osaka/week6#September 6 (Tue)|6]]<span style={{{s6|"color:{{{c6|inherit}}};"}}}></span>
    10 KB (1,315 words) - 04:07, 5 October 2011
  • Team:OUC-China/Result/week4
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> 2. Later at 17:00, extract plasmid from No.5, 4, 6, 2 part.
    6 KB (859 words) - 02:36, 29 October 2011
  • Team:OUC-China/Result/week9
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> <h2>9-6</h2>
    7 KB (1,151 words) - 02:37, 29 October 2011
  • Team:Peking S/project/nonb/comparator
    <font color="#ffffff"> <font size=6>Non-Boolean Population</font></font> ...lly complementary to the translation initiation region of ptsG mRNA, and a 6 nt region overlapping the Shine-Dalgarno sequence of the target mRNA turns
    13 KB (2,029 words) - 01:34, 6 October 2011
  • Team:Grenoble/Projet/Results/Device
    ...6.png"><img src="https://static.igem.org/mediawiki/2011/5/55/Hysteresis_10-6.png" class="centerwide"/></a> ...s="legend"><strong>Figure 3:</strong> Hysteresis curve for $[aTc] = 1x10^{-6} M$</div>
    23 KB (2,980 words) - 01:52, 29 October 2011
  • Team:Copenhagen/Protocol
    6. Discard supernatant and gently resuspend on 5mL cold 6. Leave the plates at 37°C O/N
    20 KB (3,204 words) - 12:16, 26 August 2011
  • Team:Lethbridge/Notebook/Lab Work/Group2
    ==Week 6 June 6 - 12==
    13 KB (1,994 words) - 02:57, 29 September 2011
  • Team:Freiburg/Notebook
    | bgcolor="#FFFFFF" | [[Team:Freiburg/6_April|6]] | bgcolor="#FFFFFF" | [[Team:Freiburg/Notebook/6_May|6]]
    13 KB (1,543 words) - 02:08, 22 September 2011
  • Team:Yale/Notebook/Week4
    <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li> <li>Divided 1 L into 6 separate 100 mL solutions, half with IPTG, induced for 5 hours</li>
    7 KB (1,188 words) - 22:49, 27 September 2011
  • Team:UC Davis/Notebook/Week 1
    <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_6">6</a></td> --Monday 6/13/11--
    9 KB (1,532 words) - 07:40, 28 September 2011
  • Sensor: Week 3 May 30-June 4
    ===cI Repressor Test Assembly, Day 6=== The sensor group checked the colonies from the assembly performed on 6/1/11. The sensor group continued their attempt to add two RBS in front of t
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  • Team:Michigan/DryLabNotebook
    ==6/15/2011== ==7/6/11==
    454 B (66 words) - 21:53, 6 July 2011
  • Team:UANL Mty-Mexico/Team
    '''Sleep hours:''' 6-9 h '''Sleep hours:''' 6 h
    5 KB (847 words) - 21:06, 13 August 2011
  • Team:Freiburg/Notebook/30 August
    ... mM dCTP300 μl of 1 M DTT 1.5 g PEG-8000300 μl of 100 mM NADAdd water to 6 ml Aliquot 100 μl and store at -20 °C ...er mixture. The DNA should be in equimolar amounts. Use 10-100 ng of each ~6 kb DNA fragment. For larger DNA segments, increasingly proportionate amount
    25 KB (2,824 words) - 01:13, 22 September 2011
  • Team:DTU-Denmark-2/Notebook/Lab notes
    <font color=" #990000" face="arial" size="6"> ...011.igem.org/Team:DTU-Denmark-2/Notebook/Lab_notes#Week 6"class="h2"> Week 6</a><br>
    47 KB (7,731 words) - 16:49, 21 September 2011
  • Team:KAIST-Korea/Projects/report 12
    | k<sub>6</sub> | 6*10<sup>-3</sup>/min
    16 KB (2,540 words) - 10:47, 11 August 2011
  • Team:KAIST-Korea/Projects/Modeling/Section1
    | k<sub>6</sub> | 6*10<sup>-3</sup>/min
    18 KB (2,705 words) - 16:17, 12 August 2011
  • Team:Wisconsin-Madison/protocols
    </li><li> Add 400 μl Alkaline Lysis Sol II. Invert 4-6 times to mix. Do not allow reaction to lyse for more than 5 min. Sample s </li><li> Add 300 μl Alkaline Lysis Sol III. Invert 4-6 times to mix. Sample should have a white precipitate.
    27 KB (4,151 words) - 23:09, 28 September 2011
  • Team:Freiburg/Notebook/9 September
    ...07in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| 6 ...0;border-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| 6
    32 KB (3,770 words) - 00:24, 22 September 2011
  • Team:EPF-Lausanne/Notebook/September2011
    == Tuesday, 6 September 2011 == We also went after the remaining non-randomer variants that were left (6, 13, and 14) using a colony PCR on six colonies of each variant. The PCR us
    15 KB (2,527 words) - 08:43, 21 September 2011
  • Team:UNIPV-Pavia/Calendar/September/week1
    ...2, E27-2 and RBS32 in M9 + Amp to collect supernatant after 3OC<small><sub>6</sub></small>-HSL supplementation. ...um in M9 + Cm12.5 of T9002-ENTERO and ENTERO-RBS to measure 3OC<small><sub>6</sub></small>-HSL concentration in supernatants at different pHs collected
    5 KB (851 words) - 10:16, 18 September 2011
  • Team:UNIPV-Pavia/Project/Modelling
    ...class="toclevel-1"><a href="#Sensitivity_Analysis"><span class="tocnumber">6</span> <span class="toctext">Sensitivity Analysis of the steady state of en ...el-2"><a href="#Steady state of enzyme expression"><span class="tocnumber">6.1</span> <span class="toctext">Steady state of enzyme expression</span></a>
    48 KB (7,386 words) - 00:32, 22 September 2011
  • Team:Bielefeld-Germany/Protocols/Analytics
    ** 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm) ** 20 sec orbital shaking (1 mm amplitude with a frequency of 87.6 rpm)
    32 KB (4,735 words) - 02:05, 29 October 2011
  • Team:Potsdam Bioware/BioBricks
    | BBa_K627005 || mdnE || 6 [[File:UP_test of different enzymes.png|center|400px|thumb|'''Figure 6: Phage display against different proteases.''' After panning the number of
    59 KB (9,011 words) - 23:32, 28 October 2011
  • Team:Imperial College London/Project Chemotaxis Testing
    ... thaliana</i> to replicate the experiment by Paungfoo-Lonhienne et al<sup>[6]</sup>. <i>Arabidopsis </i> is a common plant model organism. Its genome ha .... The bacteria were grown to mid-exponential phase (OD<sub>600</sub> 0.4-0.6) before being induced in motility buffer 2 hours prior to observation. Bact
    26 KB (4,242 words) - 03:45, 29 October 2011
  • Team:KIT-Kyoto/matsunami/9月
    <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr> <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr>
    25 KB (3,285 words) - 09:17, 4 October 2011
  • Team:EPF-Lausanne/Our Project/TetR mutants/MITOMI data
    | 6||1.46463||0.164072||1.74384||1.54845 | 6||1.46463||0.164072||1.74384||1.54845
    16 KB (1,939 words) - 21:49, 26 October 2011
  • Team:BYU Provo/Team Thermosensor/Week16
    ==Week 16 (July 30 - Aug. 6)== ===6 August 2011===
    788 B (113 words) - 08:46, 24 September 2011
  • Team:Yale/Parts
    ...creen for ones containing our insert. Lane 1: 100bp ladder, Lanes 3, 5 and 6 show amplification of our insert; these colonies were subsequently miniprep ...e 4: RBS-RiAFP-His-Terminator, Lane 5: RBS-His-eGFP-TmAFP-Terminator, Lane 6: RBS-TmAFP-His-Terminator, Lane 7: RBS-His-eGFP-TEV-TmAFP-Terminator, Lane
    6 KB (790 words) - 15:20, 28 October 2011
  • Team:Brown-Stanford/Lab/Notebook/Week3
    **Grew 6 liquid cultures from liquid stock (100 mL) **Grew 6 liquid cultures from glycerol stock
    8 KB (1,234 words) - 18:09, 28 September 2011
  • Team:Northwestern/Project/Background
    ...'', namely, the Las and the Rhl signaling pathways<html><sup><A HREF="#6">[6]</A></sup></html>. ...roduction of the PAI-2’s cognate R-protein, RhlR<html><sup><A HREF="#6">[6]</A></sup></html>. Contrary to the las system, RhlR can be deactivated by t
    11 KB (1,643 words) - 02:40, 29 October 2011
  • Team:Calgary/Project/Promoter/Bioinformatics
    ...ml>[[Image:UofC2011_ECHdissociation_curve.jpg|thumb|300px|center|<b>Figure 6</b> RT-PCR melt curve of ECH primer set with LD2 cDNA ]]<html> ...ed with different cDNA levels of the strain LD2, as demonstrated in figure 6. The ECH primers showed a strong dissociation curve with a single peak sugg
    28 KB (4,301 words) - 04:02, 29 October 2011
  • Team:Calgary/Project/Chassis/Pseudomonas
    ...at 37&deg; before plating. We tried incubating after 10 minutes, 2 hours, 6 hours and 12 hours. <td style="padding-left: 10px; padding-right: 10px;"><p>6 hrs</p></td>
    16 KB (2,722 words) - 03:58, 29 October 2011
  • Team:UNAM-Genomics Mexico/Notebook/repC
    ...plated and extracted the plasmid of five repC bacterial colonies (1, 2, 5, 6, 9).We performed more ligation reactions with different insert:vector ratio 2-6 Digestions with SpeI of plasmid extractions of strains 1, 2, 5, 6, 9.
