Team:BYU Provo/Team OxyR/Week10

From 2011.igem.org

Team BYU Provo

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June 20

Results from plasmid purification.

Purified plasmid from overnights containing SoxS and HemH with the weak, medium, and strong promoters for SoxR and OxyR, respectively.

June 22

Did colony PCR to find OxyR medium and SoxS strong constructs.

June 23

Electroporation: plasmids with GFP only and GFP with the SoxS promoter into SoxR- strains; plasmids with GFP only and GFP with either the HemH and KatG promoters into OxyR- minus strains; plasmids with GFP only and GFP with one of the SoxS, HemH, and KatG promoters into wild-type E. Coli strains.