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From 2011.igem.org

Kit components Product Ethidium Bromide Solution, 10ml Product code H5041 Substance number Description Amount Symbols H504 Ethidium Bromide Solution 1 T

Page 1/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 DR

• 1 Identification of the substance/mixture and of the company/undertaking

Product identifier Trade name: Ethidium Bromide Solution Article number: H504 Application of the substance / the preparation Laboratory chemicals Details of the supplier of the safety data sheet Manufacturer/Supplier: Promega Corporation 2800 Woods Hollow Road Madison, WI 53711 U.S.A. 1-800-356-9526 or (608)-274-4330 Information department: MSDS author: Regulatory.Affairs@promega.com Emergency telephone number: For Chemical Emergency ONLY (spill, leak, fire, exposure or accident), call CHEMTREC at 1-800-424-9300 For call originating outside the United States dial 001-703-527-3887 2 Composition/information on ingredients Chemical characterization: Mixtures Description: Mixture of the substances listed below with nonhazardous additions. Dangerous components: Ethidium bromide <2.00% Additional information: For the wording of the listed risk phrases refer to section 15.

• 3 Hazards identification

Classification of the substance or mixture Classification according to Directive 67/548/EEC or Directive 1999/45/EC Toxic Toxic by inhalation. Harmful Possible risk of irreversible effects. Classification system: The classification was made according to the latest editions of international substances lists, and is expanded upon by company and technical literature data. Label elements Labelling according to EU guidelines: The product has been classified and marked in accordance with directives on hazardous materials. (Contd. on page 2) USA Page 2/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 Trade name: Ethidium Bromide Solution (Contd. of page 1) DR Code letter and hazard designation of product: T Toxic Hazard-determining components of labelling: Ethidium bromide Risk phrases: Toxic by inhalation. Possible risk of irreversible effects. Safety phrases: Keep away from living quarters. Keep container in a well-ventilated place. When using do not eat or drink. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). This material and its container must be disposed of as hazardous waste. Classification system: NFPA ratings (scale 0 - 4) Health = 3 Fire = 0 Reactivity = 0 HMIS-ratings (scale 0 - 4) Health = 3 Fire = 0 Reactivity = 0 OSHA Hazard Overview (Criteria according to 29CFR1910.1200): Highly Toxic Primary route(s) of entry: Inhalation Oral Target Organ(s): Not applicable or unknown

• 4 First aid measures

General information: Immediately remove any clothing soiled by the product. Remove breathing apparatus only after contaminated clothing have been completely removed. In case of irregular breathing or respiratory arrest provide artificial respiration. Take affected persons out into the fresh air. Do not leave affected persons unattended. Seek medical treatment. Provide oxygen treatment if affected person has difficulty breathing. Medical supervision for at least 48 hours. After inhalation: Supply fresh air or oxygen; call for doctor. In case of unconsciousness place patient stably in side position for transportation. Call a doctor immediately. After skin contact: Immediately wash with water and soap and rinse thoroughly. After eye contact: Rinse opened eye for several minutes under running water. After swallowing: If symptoms persist consult doctor. USA (Contd. on page 3) Page 3/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 Trade name: Ethidium Bromide Solution (Contd. of page 2) DR

• 5 Firefighting measures

Suitable extinguishing agents: CO2, extinguishing powder or water spray. Fight larger fires with water spray or alcohol resistant foam. Special hazards arising from the substance or mixture None known Protective equipment: Mouth respiratory protective device.

• 6 Accidental release measures

Personal precautions, protective equipment and emergency procedures Keep people at a distance and stay upwind. Wear protective clothing. Environmental precautions: Dilute with plenty of water. Do not allow to enter sewers/ surface or ground water. Methods and material for containment and cleaning up: Absorb with liquid-binding material (sand, diatomite, acid binders, universal binders, sawdust). Dispose contaminated material as waste according to item 13. Ensure adequate ventilation. Reference to other sections See Section 7 for information on safe handling. See Section 13 for disposal information.

• 7 Handling and storage

Handling: Precautions for safe handling Keep receptacles tightly sealed. Ensure good ventilation/exhaustion at the workplace. Open and handle receptacle with care. Prevent formation of aerosols. Work only in fume cabinet. Information about protection against explosions and fires: Keep respiratory protective device available. The product is not flammable. Storage: Requirements to be met by storerooms and receptacles: No special requirements. Information about storage in one common storage facility: Not required. Further information about storage conditions: Keep receptacle tightly sealed. Specific end use(s) No further relevant information available. 8 Exposure controls/personal protection Components with limit values that require monitoring at the workplace: The product does not contain any relevant quantities of materials with critical values that have to be monitored at the workplace. Additional information: The lists that were valid during the creation were used as basis. Personal protective equipment: General protective and hygienic measures: Keep away from foodstuffs, beverages and feed. Immediately remove all soiled and contaminated clothing. (Contd. on page 4) USA Page 4/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 Trade name: Ethidium Bromide Solution (Contd. of page 3) DR Wash hands before breaks and at the end of work. Store protective clothing separately. Do not eat or drink while working. Ensure that washing facilities are available at the work place. Breathing equipment: In case of brief exposure or low pollution use respiratory filter device. In case of intensive or longer exposure use respiratory protective device that is independent of circulating air. Protection of hands: Protective gloves Selection of the glove material on consideration of the penetration times, rates of diffusion and the degradation Material of gloves The selection of the suitable gloves does not only depend on the material, but also on further marks of quality and varies from manufacturer to manufacturer. As the product is a preparation of several substances, the resistance of the glove material can not be calculated in advance and has therefore to be checked prior to the application. Eye protection: Goggles recommended during refilling.