    8 KB (1,256 words) - 19:48, 28 September 2011
  • Team:KIT-Kyoto/DIAP2-MALT実験９月
    <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr> <tr><td align=center>6 x loading dye</td><td align=right>2 µl</td></tr>
    25 KB (3,288 words) - 09:24, 4 October 2011
  • Team:Tokyo-NoKoGen/protocols
    <meta name="GENERATOR" content="Freeway 5 Express 5.6.1"> <td height=6><img src="Resources/_clear.gif" border=0 width=1 height=1 alt="" style="flo
    17 KB (2,608 words) - 11:19, 5 October 2011
  • Team:Tokyo Tech/Modeling/Urea-cooler/figures
    ...ttp://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,0,0" width="965" height="150" id="Yourfilename" align=""> <a href="#Elem1">Elementary Flux Modes (Fig. 6)</a>
    12 KB (1,695 words) - 12:55, 28 October 2011
  • Team:OUC-China/Result/week5
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> ... shook 14-19. We also tried to pick a few colonies to shake and culture. 2-6, which was reserved earlier, was together shaken and cultured. We await ext
    5 KB (786 words) - 02:36, 29 October 2011
  • Team:Peking S/project/wire/harvest
    <font color="#ffffff"> <font size=6>Chemical Wire Toolbox</font></font> ...ure was obtained using enzyme-labeled assay. The result is shown in figure 6.
    12 KB (1,698 words) - 04:02, 6 October 2011
  • Team:Bielefeld-Germany/Results/S-Layer/CspB CG
    ...nt after cultivation, periplasmatic isolation, cell lysis, denaturation in 6 M urea and the following wash with 2 % (v/v) Triton X-100, 2 % SDS (w/v) we |style="border-style: solid; border-width: 0"| 2.6
    22 KB (3,144 words) - 03:46, 29 October 2011
  • Regions/Asia/Jamboree/test page
    <li><a href="#6">Select your track</a></li> <h2 class="jamboree"><a name="6"></a>Tracks </h2>
    19 KB (3,141 words) - 10:16, 28 April 2011
  • Regions/Asia/Jamboree/Pre-Jamboree
    <li><a href="#6">Select your track</a></li> <h2 class="jamboree"><a name="6"></a>Tracks </h2>
    19 KB (3,134 words) - 01:37, 29 April 2011
  • Regions/Asia/Jamboree/Pre-regional Jamboree
    <li><a href="#6">Select your Track</a></li> <h2 class="jamboree"><a name="6"></a>Tracks </h2>
    27 KB (4,381 words) - 12:45, 13 October 2011
  • Team:Wageningen UR/Softwaretool
    ...mple, you want to create the following hypothetical construct of 1-2-3-4-5-6. A number in this construct represents what we call a functional genetic mo The hypothetical construct consists of 6 of these FGM’s. Because each FGM can function on its own due to the promo
    5 KB (884 words) - 03:37, 22 September 2011
  • Team:SouthBend-Mishawaka-HS-2
    case 6: document.getElementById("pic"+num).src =suparray[effect].src; ...6)" onMouseOut="mouseOut(6)" onMouseDown="mouseDown(6)" onMouseUp="mouseUp(6)">
    10 KB (1,356 words) - 17:14, 24 June 2011
  • Team:Nevada/Notebook
    <li>1. glucose + ATP --Hx-> G-6-P + ADP</li> <li>2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH</li>
    3 KB (548 words) - 20:32, 30 June 2011
  • Team:UQ-Australia/Project
    ...cillators from both Atkinson <i>et al</i>. [5] and Stricker <i>et al</i>. [6]. In addition, we plan on using an engineered promoter denoted as Plac/ara, ...d therefore having cell-to-cell differences in the rate of oscillation [4, 6].
    10 KB (1,473 words) - 00:37, 6 October 2011
  • Team:Grinnell/Notebook/Protocols
    <li>0.6&mu;L DMSO</li> <tr><td>6</td><td>3&deg;C above the T<sub>m</sub> of the primer (with prefix/suffix)
    21 KB (3,695 words) - 04:12, 29 September 2011
  • RecA: Week 4, June 6-10
    ...le again, but the sensor group had similar problems. The RecAI digest from 6/7/11 was ligated with the K3 digest created by the sensor group. The ligati ...easured when transforming. This ligation was redone using the digests from 6/7/11, and was transformed and plated to let colonies grow overnight.
    5 KB (794 words) - 17:31, 23 June 2011
  • Team:Nevada/Notebook/June/Week1
    Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
    3 KB (425 words) - 23:13, 28 June 2011
  • Sensor: Week 4 June 6-11
    The minipreps prepared on 6/4 (K648000+K648001, B0030+C0051, B0032+C0051) were sent to sequencing. The ...attempted to insert the Cro and O<sub>R</sub> parts created through PCR on 6/3 into separate C3 vectors through amplified insert assembly.
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  • Team:Nevada/Notebook/June/Week4
    Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
    2 KB (351 words) - 20:07, 27 June 2011
  • Team:Nevada/Notebook/June/Week5
    Rxn: 1. glucose + ATP --Hx-> G-6-P + ADP 2. G-6-P + NAD+ --G-6-PDeH-> G-6-P + NADH
    1 KB (183 words) - 19:12, 23 June 2011
  • Team:Paris Bettencourt/Notebook
    ...ttps://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/06/06/"> 6</a></td> ...ttps://2011.igem.org/Team:Paris_Liliane_Bettencourt/Notebook/2011/07/06/"> 6</a></td>
    19 KB (2,638 words) - 09:33, 21 October 2011
  • Team:UNIPV-Pavia/Calendar/July/settimana4
    <td class="row">6</td> <td class="row">6.4</td>
    20 KB (3,189 words) - 14:35, 17 August 2011
  • Team:ETH Zurich/Biology/Validation
    ...s already observed, as can be seen by a decreased OD<sub>600</sub> (Figure 6). The promoter was found to be rather strong, as it showed expression rates File:Influence on cell growth.png|'''Figure 6: Influence of xylene on the cell growth of E.coli.''' Xylene shows toxic ef
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  • Team:CongoDRC-Bel Campus/Team
    var escapeRegExp = function(string){// Credit: XRegExp 0.6.1 (c) 2007-2008 Steven Levithan <http://stevenlevithan.com/regex/xregexp/> // Opera 9.6 getElementsByClassName doesnt detects the class if its not the first one
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  • Team:Fudan/Lab
    arr[6]="https://static.igem.org/mediawiki/2011/3/39/Fudan_lab_06.jpg"; arr[9]="https://static.igem.org/mediawiki/2011/6/66/Fudan_lab_03.jpg";
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  • Team:Fudan-Shanghai/Lab
    arr[6]="https://static.igem.org/mediawiki/2011/3/39/Fudan_lab_06.jpg"; arr[9]="https://static.igem.org/mediawiki/2011/6/66/Fudan_lab_03.jpg";
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  • Sunday, August 21
    Jessica Hobson 2 p.m.-6:30 p.m. Dr Liz ''' I'll be available for phone consulting from 2:00 - 6:00+ @ 443.610.5259. ''' I'm very glad Patrick can open the lab today as I'
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  • Team:Wisconsin-Madison/notebookprotocols
    </li><li> Add 400 μl Alkaline Lysis Sol II. Invert 4-6 times to mix. Do not allow reaction to lyse for more than 5 min. Sample s </li><li> Add 300 μl Alkaline Lysis Sol III. Invert 4-6 times to mix. Sample should have a white precipitate.
    24 KB (3,605 words) - 16:25, 8 August 2011
  • Team:Harvard/Notebook
    if(k==6){ 6
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  • Team:Lyon-INSA-ENS/Realisation/Protocols/DNA-purification
    <b>4.</b> Resuspend the bacterial pellet in 6 mL Buffer P1. ...d 6 mL Buffer P2, mix thoroughly by vigorously inverting the sealed tube 4-6 times, and incubate at room temperature (15-25°C) for 5 min.
