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From 2011.igem.org

pET15b OmpA-GFP construct

 Digest GFP vectors with NcoI (NEB) + CIP (NEB) 37 centigrade, 3hrs.
 Recycle the vector via isopropanol precipitation.
 Ligation
 5ul vector + 2ul OmpA inserts + Takara solution I mix. RT 1h
 Transform DH5a with all the reaction mix, spread on LB plates + Ampicillin.
 YX