Team:BYU Provo/Team OxyR/Week10

From 2011.igem.org

Revision as of 23:42, 27 September 2011 by Mackayam (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Team BYU Provo

Home
Team
Project
Results
Protocols
Parts Submitted
Modeling
Safety
Attributions
Notebooks:
- Team OxyR:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
  - Week 25
  - Week 26
  - Week 27
  - Week 28
- Team Thermosensor:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
Indiana Trip
Outreach

June 20

Results from plasmid purification.

Purified plasmid from overnights containing SoxS and HemH with the weak, medium, and strong promoters for SoxR and OxyR, respectively.

June 22

Did colony PCR to find OxyR medium and SoxS strong constructs.

June 23

Electroporation: plasmids with GFP only and GFP with the SoxS promoter into SoxR- strains; plasmids with GFP only and GFP with either the HemH and KatG promoters into OxyR- minus strains; plasmids with GFP only and GFP with one of the SoxS, HemH, and KatG promoters into wild-type E. Coli strains.