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  • Team:WashU/Notebook/July2011
    Master mix composition (6 wells worth) ==July 6==
    18 KB (2,731 words) - 04:45, 28 September 2011
  • Team:Macquarie Australia/Notebook
    * Step 6) We set up the PCR machine with the following protocol: 6. Mix well – centrifuge for a few seconds
    27 KB (4,274 words) - 11:05, 4 October 2011
  • Team:Freiburg/Notebook/22 August
    ...der-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| pgEX-6-p-1a 1:10 ...der-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| pgEX-6-p-1a 1:10
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  • Team:Caltech/Week 8
    <td>6.2</td> Miniprepped colonies from Gibson colony pcr (pNT003-2,8,11,12 and pNT002-6,11,12,14)<br/>
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  • Team:St Andrews/diary
    <h3>Meeting 6</h3> <a href="#week6">Week 6</a>
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  • Team:Harvard/Template:NotebookDataJuly3
    6 84_4 16 83_4 We then ran a PCR for the cross-junction on all 6 (80.1, 80.2, 81.1, 81.4, 85.1, 85.2) samples, using our usual cross junctio
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  • Team:Harvard/Template:NotebookDataJuly4
    ****156.2 ng/µL and 107.6 ng/µL, each in 30ul of water (distilled, deionised- molecular grade) **Time constant of 5.6 ms
    29 KB (4,742 words) - 19:22, 3 August 2011
  • Team:UNIPV-Pavia/Calendar/July/week4
    <td class="row">6</td> <td class="row">6.4</td>
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  • Team:Peking S/lab/notebook/sr
    |style="text-align:center"| 6 Gel 3 Well 1-6: Marker_lacI_lacI_ _ _lacI_lacI
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  • Team:UTP-Panama/Week 14
    ==September 6== #6 tubes
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  • Team:UTP-Panama/Week 15
    Cultivation 9/6/2011 2. In each eppendorf 1.5mL of cultivation (9/6/2011) control (+)<br>
    3 KB (454 words) - 03:50, 29 September 2011
  • Team:Nevada/Project
    <img src="https://static.igem.org/mediawiki/2011/6/69/Lauren2.JPG" width="800"> <b>Figure 6. Agarose gel electrophoresis of PCR amplified Zymonomas mobilis glucose fac
    40 KB (6,387 words) - 04:02, 29 September 2011
  • Team:UNIPV-Pavia/Calendar/August/week5
    ...n <a name="August.2C_27th">August, 27th</a> and 10 &mu;l of 3OC<sub><small>6</small></sub>-HSL. ...ycerol 80 %, 8 ml M9 salts 5 x and their pH was adjusted to 5.9, 6.3, 6.7, 6.9 and 7.2; they were autoclaved in order to prepare M9 glycerol supplemente
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  • Team:Freiburg/Notebook/5 September
    ...07in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| 6 ...07in solid #000000;border-right:0.0007in solid #000000;padding:0.0382in;"| 6
    27 KB (2,935 words) - 00:26, 22 September 2011
  • Team:DTU-Denmark-2/Project/PlugnplayAssembly/customization
    <font color=" #990000" face="arial" size="6"> <dd>6. After receiving the primers, you are ready to perform the PCR (see protoco
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  • Team:Caltech/Week 13
    ==September 6== <td>6, one white</td>
    3 KB (474 words) - 18:31, 13 September 2011
  • Team:Bielefeld-Germany/Protocols/Materials
    * 6 M urea * 25 mM Sodiumacetate, pH 6
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  • Team:ETH Zurich/Biology/Detector
    <img src="/wiki/images/6/61/Moduls_of_SmoColi.png" usemap="#Moduls"/> ...h same affinity. In contrast to other members of the Zn<sub>2</sub>Cys<sub>6</sub>, the DNA binding domain is stretch of 16 amino acid residues between
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  • Team:UNIPV-Pavia/Project/References
    ...e expression in bacteria.</b> <i> Appl. Environ. Microbiol.</i> 64(6):2240-6. ... encoding models for synthetic biology. </b> <i>J. R. Soc. Interface. </i> 6:S405-S417.
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  • Team:Imperial College London/Project/Chemotaxis/Modelling
    ... start to detect is 10<sup>-8</sup>mole/L. The saturation level is 10<sup>-6</sup>mole/L in which concentration or higher the bacteria’s movements to ... and hence the probability of tumbling decreases. From equation 10 in ref [6], we known that even if C_t1-C_t2 >0, the probability of tumbling could de
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  • Team:UNIPV-Pavia/Parts/Characterized
    ...s="tocnumber">2.3</span> <span class="toctext">p<sub>Lux</sub> - a 3OC<sub>6</sub>-HSL-in PoPs-out device</span></a></li> <li class="toclevel-2"><a href="#AiiA"><span class="tocnumber">2.6</span> <span class="toctext">AiiA - BBa_C0060</span></a></li>
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  • Team:KIT-Kyoto/Notebook/LabNote/GFPMLF8
    :<tr><td align=center>10 x H Buffer</td><td align=right>6 µl</td></tr> :<tr><td align=center>10 x H Buffer</td><td align=right>6 µl</td></tr>
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  • Team:UC Davis/Notebook/Week 15
    <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_6">6</a></td> |0.6% || 0.0042%
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  • Team:Peking R/Notebook/ZYY
    <th width="54" rowspan="6" bgcolor="#FFFFFF" scope="row"><p>&nbsp;</p> ...:100 with LB medium in 96 deep well plates and cultivate until the OD is 0.6 to add different concentration of TPP according to the following chart. Aft
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  • Team:USC/Notebook/Week6
    [[Team:USC/Notebook/Week6|Week 6]] [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
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  • Team:USC/Notebook/Week10
    [[Team:USC/Notebook/Week6|Week 6]] [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
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  • Team:Peking R/Notebook/ZZR/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,479 words) - 17:29, 25 September 2011
  • Team:Brown-Stanford/Lab/Notebook/Week9
    *Nanodrop readings: GFP: 30; Nos: 6; Ana: 11; however, spectra were suspicious and 260/280 ratios were >2.5 *added digested inserts (6:1 ratio for Ana and GFP, 3:1 ratio for Nos; on account of feasible concentr
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  • Team:UANL Mty-Mexico/Modelling/Biphasic Switch
    <p>cI/0.6</p> .../2011/d/d7/Fig_6_-_Efficiencies_1_Mnt.png" rel="lightbox" title="<b>Figure 6</b> – System behavior at different efficiencies, considering one Mnt gene
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  • Team:Lethbridge/Results
    ...rown in LB media and incubated overnight with shaking at 37°C. Samples #1-6 was grown up differently with specific treatments for the expression of cer ...ntil they reached an OD600 of 0.6 (approximately 3 hours). Samples 1-3 and 6-10 were immediately pelleted by centrifugation (4oC, 5000xg, 20min) and pla
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  • Team:Grinnell CN/Notebook/Protocols
    <li>0.6&mu;L DMSO</li> <tr><td>6</td><td>3&deg;C above the T<sub>m</sub> of the primer (with prefix/suffix)
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  • RecA: July 10 - September 28
    • incubated 4-6 hours </br> o Part 2 T.C.: 2.6 </br>
    11 KB (1,697 words) - 03:03, 29 September 2011
  • Team:Calgary/Notebook/Journals/OLD ENTRIES
    <h4>May 6, 2011</h4> ...labs, they introduced iGEM and microbiology research to students in grades 6, 8, 9, and 12.</p>
    20 KB (3,325 words) - 04:36, 29 September 2011
  • Team:Tsinghua-A/Modeling/P2A
    ...:Tsinghua-A/Modeling/P3A"><img src="https://static.igem.org/mediawiki/2011/6/6e/ThuA_D1.png" width="110px" height="60px"></A></div> ...m:Tsinghua-A/Modeling/P4"><img src="https://static.igem.org/mediawiki/2011/6/6a/ThuA_E1.png" width="110px" height="120px"></A></div>
    20 KB (3,028 words) - 03:52, 28 October 2011
  • Team:Peking S/lab/notebook/wdq
    <font size=6> <font color="#FFFFFF">Dingqiao Wen's Notebook</font></FONT> |style="text-align:center"| 6
    18 KB (2,417 words) - 05:07, 5 October 2011
  • Regions/Americas/DuringTheJamboree
    ...d-iF7rAc3GAuHXw%3BFRXpXgIdhBnd-iGSKM6dJw-Znym_ik-dylBriDHouRf_a2h_9g&vpsrc=6&dirflg=w&mra=ltm&t=m&z=16 Click to view Walking Directions (GoogleMaps)]</s |6:00 PM - 10:00 PM
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  • Regions/Americas/Practice Presentations
    | '''Room 6 &nbsp;&nbsp;''' | '''6:00p - 6:30p'''
    2 KB (194 words) - 14:26, 8 October 2011
  • Team:Peking S/lab/notebook/jwy
    <font size=6> <font color="#FFFFFF">Weiyue Ji's Notebook</font></FONT> |style="text-align:center"| 6
    18 KB (2,417 words) - 05:21, 5 October 2011
  • Team:OUC-China/Result/week8
    <li><a href="/Team:OUC-China/Result/week6">Week 6</a></li> ...s to Lb liquid medium for further use. In the evening we ligated 5&4 and 2-6&3 which digested by enzymes before.</p>
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  • Team:SJTU-BioX-Shanghai/Notebook/Lablog2
    ddH2O 6.2ul ==9/6/2011==
    9 KB (1,367 words) - 15:17, 5 October 2011
  • Team:Grenoble/Projet/Results/Toggle
    ...of $1.10^{−6} M$ to $1.10^{−2} M$ and an aTc concentration of $5.10{−6} M$. The X axis represents physical points on the plate, from the right sid ...rediction of the switch on the plate for an aTc concentration of $5.10^{−6} M$. Upper panel:
    28 KB (3,850 words) - 03:50, 29 October 2011
  • Team:OUC-China/Result/2011
    <td>6</td> <td>5-4-6-leuB-6-2-3</td>
    4 KB (630 words) - 22:01, 5 October 2011
  • World Championship Jamboree/Workshops
    <td rowspan="6" style="width: 200px;" valign="top"> <td rowspan="6">
    4 KB (585 words) - 19:36, 4 November 2011
  • World Championship Jamboree/Schedule/Workshops
    <td rowspan="6" style="width: 200px;" valign="top"> <td rowspan="6">
    3 KB (501 words) - 19:38, 25 October 2011
  • Team:KULeuven/Results
    ...se of fluorescence compared to the control without arabinose for the first 6 hours. The observed increase in fluorescence after arabinose addition at th ... cells/mL) using different kinds of water. Each time we pipetted 10µL (10^6 cells) in the sample cell and in the reference cell we put nothing. Afterwa
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  • Team:Grenoble/Notebook
    <h1>June 29<SUP>th</SUP> to July 6<SUP>th</SUP></h1> ...r 6 out of 10 ligation results. PCR checks were performed as above for the 6 valid ligations. Only 2 appeared to have the right size. The sequencing of
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  • Team:Wageningen UR/Team
    | +31 6 3304 4948 | +31 6 3136 3576
    10 KB (1,278 words) - 23:59, 18 September 2011
  • Team:Cornell/Week 1
    ==Monday, June 6== :::6. Cut out the band of right size, gel purify, and quantify with NanoDrop.
