Team:BYU Provo/Team OxyR/Week13


Team BYU Provo

BYU Provo


18 July 2011

Matt and Rob came in and set up colony PCR to check the OxyR-Med ligations. Rob was the master of boiling/patching colonies.

19 July 2011

PCR results from July 18th:


20 July 2011

Purified plasmids from the most recent round of transformations (using the colonies that passed the colony PCR test).

21 July 2011

Performed another ligation trying the katG promoter again.

22 July 2011

Plates that Matt started (the ligation from July 21st) were only pinpricks in the morning. Rob allowed them to grow a bit longer.

23 July 2011

Mackay set up a colony PCR checking the KatG/OxyR-Medium construct again (the one that hadn't been working).