Team:BYU Provo/Team Thermosensor/Week23

From 2011.igem.org

Team BYU Provo

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- Team Thermosensor:
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Week 23 (Sep 18 - 23)

9/19/11

Restreaked all the colonies onto new ara/amp/Xgal plates to redo the experiment one last time. Placed in 30ºC incubator
Took colonies that looked promising and made overnights to do plasmid preps.

Examples of these plates that were double checked.

IGM45, our wild-type thermosensor is in upper left quadrant (9 o'clock-10 o'clock space).

IGM45 vs. Hour 1 thermosensors, after experimentation

IGM45 vs. Hour 5 thermosensors, after experimentation

IGM45 vs. Hour 8 thermosensors, after experimentation

9/20/11

Plasmid prep on 14 of the colonies to sequence our thermosensor

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