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  • Team:Greenfield IN-Schini-HS/Notebook
    <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD> <a href="https://2011.igem.org/Team:Greenfield_IN-Schini-HS/April 6th, 2011">6</font><br><br><br><br></TD>
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  • Team:Wageningen UR/Project/CompleteProject1Description
    '''Fig.6.''' ''Genetic circuit of the synchronized oscillating system on a single pl '''6''': Constitutively expressed RFP allows the distinction between the effects
    19 KB (2,886 words) - 03:53, 22 September 2011
  • Team:UPO-Sevilla/Foundational Advances/MiniTn7/Experimental Results/Characterization
    <td>6 x 10<sup>9</sup></td> ...arbenicillin plates, clones were patched onto LB-gentamycin and LB plates. 6 out of 100 candidates failed to grow on the LB-gentamycin plates, indicatin
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  • Team:Brown-Stanford/Lab/Notebook
    [[Team:Brown-Stanford/Lab/Notebook/Week0|6]] [[Team:Brown-Stanford/Lab/Notebook/Week4|6]]
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  • Team:Uppsala-Sweden/Notebook
    ccaS and ccaR.<br> Mutagenesis:<br> amilCP and amilGFP.<br> There were 6 clones of each transformants, 42 samples in total. == Week 6 ==
    55 KB (8,809 words) - 15:16, 25 October 2011
  • Team:Northwestern/Notebook
    '''[[Team:Northwestern/Notebook/Week1|Week 1 - 6/12 to 6/18]]''' '''[[Team:Northwestern/Notebook/Week2|Week 2 - 6/19 to 6/25]]'''
    809 B (92 words) - 18:16, 6 July 2011
  • Sensor: Week 5 June 12-17
    &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;The assemblies that were cultured on 6/10/11 (J23113+B0030+C0051, J23113+B0031+C0051, B0032+C0051 were sent to seq ...aced in front of the cI repressor testing construct assembled on Monday, 6/6/11 as well as in front of a terminator and mCherry reporter.
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  • Team:UC Davis/Notebook/Week 12
    <td><a href="https://2011.igem.org/Team:UC_Davis/Notebook/Week_6">6</a></td> The first characterizing run will be for our "good mutants" #1-6 at 0% arabinose. The four levels of IPTG that we have decided to start with
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  • Team:UC Davis/Protocols
    **Invert 4-6 times until the solution become clear. ** Invert 4-6 times.
    12 KB (1,920 words) - 21:10, 23 October 2011
  • Team:Caltech/Week 6
    [[File:Caltech_7_19_Gel_1.jpg|thumb|left|Colonies 1-6 from Gibson plates; 7 and 8 are reswabs of two colonies. From left to right ... 1 NEB 2-log ladder, 2 GFP 0 min, 3 GFP 5 min, 4 GFP 10 min, 5 GFP 15 min, 6 GFP 30 min, 7 GFP 60 min, 8 blank, 9 pNT001 0 min, 10 pNT001 5 min, 11 pNT0
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  • Team:Nevada/Notebook/Weeks58
    <font size=6>Calender Weeks 5-8</font> =='''Week 6 - July 4th-10th'''==
    23 KB (3,836 words) - 03:00, 29 September 2011
  • Team:UNIPV-Pavia/Parts/DataPage
    ...>(p<sub>Lambda</sub>-RBS30-LuxR-T-p<sub>Lux</sub>-RBS34-mRFP-TT) - 3OC<sub>6</sub>-HSL biosensor, mRFP output. </b> <br> ...</td><td class='row'>1.6814</td><td class='row'>2.45</td><td class='row'>0,6</td>
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  • Reporter: Week 8 July 3-July 9
    ===Assembly of Fusion Parts, Day 6=== ==Wednesday, July 6==
    6 KB (882 words) - 00:00, 31 July 2011
  • Team:Edinburgh/tech/colours
    background: url('/wiki/images/6/63/Edinburgh_home_hover_mask.png'); background: url('/wiki/images/6/6b/Edinburgh_sponsors_select_mask.png');
    7 KB (1,029 words) - 18:31, 21 September 2011
  • Sensor: Week 8 July 5-8
    ==Wednesday, July 6== The sensor group verified their assemblies from 7/6 through agarose gel electrophoresis. The C0051+B0033 looked good, but the C
    3 KB (503 words) - 18:27, 13 July 2011
  • Team:EPF-Lausanne/Tools/Microfluidics/HowTo2
    | Luer lock needles .6 mm ID, 12.7 mm length || Dosieren.net || 100 || 0.28 || 28.00 | PE Tubing (6 mm x 4 mm ODxID), 100 m || Serto || 1 || 30 || 30.00
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  • Team:Arizona State/Notebook/July
    == Wednesday, July 6 == : 6. 2x da gingko in psb1k3
    23 KB (3,494 words) - 02:54, 29 September 2011
  • Team:Harvard/Project
    ...rmation coming from a previous zinc finger study called CODA[[#References|[6]]]. ...w for the amplification of specific sub-pools: in our team's case, we used 6 sub-pools, one for each of our target sequences.
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  • Team:Freiburg/Notebook/18 July
    ...-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6''' ...-left:0.0007in solid #000000;border-right:none;padding:0.0382in;"| '''Lane 6'''
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  • Team:Freiburg/Notebook/19 July
    ...g-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 6 ...g-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 6
    30 KB (3,509 words) - 00:58, 22 September 2011
  • Team:Freiburg/Notebook/26 July
    ...;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| Had only 6 tet plates -> put 100 μl of each sample on each plate # heat for 1-2 hours 37°C (6 hours if time)
    24 KB (2,938 words) - 01:01, 22 September 2011
  • RecA: Week 8, July 3-9
    ==Wednesday, July 6== &nbsp;nbsp;nbsp;nbsp;nbsp;The cultures from 7/6 were accidentally placed into the 50&deg;C incubator instead of the 37&deg;
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  • Team:Peking R/Notebook/LZ
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,432 words) - 13:44, 16 September 2011
  • Team:Cornell/Week 10
    :::18.6μL VioE in AviTagged pZE12 vector backbone (1μg) :::5.6μL H2O (gene insert volumes go toward water volume)
    13 KB (1,865 words) - 17:23, 18 September 2011
  • Team:Peking S/lab/notebook/yrf
    <font size=6> <font color="#FFFFFF">Ruofan Yu's Notebook</font></FONT> |style="text-align:center"| [[Team:Peking_S/lab/notebook/yrf#7.6|6]]
    11 KB (1,328 words) - 12:03, 19 August 2011
  • Team:Korea U Seoul/Notebook
    =='''6/24 - Plans for summer break'''== [[File: Lab photo 6.jpg|center|200px]]
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  • Team:Amsterdam/Notebook/Protocols/Digestion
    |align="right"|6 6. take 1 μl of each sample.<br>
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  • Team:UNIPV-Pavia/Calendar/August/week1
    <td class="row">21.6</td> ...png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/6/6f/UNIPV_02_08_2011_E41-AGAIN-1_EP_E41-AGAIN-2_EP_E28-AGAIN-1_EP_E28-AGAIN-
    13 KB (2,104 words) - 10:02, 18 September 2011
  • Team:EPF-Lausanne/Notebook/August2011
    4.2 64ng/µl | 4.3 140ng/µl | 5.1 69ng/µl | 5.3 65ng/µl | 6.2 44ng/µl ...LP52_BP5col6.pdf "PQ39YM42LP52"] [https://static.igem.org/mediawiki/2011/6/6c/PQ39YM42_BP5col4_insertion_deletion.pdf "PQ39YM42_ins_del"]
    39 KB (6,395 words) - 09:04, 21 September 2011
  • Team:DTU-Denmark-2/Team/Protocols
    <font color=" #990000" face="arial" size="6"> ...am:DTU-Denmark-2/Team/Protocols#Coverslips for microscopy" class="h2"><b>5.6</b> Coverslips for microscopy</a><br>
    51 KB (7,868 words) - 09:05, 4 October 2011
  • Team:Grenoble/Notebook/June
    <li>Tests : (6 plasmids)</li> ...r 6 out of 10 ligation results. PCR checks were performed as above for the 6 valid ligations. Only 2 appeared to have the right size. The sequencing of
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  • Team:Paris Liliane Bettencourt/Notebook/2011/08/11/
    The tubes of 5 and 6, that where identified to be the uncorrect sequence this time are supposed Tube 6: 203 ng/µL (ratio 2,1)
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  • Team:Osaka/week1
    ==August 6 (Sat)== ...week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[Te
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  • Team:Paris Liliane Bettencourt/Notebook/2011/08/12/
    ...he other gel of the experience above, we meet the same problems: the weels 6 contains the plasmid from the gel extraction of the lower band and eventhou *2.4 : 8,8 ng - 2.5 : 6,2 ng
    4 KB (654 words) - 16:09, 27 August 2011
  • Team:WHU-China/gu
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?270";</styl ...User", "3": "User talk", "4": "2011.igem.org", "5": "2011.igem.org talk", "6": "File", "7": "File talk", "8": "MediaWiki", "9": "MediaWiki talk", "10":
    22 KB (2,888 words) - 14:19, 14 August 2011
  • Team:Paris Liliane Bettencourt/Notebook/2011/08/17/
    6 µL dNTPs 6 µL Primer fwd at (50µM)
    1 KB (171 words) - 11:52, 18 September 2011
  • Team:UNICAMP-EMSE Brazil/protocols/Mini prep
    ...d 250 μl of the Lysis Solution and mix thoroughly by inverting the tube 4-6 times until the solution becomes viscous and slightly clear. ...zation Solution and mix immediately and thoroughly by inverting the tube 4-6 times.
