Team:BYU Provo/Team Thermosensor/Week22

From 2011.igem.org

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    ===9/12/11===
 +
        *Plates moved
 +
            - Streak plates moved from 30º to 37º @ 7:15 AM
 +
                Blue colonies marked w/ a “B”
 +
            - 63 2-11 Moved to 37º @ 7:25
 +
            - 63-12, 13 and 19 are contaminated
 +
            - 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45
 +
            - “Hot flash” plates not moved
 +
        *Use red to mark the hours
 +
        *Plates checked at 10:00 AM (Hour 2)
 +
        *Plates checked at 12:00 (Hour 4) - Devin
 +
        *Plates checked at 1:00 Hour 5 -Devin
 +
        *Plates checked at 2:00 Hour 6 - Mark
 +
            - Look at 5-1 at 3:00
 +
        *Plates checked at 3:00 - Mark
 +
            - 5-1 (in corner on racks) is starting to look blue
 +
        *Checked at 4 PM - Chet hour 8
 +
        *Checked at 5 PM - Mark hour 9
 +
        *Checked at 6 PM - Chet hour 10
 +
        *At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
 +
        *Marked with an “M” the colonies that are now blue in the 42º
 +
        *Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue
 +
    ===9/14/11===
 +
        *Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
 +
        *Removed 37º-->42º plates
 +
        *Plate 66-1 (hour 8 colonies)
 +
            - Repeat check
 +
            - 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º
 +
        *66-2
 +
            - Same as above but for 30º-->42º hours 4 and 5
 +
        *66-3
 +
            - Same as above but for hours 8-10
 +
        *All left at room temp overnight
 +
        *Plated all the old and new blue colonies from the plates that we watched change hour by hour
 +
        *The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A
 +
            - “A” means they were from the new batch
 +
        *Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment
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Revision as of 20:53, 28 September 2011

Team BYU Provo

BYU Provo
 

   ===9/12/11===
       *Plates moved
           - Streak plates moved from 30º to 37º @ 7:15 AM
               Blue colonies marked w/ a “B”
           - 63 2-11 Moved to 37º @ 7:25
           - 63-12, 13 and 19 are contaminated
           - 63 12-20 placed in 45º (42º?) in Grose’s lab @ 7:45
           - “Hot flash” plates not moved
       *Use red to mark the hours
       *Plates checked at 10:00 AM (Hour 2)
       *Plates checked at 12:00 (Hour 4) - Devin
       *Plates checked at 1:00 Hour 5 -Devin
       *Plates checked at 2:00 Hour 6 - Mark
           - Look at 5-1 at 3:00
       *Plates checked at 3:00 - Mark
           - 5-1 (in corner on racks) is starting to look blue
       *Checked at 4 PM - Chet hour 8
       *Checked at 5 PM - Mark hour 9
       *Checked at 6 PM - Chet hour 10
       *At 6 PM, plates 2-1 were placed in 42º incubator in Dr. Grose’s lab after being marked.
       *Marked with an “M” the colonies that are now blue in the 42º
       *Colonies 5-5-10-10 may have been on plates without enough x-gal in them or something, because not even the controls turned blue
   ===9/14/11===
       *Moved room temp plates to 42º 8:30 AM, leave in til 10 AM
       *Removed 37º-->42º plates
       *Plate 66-1 (hour 8 colonies)
           - Repeat check
           - 37º-->42º Start plates incubated at 37º then moved to 42º. Selecting colonies from original plates that were not blue at 37º but turned blue at 42º
       *66-2
           - Same as above but for 30º-->42º hours 4 and 5
       *66-3
           - Same as above but for hours 8-10
       *All left at room temp overnight
       *Plated all the old and new blue colonies from the plates that we watched change hour by hour
       *The colonies that changed from 37º-->42º are labeled 42-1A-->42-10A
           - “A” means they were from the new batch
       *Put in 30º incubator. Dr. Grose would like to see them before we start the next phase of the experiment