• 9 Physical and chemical properties

General Information Appearance: Form: Fluid Color: Colorless Odor: Characteristic Odour threshold: Not determined. pH-value: Not determined. Change in condition Melting point/Melting range: 0°C (32 °F) Boiling point/Boiling range: 100°C (212 °F) Flash point: Not applicable. Flammability (solid, gaseous): Not applicable. Ignition temperature: Decomposition temperature: Not determined. Auto igniting: Product is not selfigniting. Danger of explosion: Product does not present an explosion hazard. Explosion limits: Lower: Not determined. Upper: Not determined. Vapor pressure: Not determined. Density at 20°C (68 °F): 1 g/cm³ (8.345 lbs/gal) Relative density Not determined. Vapour density Not determined. Evaporation rate Not determined. Solubility in / Miscibility with Water: Fully miscible. Segregation coefficient (n-octonol/water): Not determined. (Contd. on page 5) USA Page 5/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 Trade name: Ethidium Bromide Solution (Contd. of page 4) DR Viscosity: Dynamic: Not determined. Kinematic: Not determined. Solvent content: Organic solvents: 0.0 % Water: 99.0 % Solids content: 1.0 % Other information No further relevant information available.

• 10 Stability and reactivity

Thermal decomposition / conditions to be avoided: No decomposition if used according to specifications. Incompatible materials: No further relevant information available. Hazardous decomposition products: No dangerous decomposition products known. 11 Toxicological information Acute toxicity: LD/LC50 values that are relevant for classification: No data available Primary irritant effect: on the skin: No irritant effect. on the eye: Irritating effect. Sensitization: No sensitizing effects known. Additional toxicological information: The product shows the following dangers according to internally approved calculation methods for preparations: Toxic

• 12 Ecological information

Aquatic toxicity: Not harmful to the aquatic environment Persistence and degradability Not available Behavior in environmental systems: Bioaccumulative potential Not known Ecotoxical effects: Remark: Not available Additional ecological information: General notes: Water hazard class 1 (Self-assessment): slightly hazardous for water Do not allow undiluted product or large quantities of it to reach ground water, water course or sewage system. 13 Disposal considerations Waste treatment methods Recommendation: Disposal should be in accordance with applicable regional, national and local laws and regulations. Refer to Section 7: Handling and Storage and Section 8: Exposure Control/Personal Protection for additional handling information and protection of employees. (Contd. on page 6) USA Page 6/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 Trade name: Ethidium Bromide Solution (Contd. of page 5) DR Uncleaned packagings: Recommendation: Disposal must be made according to official regulations. Recommended cleansing agent: Water, if necessary with cleansing agents.

• 14 Transport information

Contact Promega Safety Department for additional transportation information DOT regulations: Not regulated Hazard class: - Land transport ADR/RID (cross-border): Not regulated ADR/RID class: - UN-Number: Not regulated Maritime transport IMDG: IMDG Class: - Marine pollutant: No Air transport ICAO-TI and IATA-DGR: Not regulated ICAO/IATA Class: -

• 15 Regulatory information

Sara Section 355 (extremely hazardous substances): None of the ingredients are listed. Section 313 (Specific toxic chemical listings): None of the ingredients are listed. TSCA (Toxic Substances Control Act): 7732-18-5 water, pure Proposition 65 Chemicals known to cause cancer: None of the ingredients are listed. Chemicals known to cause reproductive toxicity for females: None of the ingredients are listed. Chemicals known to cause reproductive toxicity for males: None of the ingredients are listed. Chemicals known to cause developmental toxicity: None of the ingredients are listed. Cancerogenity categories EPA (Environmental Protection Agency) None of the ingredients are listed. IARC (International Agency for Research on Cancer) None of the ingredients are listed. (Contd. on page 7) USA Page 7/7 Material Safety Data Sheet acc. to ISO/DIS 11014 Printing date 03/08/2011 Reviewed on 03/08/2011 Trade name: Ethidium Bromide Solution (Contd. of page 6) DR NTP (National Toxicology Program) None of the ingredients are listed. TLV (Threshold Limit Value established by ACGIH) None of the ingredients are listed. MAK (German Maximum Workplace Concentration) Ethidium bromide 3B NIOSH-Ca (National Institute for Occupational Safety and Health) None of the ingredients are listed. OSHA-Ca (Occupational Safety & Health Administration) None of the ingredients are listed. Product related hazard informations: The product has been classified and marked in accordance with directives on hazardous materials. Hazard symbols: T Toxic Hazard-determining components of labelling: Ethidium bromide Risk phrases: Toxic by inhalation. Possible risk of irreversible effects. Safety phrases: Keep away from living quarters. Keep container in a well-ventilated place. When using do not eat or drink. Wear suitable protective clothing, gloves and eye/face protection. In case of accident or if you feel unwell, seek medical advice immediately (show the label where possible). This material and its container must be disposed of as hazardous waste. National regulations: Class Share in % Wasser 99.0 I 1.0 Water hazard class: Water hazard class 1 (Self-assessment): slightly hazardous for water. 16 Other information This information is based on our present knowledge. However, this shall not constitute a guarantee for any specific product features and shall not establish a legally valid contractual relationship. Department issuing MSDS: Promega Corporation Environmental Health and Safety Department 2800 Woods Hollow Road Madison, WI Ph:(608)274-4330

• Data compared to the previous version altered.

USA