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  • Team:UNICAMP-EMSE Brazil/Notebook/22 August 2011
    :*Quantification (volume of ladder applied 6 uL; sample: 5 uL DNA +1 uL buffer ) ...''3''' and '''4''' = SoxS promoter - 2ul of the miniprep<br>'''5''' and '''6''' = Constitutive promoter - 2ul of the miniprep<br>'''7''' = flhDC promote
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  • Team:UTP-Panama/Week 2
    ==Week 2: June 6 to 11== '''Work Session #6''' (morning)<br>
    1 KB (190 words) - 18:43, 25 September 2011
  • Team:Paris Liliane Bettencourt/Notebook/2011/09/21/
    * 1:1 : Minsert = 6,30 ng -> 0,63 µL (ligation in 20 µL) * 1:6: Minsert = 38 ng -> 3,8 µL (ligation in 20 µL)
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  • Team:Paris Liliane Bettencourt/Notebook/2011/08/29/
    500ng from the tubes 1 and 6 was digested in XP for 1h and the digestion runned on a gel. [[File:CPGel2908_1.jpg|center|thumb|ladder - 1 - 6]]
    1 KB (238 words) - 15:15, 3 September 2011
  • Team:TU Munich/team/teamoverview
    <li><b>Semester:</b> 6</li> <li><b>Semester:</b> 6</li>
    24 KB (3,328 words) - 02:13, 29 October 2011
  • Team:Paris Liliane Bettencourt/Notebook/2011/08/30/
    6 µL dNTPs 6 µL Primer fwd at (50µM)
    2 KB (272 words) - 15:21, 3 September 2011
  • Team:Tsinghua/experiment
    | style="width: 40px;"| 6 | ''6. S 0.1mM 18℃ ''
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  • Team:Tsinghua/Notebook/4 June 2011
    blank DELL 2011-6-4 17:02:14 0.6 ng/µl final dissolve DELL 2011-6-4 17:02:52 4.4 ng/µl
    377 B (57 words) - 14:40, 2 September 2011
  • MSDS from Promega
    * 6 Accidental release measures (Contd. on page 6)
    13 KB (1,791 words) - 03:52, 3 September 2011
  • Team:Osaka/week6
    ==September 6 (Tue)== ...]]|[[Team:Osaka/week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|'''6'''|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|[[T
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  • Team:Bilkent UNAM Turkey/TEST
    if(k==6){ 6
    14 KB (1,904 words) - 05:45, 4 September 2011
  • Team:Imperial College London/Extras/Protocols
    <p>Transformation of cells with 6, 7 and 8:<br> ...ce the gradient looks exponential (usually around OD<sub>600</sub> 0.4 - 0.6 after multiplying x10 due to dilution), cells are ready to use.<br>
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  • Team:UNIPV-Pavia/Modelling03
    <li class="toclevel-2"><a href="#References"><span class="tocnumber">1.6</span> <span class="toctext">References</span></a></li> ...ng Models for Synthetic Biology", Journal of the Royal Society, 2009 Aug 6;6 Suppl 4:S405-17. Epub 2009 Apr 1.<br><br>
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  • Labjournal may
    ...l 629 (digested) + 6 µl 6x loading dye solution, 30 µl 654 (digested) + 6 µl 6x loading dye<br> 0,5 g Agarose, 50 ml TAE (1%), 2 µl GELRED (3-6µl), at 100 Volt,running <br>
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  • Team:TU Munich/model/andgatedata
    ...1998/Math/MathML'> <mrow> <msub> <mrow><mi>x</mi> </mrow> <mrow><mn>6</mn> </mrow> </msub> </mrow></math></div></td><td align='center' valign=
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  • Team:Brown-Stanford/PowerCell/Background
    ...sis is approximately 11% of solar energy, with a practical efficiency of 3-6%.{{:Team:Brown-Stanford/Templates/FootnoteNumber|A}} Added to the fact tha |6.36 mb pressure
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  • Team:Groningen/project notebook/9 September 2011
    Flowcytometry measurement of construct: PBad_GFP_DT (colony 4 & 6) <br> <br> Measure GFP expression with the FACS for PBAD-RBS-GFP-DT colonies 4 and 6
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  • Team:Lethbridge/Notebook/Lab Work/Justin
    =Week 6 (June 6-12 )= *6) pSB1A3 (J04450)
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  • Team:UNIPV-Pavia/Project/Solution
    ...tR/O and AraC/I1-I2 regulatory elements. </b> <i>Nucleic Acids Res.</i> 25(6):1203-10. ...g signal processing tasks in synthetic biology. </b> <i>Biotechnol. J. </i>6(7):784-95. </li><br>
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  • Team:Potsdam Bioware/Labjournal/May
    ...l 629 (digested) + 6 µl 6x loading dye solution, 30 µl 654 (digested) + 6 µl 6x loading dye<br> 0,5 g Agarose, 50 ml TAE (1%), 2 µl GelRed (3-6µl), at 100 Volt,running time: 30 minutes<br>
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  • Team:UANL Mty-Mexico/Project/Mechanism
    ...asic switch (figure 2)<a href="#References" class="references-link">[4][5][6]</a>, we decided to borrow the mechanism. The biphasic switch allows contro ...c gate called AND gate. That means it requires two inputs to be on (figure 6), red and green lights for this case.
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  • Team:HKUST-Hong Kong/asm.html
    background-image:url('https://static.igem.org/mediawiki/2011/6/63/Bottom.jpg'); background-attachment:fixed; border: none; margin: 0; padd <center><img src=https://static.igem.org/mediawiki/2011/6/68/Ust_Have_both.png width=770></center>
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  • Team:Bilkent UNAM Turkey/Protocols
    <br>CoCl2. 6 H2O 1.61 g 50 ml <br>6. To a tube containing 0.3g glass beads (sterilized by baking) add:
    14 KB (2,028 words) - 01:55, 22 September 2011
  • Team:USTC-China/Project/modeling
    <p>All codes are written in Python26(http://www.python.org/getit/releases/2.6/), you can download our codes here [[File:IGEM USTC 2011.zip]], you will ne ...;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;'''Fig. 6'''</p>
    21 KB (3,249 words) - 10:04, 28 October 2011
  • Team:DTU-Denmark-2/Project/background
    <font color=" #990000" face="arial" size="6"> ... their genome by recombination leading to a stable transfection of a gene (6). <br> </p<
    15 KB (2,211 words) - 08:40, 21 September 2011
  • Team:DTU-Denmark-2/results/background
    <font color=" #990000" face="arial" size="6"> ... their genome by recombination leading to a stable transfection of a gene (6). <br> </p<
    15 KB (2,208 words) - 14:44, 21 September 2011
  • Team:Amsterdam/Project/Modules
    ==6. Make and test a cold resistance backbone== Modules 4, 5 and 6 were not completed due lack of time and the logistic problems of the parts
    6 KB (880 words) - 20:39, 21 September 2011
  • Team:DTU-Denmark/Project improving araBAD
    ...ss="subscript">tet</span> promoter<span class="superscript">[[#References|[6]]]</span> or the propionate induced P<span class="subscript">prpB</span><sp ...oth the rationally designed promoters and SPL. '''Figure 5''' depicts that 6 of the mutated promoters are characterized by a higher activity than the or
    13 KB (2,036 words) - 03:54, 22 September 2011
  • Team:DTU-Denmark/Matlab
    event = gt(time,6), alpha_r, 0 event = gt(time,6), alpha_r, 0
    9 KB (1,357 words) - 04:08, 22 September 2011
  • Team:Osaka/week10
    ==October 6 (Thu)== ...week3|3]]|[[Team:Osaka/week4|4]]|[[Team:Osaka/week5|5]]|[[Team:Osaka/week6|6]]|[[Team:Osaka/week7|7]]|[[Team:Osaka/week8|8]]|[[Team:Osaka/week9|9]]|'''1
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  • Team:HKUST-Hong Kong/team.html
    background-image:url('https://static.igem.org/mediawiki/2011/6/63/Bottom.jpg'); background-attachment:fixed; border: none; margin: 0; padd <b>6. Jack</b><br />
    10 KB (1,556 words) - 14:34, 27 October 2011
  • Team:BYU Provo/Team OxyR/Week8
    ==June 6== Did another ligation identical to the one on June 6.
    840 B (117 words) - 23:18, 27 September 2011
  • Team:BYU Provo/Team Thermosensor/Week12
    ===7/6/11=== * T38-5 and 6: from colony 2 from plate ∆T30-2
    2 KB (403 words) - 08:32, 24 September 2011
  • Team:BYU Provo/Team Thermosensor/Week21
    ===September 6=== *Plates T61-2, T61-4, T61-6 (37C) sectors 7&8 blue while other sectors had hints of blue towards end of
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  • Team:USC/Notebook/Week8
    [[Team:USC/Notebook/Week6|Week 6]] [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
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  • Team:USC/Notebook/Week9
    [[Team:USC/Notebook/Week6|Week 6]] [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
    6 KB (839 words) - 03:57, 29 September 2011
  • Team:UT Dallas/protocols new
    ... type="disk">For long term storage, use 0.1M CaCl2 in 15% glycerol at step 6 and store cells at -800 degrees Celsius<p></p></li><p> ...0 µL Buffer N3 and mix immediately and thoroughly by inverting the tube 4-6 times</li><li type="disk">Centrifuge for 10 minutes at 13000 rpm (~17900g)
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  • Team:Peking R/Notebook/ZYY/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,437 words) - 17:24, 25 September 2011
  • Team:Peking R/Notebook/CSB/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,449 words) - 17:26, 25 September 2011
  • Team:Peking R/Notebook/LZ/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,438 words) - 17:20, 25 September 2011
  • Team:Peking R/Notebook/GY/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,442 words) - 17:30, 25 September 2011
  • Team:Peking R/Notebook/XQY/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,455 words) - 17:27, 25 September 2011
  • Team:Peking R/Notebook/LYP/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,432 words) - 17:21, 25 September 2011
  • Team:Peking R/Notebook/HSY/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,461 words) - 17:23, 25 September 2011
  • Team:Peking R/Notebook/YXW/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,460 words) - 17:14, 25 September 2011
  • Team:Peking R/Notebook/ZMQ/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,450 words) - 17:16, 25 September 2011
  • Team:Peking R/Notebook/MT/zmq
    <th width="125" rowspan="6" bgcolor="#DCF8A9" scope="row"><p><a href="https://2011.igem.org/Team:Pekin ..." bgcolor="#DCF8A9"><span class="sunday"><a href="#d706" class="clickteam">6</a></span></td>
    31 KB (4,438 words) - 17:18, 25 September 2011
  • Team:Calgary/Notebook/Calendar/LiveCell
    ...contain the invitrogen ladder, while 2, 16 and 20 contain an NEB ladder. 3-6 contain restriction digests. Nothing can be inferred from this gel.</p> <p><b>Figure 3.</b> Gel run using the PCR products. Lanes 2-6 are for the IPTG promoter followed by the firefly luciferase generator, whi
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  • Team:GeorgiaTech/Citations
    ...ic record of all prior encounters with foreign transgressors1, 2, 3, 4, 5, 6. CRISPRs are transcribed and the long primary transcript is processed into ...f the National Academy of Science of the United States</u> <b>108</b>(25): 6.<p>
    12 KB (1,753 words) - 03:56, 29 September 2011
  • Team:Calgary/Project/References
    ...for dunaliella salina by glass beads method. Molecular Biology Reports, 36(6), 1433-1439.</li> ...urkholderia thailandensis and burkholderia pseudomallei. Nature Protocols, 6(8), 1085-1104.
