Team:BYU Provo/Team OxyR/Week5


Team BYU Provo

BYU Provo


May 16

Results of PCR amplification of OxyS, SoxS, KatG, and HemH promoters.

Amplified OxyS, SoxS, KatG, and HemH promoters and cleaned up the products with ethanol.

May 17

Restriction digested the products from the previous day.

May 18

Froze down bacterial strains: pGLOW, pPLAT, and pPLAT w/ GFP. Ligated above promoters into pPLAT w/ GFP and transformed them into DH5a E. Coli.

May 19

Prepared colony PCR of above transformed bacteria to ensure the ligation and transformation worked.