Team:BYU Provo/Team Thermosensor/Week3

From 2011.igem.org

Revision as of 02:23, 24 September 2011 by Cheddar3210 (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)

Team BYU Provo

Home
Team
Project
Results
Protocols
Parts Submitted
Modeling
Safety
Attributions
Notebooks:
- Team OxyR:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
  - Week 25
  - Week 26
  - Week 27
  - Week 28
- Team Thermosensor:
  - The Beginning
  - Week 1
  - Week 2
  - Week 3
  - Week 4
  - Week 5
  - Week 6
  - Week 7
  - Week 8
  - Week 9
  - Week 10
  - Week 11
  - Week 12
  - Week 13
  - Week 14
  - Week 15
  - Week 16
  - Week 17
  - Week 18
  - Week 19
  - Week 20
  - Week 21
  - Week 22
  - Week 23
  - Week 24
Indiana Trip
Outreach

Week 3 Thermosensor

5/2/11

Ran gel on colony PCR products. Faint bands that are a bit above 1500bp are visible. Much bolder bands visible further down the gel. Not sure if pBAD inserted or not. Set up overnights using colonies 4 and 5 (the faint bands were darkest here). We plan to have these colonies sequenced to find out for sure if pBAD did insert.

5/4/11

Left overnights in for two nights! Going to proceed with plasmid prep—it’s up to QIAgen now. Set up PCR to amplify LacZ, used phusion polymerase. Lengthened extension time to six minutes on PCR machine. Used plasmid JHG526 as our template DNA.