    9 KB (1,275 words) - 04:01, 29 October 2011
  • Team:Northwestern/Results/Summary
    ...S34+GFP, CP+RBS34+RhlR] was most suitable in autoinducer detection. Figure 6 shows our GR(S) promoter is sensitive to a range of autoinducer concentrati <caption align="bottom"></html>'''Figure 6: Dose response of the PAI-2 Biosensor System 2 (''GR(S)+RBS34+GFP, CP+RBS34
    25 KB (3,750 words) - 03:48, 29 October 2011
  • Team:USC/Notebook/Week14
    [[Team:USC/Notebook/Week6|Week 6]] [[File:week6.jpg|link=Team:USC/Notebook/Week6|Week 6]]
    10 KB (1,302 words) - 03:59, 29 September 2011
  • Team:Peking S/lab/notebook/lhc
    <font size=6> <font color="#FFFFFF">Hanchi Lin's Notebook</font></FONT> |style="text-align:center"| [[Team:Peking_S/lab/notebook/shd#7.3 - 7.11|6]]
    15 KB (2,050 words) - 00:17, 6 October 2011
  • Team:Peking S/lab/notebook/dln
    <font size=6> <font color="#FFFFFF">Linna Deng's Notebook</font></FONT> |style="text-align:center"| [[Team:Peking_S/lab/notebook/yrf#7.6|6]]
    12 KB (1,673 words) - 21:59, 5 October 2011
  • Team:Tokyo Tech/Projects/RPS-game/assay
    ...ttp://download.macromedia.com/pub/shockwave/cabs/flash/swflash.cab#version=6,0,0,0" width="965" height="150" id="Yourfilename" align=""> <li><a href="#6.">6. LsrR repression</a></li>
    43 KB (6,329 words) - 03:25, 29 October 2011
  • Team:NYMU-Taipei/immunological-solution
    ... cytokines, our team has performed ELISA to detect the concentration of IL-6 (Fig.1), cytokine secreted by microglia cells when the brain tissue is infe | Fig.1 Structure of IL-6<br>(From: http://www.pdb.org/pdb/explore/explore.do?structureId=1ALU)
    11 KB (1,726 words) - 23:22, 28 October 2011
  • Team:Peking S/project/blogic/extension
    <font color="#ffffff"> <font size=6>Boolean Logic</font></font> ... fitting result. The concentrations of arabinose are 1e-7, 3e-7, 1e-6, 3e-6, 1e-5, 3e-5, 1e-4, 3e-4, 1e-3, 3e-3 and 1e-2, respectively. Fluorescence v
    10 KB (1,532 words) - 01:50, 6 October 2011
  • Team:SJTU-BioX-Shanghai/Project/Subproject1/Design
    '''RFP-6AGG''': we have inserted 6 AGG codons after the start codon ATG in the RFP gene. ... of the number of rare codons inserted in the mRNA. We have inserted 2, 4, 6, 8 AGG codons respectively after the start codon in luciferase gene. T7 pr
    10 KB (1,485 words) - 05:33, 28 October 2011
  • Team:Peking S/project/wire/inverter
    <font color="#ffffff"> <font size=6>Chemical Wire Toolbox</font></font> ...nd its final concentration varied from 0 to 1mM/1μM. When OD600 reached 0.6-0.8, pellet bacterial cells by 4 min centrifugation at 4000 rpm, discard th
    13 KB (1,913 words) - 14:27, 22 November 2011
  • Team:USTC-China/Drylab/modeling
    <p>All codes are written in Python26(http://www.python.org/getit/releases/2.6/), you can download our codes here [[File:IGEM USTC 2011.zip]], you will ne ...;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;'''Fig. 6'''</p>
    21 KB (3,348 words) - 02:39, 29 October 2011
  • Team:VIT Vellore/Documentation
    ...plasmids have a high copy number, the growth time may be reduced to approx 6 h. • &nbsp;10mM Tris-HCl pH 7.6<br/>
    8 KB (1,307 words) - 22:11, 5 October 2011
  • Team:Hong Kong-CUHK/Project/light
    <div class="span-6 square"> ...de concentration right after light illumination to bacterial samples (Fig. 6, Fig. 7). After light illumination, the bacteria expressing <a href="http:/
    27 KB (4,251 words) - 18:23, 28 October 2011
  • Team:Fatih Turkey/sporicideprocedure
    background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat; <p>6. The bottles are allowed to cool comletely, caps are tightened, solutions a
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  • Team:Fatih Turkey/Notebook13
    background: url('https://static.igem.org/mediawiki/2011/6/69/Bar-title-sh-left.png') no-repeat; <li><a href="https://2011.igem.org/Team:Fatih_Turkey/Notebook6">6</a></li>
    18 KB (2,350 words) - 02:57, 29 October 2011
  • Team:Paris Bettencourt/Modeling/Assisted diffusion/From membrane tension to liquid flux
    * N<sub>1</sub> = 2*10<sup>6</sup>, N<sub>2</sub> = 1*10<sup>6</sup>, R<sub>1</sub> = R<sub>2</sub> = 0.5 µm; * N<sub>1</sub> = 1*10<sup>7</sup>, N<sub>2</sub> = 5*10<sup>6</sup>, R<sub>1</sub> = 2 µm, R<sub>2</sub> = 1 µm;
    7 KB (1,223 words) - 03:05, 29 October 2011
  • Team:CongoDRC-Bel Campus/Project
    ...e, 21 Member States (old and new foci) in Western (9 countries), Central (6 countries), Eastern (4 countries) and Southern Africa (2 countries) have r ...nal investigation has shown that there were some suspected cases of UB in 6 of 11 regions. Three(3) of these are considerated highest endemic: Bas con
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  • Workshops/Proposals
    <tr><td>US/Canada<td>$6,250<td>$4,000</tr> <tr><td>Europe<td>$6,250<td>$9,000^</tr>
    5 KB (819 words) - 02:16, 12 March 2011
  • Team:Lethbridge/Project
    ... hydrolyzes catechol into 2-HMS, which can enter the citric acid cycle<sup>6</sup>. In 2010, the Lethbridge iGEM team confirmed that xylE when introduce ...of the more toxic organic molecules found in the tailings ponds.<sup>3,4,5,6,10,24</sup>
    28 KB (4,177 words) - 16:21, 12 April 2012
  • Team:Greenfield IN-Rihm-HS/Notebook
    <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD> <TD vAlign=top align=left width="14%" ><font size="3" face="Verdana">6</font><br><br><br><br></TD>
    17 KB (2,661 words) - 02:32, 24 June 2011
  • Team:Grenoble/Notebook Rawhide
    <p>6 out of 8 sequence alignments failed.<br/> -> every 1st-step constructions that failed last time, so 6 constructions.<br/>
    15 KB (2,221 words) - 12:43, 8 August 2011
  • Team:Wageningen UR/Notebook/Proj1/July
    '''July 6''' ! no. 1 (6 hours)
    4 KB (535 words) - 17:59, 21 September 2011
  • Team:Alberta/Notebook
    ===May 6===
    3 KB (521 words) - 15:54, 13 May 2011
  • Team:Amsterdam/Notebook/Protocols/Purification
    ...dd 250 µl of the lysis solution and mix thoroughly by inverting the tub 4-6 times until the solution becomes viscous and slightly clear.</li> ... neutralization solution and mix immediately and thoroughly by inverting 4-6 times.</li>
    2 KB (324 words) - 19:50, 16 September 2011
  • Team:Yale/Notebook/Week2
    <li><a href="https://2011.igem.org/Team:Yale/Notebook/Week6">Week 6</a></li> ...added to a beaker. Volume was brought to 300mL. pH was brought to 6.7 with 6.25M KOH. 5.44g of MnCL2*4H2O (final concentration 55mM) was added, and the
    7 KB (1,260 words) - 18:21, 27 September 2011
  • Team:Michigan/Project
    ...e of Gli-1. CY3's excitation wavelength is 550 nm, and emission is 564 nm; 6-FAM excitation is 495 nm and emission is 520 nm. This method is a derivativ <img src="https://static.igem.org/mediawiki/2011/6/6e/FA_graph.jpg" width="100%" alt="300">
    8 KB (1,311 words) - 03:16, 28 October 2011
  • Team:SouthBend-Mishawaka-HS/Notebook
    ... ul of the diluted DNA in 50 ul of transformation solution (50mm CaCl2, pH 6.1 BIO-RAD Transformation Solution control # 31000008916 recieved 1-17-11 GT (6)Incubate on ice for 5 minutes<br>
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  • Team:Paris Liliane Bettencourt/Notebook/2011/07/01/
    </p><p style="display:block;"><font color="blue">Protocol</font> (6 wells) </p><p style="display:block;"><font color="blue">Protocol (attC gel)</font> (6 wells)
    7 KB (1,095 words) - 15:51, 27 August 2011
  • Team:EPF-Lausanne/Notebook/June2011
    == Monday, 6 June 2011 == ...L). The assembly was done with a sample of 5 microliters taken from the 11.6 microliter mix.
