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Team:Freiburg/Notebook/18 July
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This is the wiki page of the Freiburger student team competing for iGEM 2011. Thank you for your interest!
Precipitator
PCR
| Name:
Ruediger | Date:
18.07.2011 |
| Continue from Experiment (Date)PCR 1507
(Name) Ruediger | |
| Project Name:
GFP Pbd | |
PCR-Mixture for one Reaction:
For a 50 µl reaction use
| 32,5µl | H20 | |
| 10µl | 5x Phusion Buffer | |
| 2.5µl | Primer fw | |
| 2.5µl | Primer dw | |
| 1µl | dNTPs | |
| 1µl | DNA-Template | |
| 0.5 µl | Phusion (add in the end) |
What program do you use?
10x (95C-41C/47C/52C-72C) + 25x ((95C-60C-72C)
How did you label the PCR-Product, where is it stored and what do you do next?
Reactions:
P28+P18+M14.1
P28+P19+M14.1
P28+P20+M14.1
P28+P2+M14.1
P28+P3+M14.1
P1+P18+M14.1
P1+P19+M14.1
P1+P20+M14.1
P1+P2+M14.1
P1+P3+M14.1
P1+P3+M14.2
Upper panel:All lanes at 41C
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
| Quick Load Marker | P28+P18+M14.1 | P28+P19+M14.1 | P28+P20+M14.1 | P28+P2+M14.1 | P1+P19+M14.1 | P1+P20+M14.1 | P1+P2+M14.1
|
Lower Panel
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
| Quick Load Marker | P1+P3+M14.1
41C | P1+P3+M14.1
47C | P1+P3+M14.2
52C | P1+P3+M14.1
52C | P28+P3+M14.1
41C | P1+P18+M14.1
41C | P28+P3+M14.1
47C | P28+P3+M14.1
52C |
Upper Panel: all at 47C
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
| Quick Load Marker | P28+P18+M14.1 | P28+P19+M14.1 | P28+P20+M14.1 | P28+P2+M14.1 | P1+P18+M14.1 | P1+P19+M14.1 | P1+P20+M14.1 | P1+P2+M14.1 |
Lower Panel: all at 52C
| Lane 1 | Lane 2 | Lane 3 | Lane 4 | Lane 5 | Lane 6 | Lane 7 | Lane 8 | Lane 9 | Lane 10 |
| Quick Load Marker | P28+P18+M14.1 | P28+P19+M14.1 | P28+P20+M14.1 | P28+P2+M14.1 | P1+P18+M14.1 | P1+P19+M14.1 | P1+P20+M14.1 | P1+P2+M14.1 |
