Team:BYU Provo/Team Thermosensor/Week16

From 2011.igem.org

Team BYU Provo

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Week 16 (July 30 - Aug. 6)

6 August 2011

Turns out the thermosensor primers were bad bad bad. Dr. Grose performed a cloning using redesigned DeltaTS primers. I looked at the plate today, almost all colonies were big and blue! I set up the boiled template from 7 of the colonies to be able to do colony PCR and check for insertion. Tubes labeled boiled tamplate 47-1. plastic labeling on tubes. Pie slices on xgal plate which plate is in the 30oC incubator.