    19 KB (1,384 words) - 16:51, 9 July 2011
  • Team:Brown-Stanford/lightbox/js
    } else { // Explorer Mac...would also work in Explorer 6 Strict, Mozilla and Safari ...nt.documentElement && document.documentElement.clientHeight) { // Explorer 6 Strict Mode
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  • Team:Wageningen UR/Contact
    +31 6 3136 3576 +31 6 1065 6387
    751 B (91 words) - 22:24, 17 August 2011
  • Reporter Group Notebook
    [[Reporter: Week 4 June 6-11]] [[Reporter: Week 6 June 20-25]]
    3 KB (301 words) - 07:24, 28 September 2011
  • Team:EPF-Lausanne/Protocols/TetR
    ...orward: CTC GAG AAT TCG CCA CCA TGT CCA GAT TAG ATA AAA GTA AAG ; <b>Tm=62.6ºC</b> :<b>6.</b> 4°C "forever"
    3 KB (452 words) - 08:48, 15 July 2011
  • Team:Lyon-INSA-ENS/Realisation/Protocols/Isolation
    6 – 8 times. Do not vortex to avoid shearing of genomic tube 6 – 8 times. Do not vortex to avoid shearing of
    3 KB (481 words) - 10:20, 10 January 2012
  • Team:UNITS Trieste/Team
    radius: 6, <p>iGEM team <b>UNITS_trieste</b> is composed by 6 students 2 advisors and 4 instructors: a small team with a great idea!!<br/
    15 KB (2,135 words) - 16:50, 28 October 2011
  • Team:Caltech/References
    ...Anopheles dirus species B." Insect Biochemistry and Molecular Biology 28(5-6): 321-329. [http://www.sciencedirect.com/science/article/pii/S0965174898000 ...anding the synthetic biology toolbox for biomass conversion. Mol Syst Biol 6 (2010).
    5 KB (689 words) - 23:40, 28 September 2011
  • Team:BU Wellesley Software/Notebook/JanooNotebook/test
    <td>6</td> <td>6</td>
    3 KB (529 words) - 04:12, 27 June 2011
  • Team:Caltech/Week 1
    ==6/15==
    348 B (54 words) - 08:33, 20 June 2011
  • Team:Yale/Protein
    <center>Figure 2: Western Blot, probed with His-6 antibody; uninduced eGFP-TmAFP (left), induced eGFP-TmAFP (right)</center> ...ne 4: induced eGFP-RiAFP in Origami, Lane 5: uninduced RiAFP in BL21, Lane 6: induced RiAFP in BL21, Lane 7: uninduced RiAFP in Origami, Lane 8: induced
    8 KB (1,129 words) - 14:41, 28 October 2011
  • Team:Waterloo/Project
    ...luoresce, which is a consequence of some fusion protein techniques. Figure 6 shown below details the negative control design: [[File:Figure4.png|none|thumb|upright=3.1|alt=Negative|'''Figure 6''' is an illustration of the negative control containing the interrupted GF
    18 KB (2,786 words) - 17:06, 25 September 2011
  • Team:BU Wellesley Software/Notebook/ConsueloNotebook
    == Jul 6: Protocols DONE! ==
    9 KB (1,312 words) - 20:34, 28 September 2011
  • Team:UT Dallas/Protocols
    ...ype = "disk">For long term storage, use 0.1M CaCl2 in 15% glycerol at step 6 and store cells at -800 degrees Celsius</p></li><p> ...0 µL Buffer N3 and mix immediately and thoroughly by inverting the tube 4-6 times<li type = "disk">Centrifuge for 10 minutes at 13000 rpm (~17900g) in
    20 KB (2,977 words) - 02:11, 3 September 2011
  • Team:Fatih Turkey/anti-lps.html
    ...activation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974;6:422–425. [PMC free article] [PubMed]<br /> 6. Fletcher M A, Mckena T M, Quance J L, Wainwright N R, Williams T J. Lipop
    17 KB (2,204 words) - 21:16, 12 August 2011
  • Team:Caltech/Week 4
    ==July 6==
    4 KB (621 words) - 00:11, 12 July 2011
  • Team:Uppsala-Sweden/Project/Protocol
    * If pH is above 6.4, adjust pH DOWN to 6.4 with HCl 6 g NaCl
    12 KB (1,960 words) - 23:24, 21 September 2011
  • Team:Freiburg/Project/broken
    [http://partsregistry.org/Part:BBa_K098995 K098995]: 6 nt missing between RBS and the cI repressor possibly leading to a diminishe :::We are trying to insert the 6 nt and test the part again.
    2 KB (339 words) - 11:28, 18 July 2011
  • Sensor: Week 6 June 20-25
    ===Validate Assemblies from 6/22=== ===Sequence Assemblies from 6/22===
    3 KB (509 words) - 15:59, 3 July 2011
  • Si Chen's Lab Notebook
    [[6]] [[6]]
    4 KB (397 words) - 18:13, 2 July 2011
  • Nina Jiauye Zhu's Lab Notebook
    [[6]] [[6]]
    4 KB (397 words) - 20:16, 2 July 2011
  • Yuanwei Li's Lab Notebook
    [[6]] [[6]]
    5 KB (404 words) - 21:58, 2 July 2011
  • Team:British Columbia/Notebook/6 June 2011
    ==June 6 2011==
    563 B (84 words) - 03:59, 4 July 2011
  • Team:EPF-Lausanne/Protocols/Klenow dsDNA synthesis
    |20.6 µL * To set up the 384-well plate with 6 dilutions load 30uL 0.5% BSA dH2O into all columns except 1, 7, 13, 19.
    2 KB (295 words) - 17:05, 21 September 2011
  • Team:Brown-Stanford/Lab/Protocols/RegistryCompetency
    * adjust pH DOWN to 6.4 with 0.1N HCl if necessary ... buffer + 1 part 5x ligation adjustment buffer and checking pH to be 6.3 - 6.5
    7 KB (1,045 words) - 18:18, 28 September 2011
  • Team:Caltech/Week 7
    File:7-25pcrgel2.jpg|lane 1 NEB 2-log ladder, 2-4 pNT003 insert, 5 R0010, 6 K123003, 7 B0014, 8 blank, 9-11 GFP insert, 12 GFP constitutive promoter, 1 <td>23.6</td>
    9 KB (1,486 words) - 20:58, 18 August 2011
  • Team:DTU-Denmark/Design test
    ====== Heading level 6 ======
    3 KB (464 words) - 13:50, 5 September 2011
  • Team:Macquarie Australia/Project
    ...ower generation, responses to sunlight and position relative to shade [1,5,6,7,8,9]. Phytochrome proteins act as light sensors by absorbing red/far red ...rs for bacteria, mediating sensory responses to their light environment [4,6]. Bacteriophytochromes can also act as protein kinases triggered by light.
    11 KB (1,681 words) - 09:53, 5 October 2011
  • Team:Waterloo/Notebook
    '''Monday, June 6, 2011''' '''Wednesday July 6, 2011'''
    10 KB (1,522 words) - 01:01, 25 September 2011
  • Team:Harvard/Test Page
    facts[6]='"When split fluorescent protein meets Chuck Norris, there is no possibili ...ide product was absent. Furthermore, the yield of violacein production was 6-fold higher in the presence of DNA program coding for the linear order of e
    17 KB (2,307 words) - 20:14, 7 July 2011
  • Team:KAIST-Korea/Projects/report 1
    <img src="https://static.igem.org/mediawiki/2011/6/67/Ecasso-report1-eqn05.jpg" width="35%" alt="Equation 5" /><br> ...rg/mediawiki/2011/f/f1/Ecasso-report1-eqn06.jpg" width="30%" alt="Equation 6" />
    20 KB (3,060 words) - 19:53, 22 July 2011
  • Team:Nevada/Notebook/Temp2
    <font size=6>Temp Calender</font> =='''Week 6 - July 4th-10th'''==
    7 KB (1,152 words) - 18:53, 4 August 2011
  • Team:Cambridge/Protocols/DNA Gel Extraction
    ...QG and 1 volume of isopropanol to the reaction, mix, and proceed with step 6 of the protocol. Alternatively, use the MinElute Reaction Cleanup Kit. ... add 300 μl of Buffer QG to each 100 mg of gel. For >2% agarose gels, add 6 volumes of Buffer QG. The maximum amount of gel slice per QIAquick column i
    6 KB (968 words) - 15:17, 11 July 2011
  • Team:Peking R/Biosafety
    z-index:6; z-index:6;
    10 KB (1,430 words) - 13:21, 28 October 2011
  • Team:Bilkent UNAM Turkey/Notebook/Week7
    <!--[if IE 6]><style type="text/css">@import "/wiki/skins/igem/IE60Fixes.css?164";</styl ...</strong> Grow cells in SGII+NH4NO3 until they reach a density of 1-2 x 10^6/ml.
    14 KB (1,950 words) - 17:44, 9 July 2011
  • Team:USTC-China/Project
    ...sp;&nbsp;&nbsp;According to Figure 7 and comparing with the control(Figure 6), the engineered bacteria('''Application design''') actually can destroy th ...ntial logic circuit: a push-on push-off switch. Molecular Systems Biology, 6:350
    13 KB (1,964 words) - 23:49, 28 October 2011
  • Team:Tianjin/Project
    ... <a href="#t33"><img src="https://static.igem.org/mediawiki/2011/6/67/TJU-Project-Menu-3-3.png"></a> ... <a href="#t41"><img src="https://static.igem.org/mediawiki/2011/6/65/TJU-Project-Menu-4-1.png"></a>
    55 KB (8,077 words) - 01:02, 6 October 2011
  • Team:NTNU Trondheim/Journal/Calendar/August
    Så for å linke til 'Friday 24/6' må man linke til 'Friday_24.2F6' [[#Friday_24.2F6 | Friday 24/6]]
    1 KB (116 words) - 14:41, 18 August 2011
  • Team:HIT-Harbin/Project
    ...oduct is nutritionally rich in protein, calcium, riboflavin, vitamin B<sub>6</sub> and vitamin B<sub>12</sub><sup>[1]</sup>. In addition, consumers who ...occus lactis''. It has a long history of safe use in food production <sup>[6]</sup>. So we insert the ''nisI'' gene between XbaI and PstI. And then, we
    12 KB (1,816 words) - 15:29, 5 October 2011
  • Team:Cornell/Week 14
    ==Tuesday, September 6==
    1 KB (135 words) - 17:24, 18 September 2011
  • Team:Caltech/Week 5
    ... left to right: 1 ladder, 2 R0010, 3 K123000, 4 B0014 for pNT002, 5 R0040, 6 K123001, 7 B0014 for pNT003, 8 B0014 for pNT001]] <td>3.6</td>
    6 KB (1,056 words) - 18:25, 20 July 2011
  • Team:NCTU Formosa/humanpractice
    <img src="https://static.igem.org/mediawiki/2011/9/92/Human-6.JPG" width="300"></div></center> No [18] 6%<br>
    19 KB (2,664 words) - 13:43, 5 October 2011
  • Team:NTNU Trondheim/Journal/Calendar/July
    Så for å linke til 'Friday 24/6' må man linke til 'Friday_24.2F6' [[#Friday_24.2F6 | Friday 24/6]]
    1 KB (140 words) - 07:41, 1 August 2011
  • Team:KULeuven/Notebook
    <span class="notebook-weekno">Week 6</span><br> <span class="notebook-weekno">Week 6</span><br>
    30 KB (4,574 words) - 23:19, 28 October 2011
  • Team:KULeuven/Safety
    ...s adapted to live in the mammalian digestive system. <sup><a href="#1">[5, 6, 7]</a></sup> <br><br><h2>6. Conclusion</h2>
    26 KB (4,243 words) - 12:53, 27 October 2011
  • Team:NTNU Trondheim/Journal/Calendar/June
    Så for å linke til 'Friday 24/6' må man linke til 'Friday_24.2F6' [[#Friday_24.2F6 | Friday 24/6]]
    878 B (101 words) - 08:27, 29 July 2011
  • Team:KULeuven/SocialMedia&action=edit
    !colspan="6"| <html><font size="6">Americas</font></html> !colspan="6"| <html><font size="6">Asia</font></html>
    82 KB (9,599 words) - 14:01, 20 July 2011
  • Team:Freiburg/Notebook/14 July
    6. heat for 1-2 hours 37°C (6 hours if time) 6. Incubate&nbsp; 10-30 min at room temperature
    19 KB (2,138 words) - 00:55, 22 September 2011
  • Team:Alberta/Methodology/Protocols
    <li>Add approximately 6 x 10<sup>6</sup> cells to the each flask.</li> <li>Dissolve 16.6 g of MgCl<sub>2</sub>* 6 H<sub>2</sub>O and 10.0 g of CaCl<sub>2</sub> * 2 H<sub>2</sub>O in 100 mL
    18 KB (2,545 words) - 00:27, 29 September 2011
  • Team:Minnesota/Project
    ... ice crystals in supercooled water <sup>[[Team:Minnesota/Project#mararitis|6]]</sup>. INP has distinct domains: the N-terminal module is responsible for <p style="margin-right:20px; margin-left:20px;"><small>Figure 6. pUCBB-pTet-Cph8 was digested with XbaI and PstI and pUCBB-pBad-ho1-pcyA wa
    13 KB (1,991 words) - 03:43, 29 September 2011
  • Team:IvyTech-South Bend/Safety
    6. If there are spills or accidents report them immediately to the faculty ad 6 Contaminated samples (chemical, biological, glass, and radioactive wastes)
    7 KB (1,106 words) - 14:38, 15 July 2011
  • Team:Imperial College London/Notebook/week2
    ...s single stranded and mix and anneal) ~75-135 mins. Transform ~60 mins. ~6.5 hours! That is being optimistic! </p> ...is format. Can use the parts from miniprep a maximum of around 4 times. ~6 bp overhang. Low Tm (~10 degrees celsius). Ligase works best at 37 degree
    9 KB (1,411 words) - 16:54, 15 September 2011
  • Team:Cornell/Week 9
    :::32.6μL H2O ::::10.6μL AviTagged pZE12 vector backbone
    5 KB (726 words) - 17:23, 18 September 2011
  • Team:Washington/scripts/Lightbox
    } else { // Explorer Mac...would also work in Explorer 6 Strict, Mozilla and Safari ...nt.documentElement && document.documentElement.clientHeight) { // Explorer 6 Strict Mode
    19 KB (2,131 words) - 22:29, 20 July 2011
  • Team:Freiburg/Notebook/1 August
    # heat for 1-2 hours 37°C (6 hours if time) ...g-top:0in;padding-bottom:0in;padding-left:0.075in;padding-right:0.075in;"| 6
    22 KB (2,682 words) - 01:03, 22 September 2011
  • Team:Cornell/Results
    ::*Total Run Time - 6 hours ... VioA VioB VioE into prodeoxyviolacein. 1 ml samples were collected after 6 hours of continuous flow.
    16 KB (2,419 words) - 03:07, 29 October 2011
  • Anti-lps.html
    ...activation of endotoxin by polymyxin B. Antimicrob Agents Chemother. 1974;6:422–425. [PMC free article] [PubMed]<br /> 6. Fletcher M A, Mckena T M, Quance J L, Wainwright N R, Williams T J. Lipop
    17 KB (2,204 words) - 10:17, 7 August 2011
  • Team:Imperial College London/Brainstorming Bioremediation
    <p>[6] <a href="http://onlinelibrary.wiley.com/doi/10.1002/iroh.201111355/pdf" re ...degradation of bisphenol A leads to the production of corrosive compounds (6). It may therefore by preferable to use horseradish peroxidase, which appea
    14 KB (2,193 words) - 18:44, 21 September 2011
  • Team:NTNU Trondheim/Journal Modeling/Calendar/August
    Så for å linke til 'Friday 24/6' må man linke til 'Friday_24.2F6' [[#Friday_24.2F6 | Friday 24/6]]
    979 B (107 words) - 15:14, 14 September 2011
  • Team:Amsterdam/Notebook/Protocols/Miniprepping
    ...b> of the <b>lysis solution</b> and mix thoroughly by inverting the tube 4-6 times until the solution becomes viscous and slightly clear.</li> ...tralization solution</b> and mix immediately and thoroughly by inverting 4-6 times.</li>
    3 KB (467 words) - 23:59, 21 September 2011
  • Team:NTNU Trondheim/Journal Modeling/Calendar/July
    Så for å linke til 'Friday 24/6' må man linke til 'Friday_24.2F6' [[#Friday_24.2F6 | Friday 24/6]]
    1 KB (111 words) - 12:50, 31 July 2011
  • Team:NTNU Trondheim/Journal Modeling/Calendar/June
    Så for å linke til 'Friday 24/6' må man linke til 'Friday_24.2F6' [[#Friday_24.2F6 | Friday 24/6]]
    797 B (93 words) - 08:31, 29 July 2011
  • Team:Lyon-INSA-ENS/Realisation/Protocols/Miniprep
    <b>3.</b> Add 250µL buffer P2 and mix thoroughly by inverting the tybe 4-6 times until the solution becomes clear. Do not allow the lysis reaction to ...50µL buffer N3 and mix immediately and thoroughly by inverting the tube 4-6 times.
    3 KB (380 words) - 10:44, 10 January 2012
  • Team:UNIPV-Pavia/Calendar/July/settimana5
    <td class="row">6</td> ...png" class="image"><img alt="" src="https://static.igem.org/mediawiki/2011/6/66/UNIPV_25_07_2011_E24_E-P_E25_E-P_E26_E-P_E27-E-P.png" class="thumbimage"
    16 KB (2,562 words) - 14:47, 17 August 2011
  • Thursday, July 28
    LABLED 6 CENTERFUGE TUBES Removed 6 mutagenesis tubes from freezer labled as Mutagenesis P,R and T products gro
    718 B (110 words) - 00:06, 29 July 2011
  • Team:Paris Liliane Bettencourt/Notebook/2011/07/29/
    ... mL. Let them growth for 2-3h until they reach the OD600 between 0,3 and 0,6. 144,6 1.94
    2 KB (361 words) - 15:39, 1 September 2011
  • Team:Imperial College London/Brainstorming Others
    ...duction increased when all three enzymes were used on beechwood xylan. Lac-6 enhances glucose release from hemicellulose by cellulases? Microcrystalline <p>The idea of this project is to use bacteria to express Lac-6, β-glu and Cell-1 in order to produce monosaccharides from other sources o
    10 KB (1,756 words) - 18:54, 21 September 2011

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